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Effects of styryl dye RH 421 on the activity of amphotericin B in cell and model membranes

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Abstract

The combined action of polyene macrolid antibiotic amphotericin B and styryl dye RH 421 in model lipid membranes and in yeast Saccharomyces cerevisiae is studied. Addition of RH 421 to ergosterol-containing lipid bilayers leads to an increase by 8.5 ±3.2 times of the equilibrium number of open amphotericin channels. The agar-diffusion method shows that RH 421 enhances the antimicrobial effect of amphotericin B, leading to an increase by 1.5 times of the zone of growth inhibition of S. cerevisiae strain. Our data suggest that RH 421 is a potential synergist of amphotericin B and can be used in novel drugs with improved pharmacological properties.

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Abbreviations

AB:

amphotericin B

DMSO:

dimethylsulfoxide

DPPCh:

1,2-diphytanoyl-sn-glycero-3-phosphocholine

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

    E. V. Mikhailova, S. S. Efimova & O. S. Ostroumova

Authors
  1. E. V. Mikhailova
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  2. S. S. Efimova
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  3. O. S. Ostroumova
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Correspondence to E. V. Mikhailova.

Additional information

Original Russian Text © E.V. Mikhailova, S.S. Efimova, O.S. Ostroumova, 2013, published in Tsitologiya, Vol. 55, No. 2, 2013, pp. 136–139.

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Mikhailova, E.V., Efimova, S.S. & Ostroumova, O.S. Effects of styryl dye RH 421 on the activity of amphotericin B in cell and model membranes. Cell Tiss. Biol. 7, 289–292 (2013). https://doi.org/10.1134/S1990519X13030097

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  • DOI: https://doi.org/10.1134/S1990519X13030097

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