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The effect of N-arachidonoyldopamine on the dynamics of the intracellular calcium concentration in hippocampal neurons in the model of postischemic epileptogenesis in vitro

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Abstract

We studied the dynamics of the intracellular concentration of calcium ions ([Ca2+] i ) and the influence of the endogenous cannabinoid N-arachidonoyldopamine (N-ADA) on disturbances of calcium homeostasis in cultured hippocampal neurons in the model of postischemic epileptogenesis (PE) in vitro, in accordance with a previously published method. It was found that 24 h after treatment with 20 μM glutamate, its application at a concentration of 50 μM results in a persistent increase in [Ca2+] i whereas in neurons that were not previously subjected to glutamate treatment an increase in [Ca2+] i after the application of 50 μM glutamate was reversible. The presence of N-ADA (5 μM) in the incubation medium both simultaneously with 20 μM glutamate exposure and for 24 h after it promoted recovery of the [Ca2+] i level to the initial level. The results indicate that application of N-ADA promotes normalization of neuronal calcium homeostasis in a PE model in vitro.

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Authors and Affiliations

  1. Research Center of Neurology, Moscow, Russia

    S. N. Kolbaev, V. V. Simonova, M. Yu. Bobrov, I. N. Sharonova & L. G. Khaspekov

  2. per. Obukha 5, Moscow, 105064, Russia

    S. N. Kolbaev

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  1. S. N. Kolbaev
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  2. V. V. Simonova
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  3. M. Yu. Bobrov
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  4. I. N. Sharonova
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  5. L. G. Khaspekov
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Correspondence to S. N. Kolbaev.

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Original Russian Text © S.N. Kolbaev, V.V. Simonova, M.Yu. Bobrov, I.N. Sharonova, L.G. Khaspekov, 2016, published in Neirokhimiya, 2016, Vol. 33, No. 4, pp. 337᾿43.

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Kolbaev, S.N., Simonova, V.V., Bobrov, M.Y. et al. The effect of N-arachidonoyldopamine on the dynamics of the intracellular calcium concentration in hippocampal neurons in the model of postischemic epileptogenesis in vitro. Neurochem. J. 10, 312–317 (2016). https://doi.org/10.1134/S1819712416040061

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  • DOI: https://doi.org/10.1134/S1819712416040061

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