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Inhibition of amyloid aggregation of bovine serum albumin by sodium dodecyl sulfate at submicellar concentrations

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Abstract

Sodium dodecyl sulfate (SDS), as an anionic surfactant, can induce protein conformational changes. Recent investigations demonstrated different effects of SDS on protein amyloid aggregation. In the present study, the effect of SDS on amyloid aggregation of bovine serum albumin (BSA) was evaluated. BSA transformed to β-sheet-rich amyloid aggregates upon incubation at pH 7.4 and 65°C, as demonstrated by thioflavin T fluorescence, circular dichroism, and transmission electron microscopy. SDS at submicellar concentrations inhibited BSA amyloid aggregation with IC50 of 47.5 μM. The inhibitory effects of structural analogs of SDS on amyloid aggregation of BSA were determined to explore the structure–activity relationship, with results suggesting that both anionic and alkyl moieties of SDS were critical, and that an alkyl moiety with chain length ≥10 carbon atoms was essential to amyloid inhibition. We attributed the inhibitory effect of SDS on BSA amyloid aggregation to interactions between the detergent molecule and the fatty acid binding sites on BSA. The bound SDS stabilized BSA, thereby inhibiting protein transformation to amyloid aggregates. This study reports for the first time that the inhibitory effect of SDS on albumin fibrillation is closely related to its alkyl structure. Moreover, the specific binding of SDS to albumin is the main driving force in amyloid inhibition. This study not only provides fresh insight into the role of SDS in amyloid aggregation of serum albumin, but also suggests rational design of novel antiamyloidogenic reagents based on specific-binding ligands.

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Abbreviations

BSA:

bovine serum albumin

CD:

circular dichroism

HSA:

human serum albumin

SDS:

sodium dodecyl sulfate

TEM:

transmission electron microscopy

ThT:

thioflavin T

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Shaanxi Normal University, Xi’an, 710119, China

    Xue-Jiao Ma, Yin-Juan Zhang & Cheng-Ming Zeng

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Correspondence to Cheng-Ming Zeng.

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Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM17-395, November 6, 2017.

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Ma, XJ., Zhang, YJ. & Zeng, CM. Inhibition of amyloid aggregation of bovine serum albumin by sodium dodecyl sulfate at submicellar concentrations. Biochemistry Moscow 83, 60–68 (2018). https://doi.org/10.1134/S000629791801008X

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