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Thiol antioxidants increase the intracellular level of reactive oxygen species and prolifetaion of SP2/0 mouse myeloma cells in serum-free medium

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Abstract

The connections between the presence of low molecular weight RSH-antioxidants (N-acetylcysteine, glutathione) in serum-free medium, generation of reactive oxygen species (ROS), and proliferation of SP2/0-SF mouse myeloma cells have been demonstrated. It is shown that the presence of RSH compounds in the medium within the studied range of concentrations changed the contents of ROS in cells and had a dose-dependent effect on cell proliferation. Stimulation of the proliferative activity did not depend on the nature of an RSH compound. The optimal concentration for the both antioxidants was 0.2 mM. A further increase of the concentration led to inhibition of cell proliferation to different degrees for N-acetylcysteine and glutathione.

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Abbreviations

ROS:

reactive oxygen species

GSH:

reduced glutathione

NAC:

N-acetylcysteine

RSH:

thiols

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Authors and Affiliations

  1. Medical-Genetic Center, Moscow, 115478, Russia

    V. Yu. Tabakov, N. N. Veiko, V. V. Chestkov & S. V. Kostyuk

  2. OOO PanEco Research and Production Company, Moscow, 115522, Russia

    V. Yu. Tabakov & V. V. Chestkov

Authors
  1. V. Yu. Tabakov
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  2. N. N. Veiko
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  3. V. V. Chestkov
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  4. S. V. Kostyuk
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Correspondence to V. Yu. Tabakov.

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Original Russian Text © V.Yu. Tabakov, N.N. Veiko, V.V. Chestkov, S.V. Kostyuk, 2016, published in Tsitologiya, 2016, Vol. 58, No. 12, pp. 924–929.

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Tabakov, V.Y., Veiko, N.N., Chestkov, V.V. et al. Thiol antioxidants increase the intracellular level of reactive oxygen species and prolifetaion of SP2/0 mouse myeloma cells in serum-free medium. Cell Tiss. Biol. 11, 155–160 (2017). https://doi.org/10.1134/S1990519X17020067

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  • DOI: https://doi.org/10.1134/S1990519X17020067

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