Abstract
Platelets, specialized blood cells, are the most important members of the hemostatic system. Upon activation they divide into two subpopulations, differing greatly in their properties. Platelets from one of these subpopulations express phosphatidylserine (PS) on the outer layer of their membrane and retain secreted alpha-granule proteins on their surface. We inquired if transglutaminases participate in regulation of the formation of platelet subpopulations. Synthetic peptides F11KA and T26 inhibiting factor XIIIa and tissue transglutaminase, respectively, dansylcadaverine, and competitive inhibitors/substrates (serotonin, cystamine, and GTP) reduced the amount of PS-positive platelets. However, inhibitory antibody against tissue transglutaminase did not alter the number of PS-positive platelets, and influence of pan-transglutaminase inhibitor T101 was moderate (20% for the activation by thrombin with collagen-related peptide (CRP)) and statistically insignificant (p = 0.08). The addition of transglutaminases caused a statistically significant increase in the number of PS-positive platelets (by 27% and 14% for tissue transglutaminase and factor XIIIa, respectively) upon activation with CRP but not with thrombin. Taking into account a well-known non-specificity of most inhibitors used in this study and their high effective concentrations, we suggest that transglutaminases may significantly influence the formation of procoagulant platelets in some conditions (upon platelet stimulation via collagen receptor GPVI). However, our data do not support the idea that transglutaminases can play a major and universal role in this phenomenon.
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Original Russian Text © Ya.N. Kotova, A.A. Abaeva, V.N. Kolyadko, A.O. Yakimenko, F.I. Ataullakhanov, M.A. Panteleev, 2015, published in Biologicheskie Membrany, 2015, Vol. 32, No. 4, pp. 245–252.
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Kotova, Y.N., Abaeva, A.A., Kolyadko, V.N. et al. The role of transglutaminases in the regulation of phosphatidylserine-positive platelet formation. Biochem. Moscow Suppl. Ser. A 9, 229–235 (2015). https://doi.org/10.1134/S1990747815040054
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DOI: https://doi.org/10.1134/S1990747815040054