Abstract
Eight symbiotic mutants of pea (Pisum sativum L.) induced by chemical mutagenesis have been studied by the vegetative graft method in a “stem/root” manner. It has been found that symbiotic traits are controlled by the root in nonnodulating mutants K20a and K1005m, mutants with ineffective nodules K287 and K1a, and supernodulating mutants K10a and K12a. In contrast, in supernodulating mutants K301 and K22a, these traits are controlled by the stem. It is concluded that different mechanisms control supernodulation in supernodulating mutants K10a and K12a, on the one hand, and K301 and K22a, on the other.
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Abd-Alla, M.H., Regulation of nodule formation in soybean-Bradyrhizobium symbiosis is controlled by shoot or/and root signals, Plant Growth Regulation, 2001, vol. 34, no. 2, pp. 241–250.
Caetano-Anolles, G. and Bauer, W.D., Feedback regulation of nodule formation in alfalfa, Planta, 1988, vol. 175, pp. 546–557.
Caetano-Anolles, G. and Gresshoff, P.M., Plant genetic control of nodulation, Annu. Rev. Microbiol., 1991, vol. 45, pp. 345–382.
Chesnokov, V.A. and Bazyrina, E.N., Growing plants without soil in artificial media, Vestn. S-kh. Nauki, 1957, no. 4, pp. 121–128.
Delves, A.C., Mathews, A., Day, D.A., et al., Regulation of the soybean-Rhizobium nodule symbiosis by shoot and root factors, Plant Physiol., 1986, vol. 82, pp. 588–590.
Gresshoff, P.M., Molecular genetic analysis of nodulation genes in soybean, Plant Breed. Rev., 1993, vol. 11, pp. 275–318.
Hardy, R.W.F., Holsten, R.D., Jackson, E.K., et al., The acetylene-ethylene assay for N2-fixation: laboratory and field evaluation, Plant Physiol., 1968, vol. 43, no. 8, pp. 1185–1207.
Hirsch, A.M. and Fang, Y., Plant hormones and nodulation: what’s the connection?, Plant. Mol. Biol., 1994, vol. 26, pp. 5–9.
Ikeda, J., Analysis of regulation site and manner of abundant nodulation in a soybean (Glycine max (L.) Merr.) cultivar, Kitamusume, using grafting techniques, Soil Sci. Plant Nutr., 2001, vol. 47, no. 4, pp. 703–710.
Kholodar’, A.V., Sidorova, K.K., and Shumny, V.K., Phytohormone levels in different types of symbiotic mutants of pea, Russ. J. Genet., 2001, vol. 37, no. 11, pp. 1275–1278.
Kneen, B.E., Weeden, N.F., and LaRue, T.A., Nonnodulating mutants of Pisum sativum (L.) cv. Sparkle, J. Hered., 1994, vol. 85, no. 2, pp. 129–133.
LaRue, T.A. and Weeden, N.F., The symbiosis genes of pea, Pisum Genet., 1992, vol. 24, pp. 5–12.
LaRue, T.A., Temnykh, S., and Weeden, N.F., Sym18, a novel gene conditioning altered strain specificity in Pisum sativum cv. Sparkle, Plant Soil, 1996, vol. 180, no. 2, pp. 191–195.
Lee, S.H., Ashley, D.A., and Boerma, H.R., Regulation of nodule development in supernodulating mutants and wild-type soybean, Crop Sci., 1991, vol. 31, no. 3, pp. 688–693.
Li, D.X., Kinkema, M., and Gresshoff, P.M., Autoregulation of nodulation (AON) in Pisum sativum (pea) involves signaling events associated with both nodule primordia development and nitrogen fixation, J. Plant Physiol., 2009, vol. 166, no. 9, pp. 955–967.
Nutman, P.S., Nuclear and cytoplasmic inheritance of resistance to infection by nodule bacteria in red clover, Heredity, 1949, vol. 3, pp. 263–291.
Nutman, P.S., The influence of the legume in root-nodule symbiosis: a comparative study of host determinants and functions, Biol. Rev., 1956, vol. 31, pp. 109–151.
Pavlova, Z.B. and Lutova, L.A., Characterization of pea symbiotic mutants for traits related to hormonal status, Russ. J. Genet., 2000, vol. 36, no. 6, pp. 656–660.
Phillips, D.A., A cotyledonous inhibitor of root nodulation in Pisum sativum, Physiol. Plant., 1971, vol. 25, pp. 482–487.
Postma, J.G., Jacobsen, E., and Feenstra, W.J., Three pea mutants with an altered nodulation studied by genetic analysis and grafting, J. Plant Physiol., 1988, vol. 132, pp. 424–430.
Sagan, M. and Gresshoff, P.M., Kinetics of nodule development in pea cv. Frisson and in two supernodulating mutants, Abstr. of the 10th Intern. Congr. on Nitrogen Fixation, St. Petersburg, Russia, 1995, p. 289.
Sagan, M., Huguet, T., and Duc, G., Phenotypic characterization and classification of nodulation mutants of pea (Pisum sativum L.), Plant Sci., 1994, vol. 100, pp. 59–70.
Sagan, M. and Duc, G., Sym28 and Sym29, two new genes involved in regulation of nodulation in pea (Pisum sativum L.), Symbiosis, 1996, vol. 20, no. 3, pp. 229–245.
Sidorova, K.K. and Uzhintseva, L.P., The use of mutants to identify the genes controlling symbiotic characters in pea, Russ. J. Genet., 1992, vol. 28, no. 4, pp. 144–151.
Sidorova, K.K. and Uzhintseva, L.P., Localization of the mutant gene nod4 controlling supernodulation in pea, Dokl. Ross. Akad. Nauk, 1994, vol. 336, no. 6, pp. 847–849.
Sidorova, K.K. and Shumnyi, V.K., The new nodulation-controlling gene Nod5-nod5 in Peas (Pisum sativum L.), Dokl. Biol. Sci., 1997, vol. 353, pp. 190–192.
Sidorova, K.K., Shumny, V.K., Vlasova, E.Yu., et al., A new gene for supernodulation in pea Nod6, Pisum Genet., 2003, vol. 35, pp. 28–29.
Sidorova, K.K. and Shumnyi, V.K., A Collection of symbiotic mutants in pea Pisum sativum L.: creation and genetic study, Russ. J. Genet., 2003, vol. 39, no. 4, pp. 406–413.
Stougaard, J., Regulators and regulation of legume root nodule development, Plant Physiol., 2000, vol. 124, pp. 531–540.
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Original Russian Text © E.Yu. Vlasova, K.K. Sidorova, M.N. Glyanenko, T.M. Mischenko, 2012, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2012, Vol. 16, No. 4/2, pp. 879–886.
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Vlasova, E.Y., Sidorova, K.K., Glyanenko, M.N. et al. A study of symbiosis formation control in pea mutants by the vegetative graft method. Russ J Genet Appl Res 3, 191–196 (2013). https://doi.org/10.1134/S207905971303012X
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DOI: https://doi.org/10.1134/S207905971303012X