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Metabolic profiling of human blood

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Abstract

Metabolomics, the youngest branch of “omics” sciences, intended for studying a comprehensive set of low molecular weight substances (metabolites) of various biological objects. Metabolite profiles represent a molecular phenotype of biological systems and reflect information encoded at the genome level and realized at the transcriptome and proteome levels. Analysis of the human blood metabolic profile is a universal and promising tool for clinical applications because it is a sensitive measure of both endogenous and exogenous (environmental) factors influencing the human body. In this review we discuss technical implementation of blood metabolic profiling methods and statistic analysis of metabolite profiles for effective diagnostics and risk assessments of diseases.

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References

  1. Nicholson, J.K. and Lindon, J.C., Nature, 2008, vol. 455, pp. 1054–1056.

    Article  CAS  Google Scholar 

  2. Atherton, H.J., Gulston, M.K., Bailey, N.J., Cheng, K.K., Zhang, W., Clarke, K., and Griffin, J.L., Mol. Syst. Biol., 2009, vol. 5, p. 259.

    Article  Google Scholar 

  3. Schnackenberg, L.K., Expert Rev. Mol. Diagn., 2007, vol. 7, pp. 247–259.

    Article  CAS  Google Scholar 

  4. Richards, S.E., Dumas, M.E., Fonville, J.M., Ebbels, T.M.D., Holmes, E., and Nicholson, J.K., Chemometrics and Intelligent Laboratory Systems, 2010, vol. 104, pp. 121–131.

    Article  CAS  Google Scholar 

  5. Dunn, W.B., Broadhurst, D.I., Atherton, H.J., Goodacre, R., and Griffin, J.L., Chem. Soc. Rev., 2011, vol. 40, pp. 387–426.

    Article  CAS  Google Scholar 

  6. Barrett, D., Bioanalysis, 2012, vol. 4, pp. 643–644.

    Article  CAS  Google Scholar 

  7. Schnackenberg, L.K. and Beger, R.D., Pharmacogenomics, 2006, vol. 7, pp. 1077–1086.

    Article  CAS  Google Scholar 

  8. Kell, D.B. and Oliver, S.G., Bioessays, 2004, vol. 26, pp. 99–105.

    Article  Google Scholar 

  9. Psychogios, N., Hau, D.D., Peng, J., Guo, A.C., Mandal, R., Bouatra, S., Sinelnikov, I., Krishnamurthy, R., Eisner, R., Gautam, B., Young, N., Xia, J., Knox, C., Dong, E., Huang, P., Hollander, Z., Pedersen, T.L., Smith, S.R., Bamforth, F., Greiner, R., McManus, B., Newman, J.W., Goodfriend, T., and Wishart, D.S., PLoS One, 2011, vol. 6, p. e16957.

    Article  CAS  Google Scholar 

  10. Brindle, J.T., Antti, H., Holmes, E., Tranter, G., Nicholson, J.K., Bethell, H.W., Clarke, S., Schofield, P.M., McKilligin, E., Mosedale, D.E., and Grainger, D.J., Nat. Med., 2002, vol. 8, pp. 1439–1444.

    Article  CAS  Google Scholar 

  11. Kirschenlohr, H.L., Griffin, J.L., Clarke, S.C., Rhydwen, R., Grace, A.A., Schofield, P.M., Brindle, K.M., and Metcalfe, J.C., Nat. Med., 2006, vol. 12, pp. 705–710.

    Article  CAS  Google Scholar 

  12. Vaidyanathan, S., Kell, D.B., and Goodacre, R., J. Am. Soc. Mass. Spectrom., 2002, vol. 13, pp. 118–128.

    Article  CAS  Google Scholar 

  13. Ellis, D.I., Dunn, W.B., Griffin, J.L., Allwood, J.W., and Goodacre, R., Pharmacogenomics, 2007, vol. 8, pp. 1243–1266.

    Article  CAS  Google Scholar 

  14. Schauer, N., Steinhauser, D., Strelkov, S., Schomburg, D., Allison, G., Moritz, T., Lundgren, K., Roessner-Tunali, U., Forbes, M.G., Willmitzer, L., Fernie, A.R., and Kopka, J., FEBS Lett., 2005, vol. 579, pp. 1332–1337.

    Article  CAS  Google Scholar 

  15. Begley, P., Francis-McIntyre, S., Dunn, W.B., Broadhurst, D.I., Halsall, A., Tseng, A., Knowles, J., Goodacre, R., and Kell, D.B., Anal. Chem., 2009, vol. 81, pp. 7038–7046.

    Article  CAS  Google Scholar 

  16. Wang, X., Sun, H., Zhang, A., Wang, P., and Han, Y., J. Sep. Sci., 2001, vol. 34, pp. 3451–3459.

    Article  Google Scholar 

  17. Wilson, I.D., Nicholson, J.K., Castro-Perez, J., Granger, J.H., Johnson, K.A., Smith, B.W., Plumb, R.S., J. Proteome Res., 2005, vol. 4, pp. 591–598.

    Article  CAS  Google Scholar 

  18. Wang, Y., Lehmann, R., Lu, X., Zhao, X., and Xu, G., J. Chromatogr. A, 2008, vol. 1204, pp. 28–34.

    Article  CAS  Google Scholar 

  19. Monton, M.R. and Soga, T., J. Chromatogr. A, 2007, vol. 1168, pp. 237–246; discussion 236.

    Article  CAS  Google Scholar 

  20. Baidoo, E.E., Benke, P.I., Neususs, C., Pelzing, M., Kruppa, G., Leary, J.A., and Keasling, J.D., Anal. Chem., 2008, vol. 80, pp. 3112–3122.

    Article  CAS  Google Scholar 

  21. Beckwith-Hall, B.M., Nicholson, J.K., Nicholls, A.W., Foxall, P.J., Lindon, J.C., Connor, S.C., Abdi, M., Connelly, J., and Holmes, E., Chem. Res. Toxicol., 1998, vol. 11, pp. 260–272.

    Article  CAS  Google Scholar 

  22. Goodacre, R., Vaidyanathan, S., Dunn, W.B., Harrigan, G.G., and Kell, D.B., Trends Biotechnol., 2004, vol. 22, pp. 245–252.

    Article  CAS  Google Scholar 

  23. Scholz, M., Gatzek, S., Sterling, A., Fiehn, O., and Selbig, J., Bioinformatics, 2004, vol. 20, pp. 2447–2454.

    Article  CAS  Google Scholar 

  24. Smilde, A.K., Jansen, J.J., Hoefsloot, H.C., Lamers, R.J., van der Greef, J., and Timmerman, M.E., Bioinformatics, 2005, vol. 21, pp. 3043–3048.

    Article  CAS  Google Scholar 

  25. Vis, D.J., Westerhuis, J.A., Smilde, A.K., and van der Greef, J., BMC Bioinformatics, 2007, vol. 8, p. 322.

    Article  Google Scholar 

  26. Jonsson, P., Bruce, S.J., Moritz, T., Trygg, J., Sjostrom, M., Plumb, R., Granger, J., Maibaum, E., Nicholson, J.K., Holmes, E., and Antti, H., Analyst, 2005, vol. 130, pp. 701–707.

    Article  CAS  Google Scholar 

  27. Linden, A., J. Eval. Clin. Pract., 2006, vol. 12, pp. 132–139.

    Article  Google Scholar 

  28. Westergren, A., Karlsson, S., Andersson, P., Ohlsson, O., and Hallberg, I.R., J. Clin. Nurs., 2001, vol. 10, pp. 257–269.

    Article  CAS  Google Scholar 

  29. Bland, J.M. and Altman, D.G., BMJ, 2000, vol. 320, p. 1468.

    Article  CAS  Google Scholar 

  30. Broeckling, C.D., Reddy, I.R., Duran, A.L., Zhao, X., and Sumner, L.W., Anal. Chem., 2006, vol. 78, pp. 4334–4341.

    Article  CAS  Google Scholar 

  31. Baran, R., Kochi, H., Saito, N., Suematsu, M., Soga, T., Nishioka, T., Robert, M., and Tomita, M., BMC Bioinformatics, 2006, vol. 7, p. 530.

    Article  Google Scholar 

  32. Luedemann, A., Strassburg, K., Erban, A., and Kopka, J., Bioinformatics, 2008, vol. 24, pp. 732–737.

    Article  CAS  Google Scholar 

  33. Denkert, C., Budczies, J., Weichert, W., Wohlgemuth, G., Scholz, M., Kind, T., Niesporek, S., Noske, A., Buckendahl, A., Dietel, M., and Fiehn, O., Mol. Cancer., 2008, vol. 7, p. 72.

    Article  Google Scholar 

  34. Burtis, C.A., Ashwood, E.R., Bruns, D.E., and Tietz, N.W., Tietz fundamentals of clinical chemistry, St. Louis, Mo.: Saunders Elsevier, 2008.

    Google Scholar 

  35. Wishart, D.S., Tzur, D., Knox, C., Eisner, R., Guo, A.C., Young, N., Cheng, D., Jewell, K., Arndt, D., Sawhney, S., Fung, C., Nikolai, L., Lewis, M., Coutouly, M.A., Forsythe, I., Tang, P., Shrivastava, S., Jeroncic, K., Stothard, P., Amegbey, G., Block, D., Hau, D.D., Wagner, J., Miniaci, J., Clements, M., Gebremedhin, M., Guo, N., Zhang, Y., Duggan, G.E., Macinnis, G.D., Weljie, A.M., Dowlatabadi, R., Bamforth, F., Clive, D., Greiner, R., Li, L., Marrie, T., Sykes, B.D., Vogel, H.J., and Querengesser, L., Nucleic Acids Res., 2007, vol. 35 (Database issue), pp. D521–526.

    Article  CAS  Google Scholar 

  36. Wishart, D.S., Knox, C., Guo, A.C., Eisner, R., Young, N., Gautam, B., Hau, D.D., Psychogios, N., Dong, E., Bouatra, S., Mandal, R., Sinelnikov, I., Xia, J., Jia, L., Cruz, J.A., Lim, E., Sobsey, C.A., Shrivastava, S., Huang, P., Liu, P., Fang, L., Peng, J., Fradette, R., Cheng, D., Tzur, D., Clements, M., Lewis, A., De Souza, A., Zuniga, A., Dawe, M., Xiong, Y., Clive, D., Greiner, R., Nazyrova, A., Shaykhutdinov, R., Li, L., Vogel, H.J., and Forsythe, I., Nucleic Acids Res., 2009, vol. 37 (Database issue), pp. D603–610.

    Article  CAS  Google Scholar 

  37. Lin, L., Yu, Q., Yan, X., Hang, W., Zheng, J., Xing, J., and Huang, B., Analyst, 2010, vol. 135, pp. 2970–2978.

    Article  CAS  Google Scholar 

  38. Kaddurah-Daouk, R. and Krishnan, K.R., Neuropsychopharmacology, 2009, vol. 34, pp. 173–186.

    Article  CAS  Google Scholar 

  39. Lokhov, P.G., Kharybin, O.N., and Archakov, A.I., Int. J. Mass Spectr., 2012, vol. 309, pp. 200–205.

    CAS  Google Scholar 

  40. Lokhov, P.G., Dashtiev, M.I., Moshkovskii, S.A., and Archakov, A.I., Metabolomics, 2010, vol. 6, pp. 156–163.

    Article  CAS  Google Scholar 

  41. Sabatine, M.S., Liu, E., Morrow, D.A., Heller, E., McCarroll, R., Wiegand, R., Berriz, G.F., Roth, F.P., and Gerszten, R.E., Circulation, 2005, vol. 112, pp. 3868–3875.

    Article  CAS  Google Scholar 

  42. Wang, T.J., Larson, M.G., Vasan, R.S., Cheng, S., Rhee, E.P., McCabe, E., Lewis, G.D., Fox, C.S., Jacques, P.F., Fernandez, C., O’Donnell, C.J., Carr, S.A., Mootha, V.K., Florez, J.C., Souza, A., Melander, O., Clish, C.B., and Gerszten, R.E., Nat. Med., 2011, vol. 17, pp. 448–453.

    Article  Google Scholar 

  43. Odunsi, K., Wollman, R.M., Ambrosone, C.B., Hutson, A., McCann, S.E., Tammela, J., Geisler, J.P., Miller, G., Sellers, T., Cliby, W., Qian, F., Keitz, B., Intengan, M., Lele, S., and Alderfer, J.L., Int. J. Cancer, 2005, vol. 113, pp. 782–788.

    Article  CAS  Google Scholar 

  44. Sun, J., Shannon, M., Ando, Y., Schnackenberg, L.K., Khan, N.A., Portilla, D., and Beger, R.D., J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2012, vol. 893–894, pp. 107–113.

    Google Scholar 

  45. Gutiu, I.A., Andries, A., Radulescu, F., Georgescu, A.-M., and Cioaca, D., Rom. J. Intern. Med., 2010, vol. 48, pp. 187–191.

    CAS  Google Scholar 

  46. Kaddurah-Daouk, R., Kristal, B.S., and Weinshilboum, R.M., Annu. Rev. Pharmacol. Toxicol., 2008, vol. 48, pp. 653–683.

    Article  CAS  Google Scholar 

  47. Schnackenberg, L.K. and Beger, R.D., Toxicol. Mech. Methods, 2008, vol. 18, pp. 301–311.

    Article  CAS  Google Scholar 

  48. Lindon, J.C., Holmes, E., and Nicholson, J.K., Pharm. Res., 2006, vol. 23, pp. 1075–1088.

    Article  CAS  Google Scholar 

  49. Metz, C.E., Semin. Nucl. Med., 1978, vol. 8, pp. 283–298.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, Moscow, 119121, Russia

    O. P. Trifonova, P. G. Lokhov & A. I. Archakov

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  1. O. P. Trifonova
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  2. P. G. Lokhov
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  3. A. I. Archakov
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Correspondence to O. P. Trifonova.

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Original Russian Text © O.P. Trifonova, P.G. Lokhov, A.I. Archakov, 2013, published in Biomeditsinskaya Khimiya.

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Trifonova, O.P., Lokhov, P.G. & Archakov, A.I. Metabolic profiling of human blood. Biochem. Moscow Suppl. Ser. B 7, 179–186 (2013). https://doi.org/10.1134/S1990750813030128

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