Abstract
Bone marrow multipotent mesenchymal stromal cells show considerable promise for the engineering of human three-dimensional transplants of cartilage tissue. We demonstrated the directed differentiation of BM MMSC in cells of cartilage tissue by culturing them in OPLA polymer three-dimensional scaffolds in a medium with chondrogenesis inducers. Cells were loaded into porous scaffolds by saturating polymer blocks with a cellular suspension. This was followed by high-speed centrifugation of the matrix-embedded cells and cultivation of the engineering constructs in a condrogenic medium for 28 days. Histological analysis of the three-dimensional transplants derived in vitro showed a uniform distribution of cells in the matrix. Morphologically, the cells were similar to the chondrocyte-like cells of articular cartilage. Immunohistochemical analysis revealed aggrecan and collagen type 2, which are the major markers of chondrogenesis, in the constructs. Preclinical research on immunodeficient mice showed that the engineering transplants were not toxic.
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Abbreviations
- AG:
-
antigens
- AB:
-
antibodies
- DMEM:
-
Eagle modified Dulbecco medium
- BM:
-
bone marrow
- MMSC:
-
multipotent mesenchymal stromal cells
- FCS:
-
fetal calf serum
- PE:
-
phycoerythrin
- CD:
-
locus of cytodifferentiation
- PBS:
-
phosphate buffer
- TGF:
-
transforming growth factor
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Original Russian Text © A.S. Teplyashin, S.V. Korjikova, S.Z. Sharifullina, M.S. Rostovskaya, N.I. Chupikova, N.Yu. Vasyunina, N.V. Andronova, E.M. Treshalina, I.P. Savchenkova, 2007, published in Tsitologiya, Vol. 49, No. 2, 2007.
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Teplyashin, A.S., Korjikova, S.V., Sharifullina, S.Z. et al. Differentiation of multipotent mesenchymal stromal cells of human bone marrow into cells of cartilage tissue by culturing in three-dimensional OPLA scaffolds. Cell Tiss. Biol. 1, 125–132 (2007). https://doi.org/10.1134/S1990519X07020034
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DOI: https://doi.org/10.1134/S1990519X07020034