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Application of the immobilized bacterial recombinant lipase from Geobacillus stearothermophilus G3 for the production of fatty acid methyl esters

  • Biocatalysis
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Abstract

The heterogeneous BCL biocatalyst based on the recombinant extracellular lipase from the thermophilic bacteria Geobacillus stearothermophilus G3 with an activity of 23.6 U.A./g was prepared by covalent immobilization on aminated silica gel. The effect of the solvent, temperature (30–60°C), methanol : oil molar ratio (1 : 1 to 9 : 1), and the amounts of water (1–10%) and catalyst (0.25–25%) on the yield of fatty acid methyl esters (FAME) during the methanolysis of sunflower oil with BCL was studied. The maximum yield of FAME was 43%. The biocatalyst exhibits high operational stability: after 480 h of operation (20 cycles), it retains more than 50% of its original activity, making BCL a promising catalyst for application in manufacturing of FAME as feedstock for biodiesel production.

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Authors and Affiliations

  1. Boreskov Institute of Catalysis, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    Y. V. Samoylova, A. V. Piligaev, K. N. Sorokina & V. N. Parmon

  2. Federal Research Center Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    K. N. Sorokina, A. S. Rozanov & S. E. Peltek

  3. Novosibirsk State University, Novosibirsk, 630090, Russia

    K. N. Sorokina & V. N. Parmon

  4. Gubkin Russian State University of Oil and Gas, Moscow, 119991, Russia

    A. A. Novikov & N. R. Almyasheva

Authors
  1. Y. V. Samoylova
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  2. A. V. Piligaev
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  3. K. N. Sorokina
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  4. A. S. Rozanov
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  5. S. E. Peltek
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  6. A. A. Novikov
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  7. N. R. Almyasheva
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  8. V. N. Parmon
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Correspondence to Y. V. Samoylova.

Additional information

Original Russian Text © Y.V. Samoylova, A.V. Piligaev, K.N. Sorokina, A.S. Rozanov, S.E. Peltek, A.A. Novikov, N.R. Almyasheva, V.N. Parmon, 2015, published in Kataliz v Promyshlennosti.

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Samoylova, Y.V., Piligaev, A.V., Sorokina, K.N. et al. Application of the immobilized bacterial recombinant lipase from Geobacillus stearothermophilus G3 for the production of fatty acid methyl esters. Catal. Ind. 8, 187–193 (2016). https://doi.org/10.1134/S2070050416020082

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  • DOI: https://doi.org/10.1134/S2070050416020082

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