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Emergence of acetylcholine resistance and loss of rhythmic activity associated with the development of hypertension, obesity, and type 2 diabetes

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

The aim of this work was to study the influence of aging, obesity, metabolic syndrome (MS), hypertension (HT), and type 2 diabetes (T2D) on the endogenous rhythmic activity and the development acetylcholine resistance in aorta rings of male rats. T2D was produced by a free access to fat (lard). It was shown that phenylephrine (PE) or 5-hydroxytryptamine (5-HT) induces two types of rhythmic contractions: with periods T 1 = 3–10 s and T 2 = 50–70 s and amplitudes A 1 = 1–5% and A 2 = 20–40% of the maximal contraction force (F max), respectively. Such periodic modes can be caused by the operation of two known positive feedback loops (PFL) based on the Ca2+-induced activation of IP3 receptor (IP3R) or phospholipase C PFL1 and PFL2, respectively, and are not eliminated by L-NAME. Slow rhythmic activity induced by acetylcholine (Ach) with period T 3 = 7–20 min and amplitude A 3 = 20–30% of F max was observed only in young animals (under 6 months) and can be determined by the operation of PFL3, involving Ca2+, NO, kinase G, cADP-ribose, and the ryanodine receptor (RyR). Fast mode of contractions (T 1, A 1) is maintained regardless of age and the presence of MS and HT (140 mm Hg and higher) and disappears only at later stages of the T2D development. Probability of intermediate mode of contractions (T 2, A 2) decreases to 0.20–0.25 at the age of 14–16 months or during the development of HT and MS. In these circumstances, Ach could cause relaxation of preconstricted rings only to 40 and 60% of F max, respectively. At the stages of the T2D development characterized by high values of arterial pressure (above 150 mm Hg) and of the glucose (10–12 mM), ammonium (120–180 μM), and blood lipid levels, as well as by liver dysfunction (fibrosis/cirrhosis), the rhythmic activity of any type is lost and dysfunction of the initial part of the signaling cascade with the participation of PFL3 is manifested by the absence of responses to Ach or L-NAME. Coenzyme NAD (agonist of the P2Y receptors, К+ channel activator and a precursor of cADP-ribose) can exert a partial relaxation of aorta rings from healthy animals and animals with MS. Nicotinamide (product and an inhibitor of ADP-ribosyl cyclase) and SNP (donor of NO) produce an effective relaxation of aorta rings from healthy animals and animals with T2D. Relaxing effect of nicotinamide may suggest a tandem operation of IP3R and RyR in the control of intracellular Ca2+ stores in vascular cells.

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Abbreviations

MS:

metabolic syndrome

T2D:

type 2 diabetes

HT:

vascular hypertension

BP:

arterial blood pressure

Ach:

acetylcholine

PE:

phenylephrine

5-HT:

5-hydroxytryptamine

CD38:

ADP-ribosyl cyclase

NAM:

nicotinamide

L-NAME:

an inhibitor of the endothelial NO-synthase

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Authors and Affiliations

  1. Institute of Cell Biophysics, Russian Academy of Sciences, Institutskaya ul. 3, Pushchino, Moscow oblast, 142290, Russia

    L. A. Andreeva & A. I. Sergeev

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya ul. 3, Pushchino, Moscow oblast, 142290, Russia

    E. V. Grishina & V. V. Dynnik

  3. Pushchino Branch of the Shemiakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow oblast, 142290, Russia

    A. V. Lobanov, G. A. Slastcheva & V. A. Rykov

  4. South Federal University, Bolshaya Sadovaya ul. 105/42, Rostov-on-Don, 344006, Russia

    A. V. Temyakov

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  1. L. A. Andreeva
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  2. E. V. Grishina
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  3. A. I. Sergeev
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  4. A. V. Lobanov
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  5. G. A. Slastcheva
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  6. V. A. Rykov
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  7. A. V. Temyakov
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  8. V. V. Dynnik
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Correspondence to V. V. Dynnik.

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Original Russian Text © L.A. Andreeva, E.V. Grishina, A.I. Sergeev, A.V. Lobanov, G.A. Slastcheva, V.A. Rykov, A.V. Temyakov, V.V. Dynnik, 2016, published in Biologicheskie Membrany, 2016, Vol. 33, No. 3, pp. 213–222.

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Andreeva, L.A., Grishina, E.V., Sergeev, A.I. et al. Emergence of acetylcholine resistance and loss of rhythmic activity associated with the development of hypertension, obesity, and type 2 diabetes. Biochem. Moscow Suppl. Ser. A 10, 199–206 (2016). https://doi.org/10.1134/S1990747816020033

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