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Secretion and hydrolysis of ATP by Ehrlich ascites tumor cells during centrifugation. Dependence on calcium and temperature

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Precipitation of Ehrlich ascites tumor cells (EATC) by centrifugation causes ATP secretion. ATP secretion is accompanied by an increase of calcium concentration in the cytosol and persists for a long time (minutes) after centrifugation during the storage of cells at a low temperature. During prolonged storage (for more than 1.5 h), the concentration of extracellular ATP decreases to the level of ∼100 nM due to termination of secretion and ATP hydrolysis by surface ATPases. The rate of ATP hydrolysis exponentially falls with the temperature decrease from 36 to 8°C. ARL67156, a selective inhibitor of E-NTPDase-1, effectively suppresses the extracellular ATP hydrolysis. The intensity of ATP secretion does not correlate with the calcium ions concentration in the cytosol and Ca2+ mobilization from endoplasmic reticulum but correlates with the intensity of Ca2+ influx into the cells. The temperature dependences of ATP secretion intensity and Ca2+ entry rate coincide.

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Abbreviations

EATC:

Ehrlich ascites tumor cells

[Ca2+]i :

concentration of free Ca ions in the cytosol

ER:

endoplasmic reticulum

R24571:

calmidazolium

ARL67156:

ectonucleotidase1 (E-NTPDase-1) inhibitor

SOC:

channels, store-operated calcium channels

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Authors and Affiliations

  1. Institute of Cell Biophysics, Russian Academy of Science, Pushchino, Moscow oblast, 142290, Russia

    A. M. Krasnyi, A. I. Sergeev, L. P. Dolgacheva, E. L. Nikiforov & V. P. Zinchenko

  2. Pushchino State University, Pushchino, Moscow oblast, 142290, Russia

    A. I. Sergeev

Authors
  1. A. M. Krasnyi
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  2. A. I. Sergeev
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  3. L. P. Dolgacheva
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  4. E. L. Nikiforov
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  5. V. P. Zinchenko
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Correspondence to L. P. Dolgacheva.

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Original Russian Text © A.M. Krasnyi, A.I. Sergeev, L.P. Dolgacheva, E.L. Nikiforov, V.P. Zinchenko, 2010, published in Biologicheskie Membrany, 2010, Vol. 27, No. 1, pp. 106–113.

The article was translated by the authors.

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Krasnyi, A.M., Sergeev, A.I., Dolgacheva, L.P. et al. Secretion and hydrolysis of ATP by Ehrlich ascites tumor cells during centrifugation. Dependence on calcium and temperature. Biochem. Moscow Suppl. Ser. A 4, 90–96 (2010). https://doi.org/10.1134/S1990747810010149

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  • DOI: https://doi.org/10.1134/S1990747810010149

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