Abstract
We studied the thermal denaturation of acetylcholinesterase of brain synaptic membranes of ground squirrels (Spermophylus pigmaeus) in hibernation. We found that the enzyme activity increases during bouts of hibernation and decreases during interbout arousal. The kinetics of the enzyme denaturation at 48°C has two exponential stages, fast and slow, with rate constants of k 1 = 0.376 min−1 and k 2 = 0.040 min−1, respectively (for control animals in the summer period). The rate constants of denaturation decreased during bouts of deep hibernation and interbout arousal, as compared to the summer control. We discuss the possible mechanisms of changes in the enzymatic activity and thermostability of brain acetylcholinesterase during hibernation.
Similar content being viewed by others
References
Kalabukhov, N.I., Spyachka mlekopitayushchikh (Hibernation of Mammals), Moscow: Nauka, 1985.
Wang, L.C.H and Lee, N.F, in Handbook of Physiology, Fregly, M.J. and Blatteis, C.M., Eds., New York: Oxford University Press, 1996, pp. 507–531.
Kayzer, Ch., The Physiology of Natural Hibernation, New York: Pergamon Press, 1961.
Buck, C.L. and Barnes, B.M., Am. J. Physiol., 2000, vol. 279, pp. 255–262.
Carey, H.V., Andrews, M.T., and Martin, M.T., Physiol. Rev., 2003, vol. 83, pp. 1153–1181.
Storey, K.B. and Storey, J.M., Biol. Rev., 2004, vol. 79, pp. 207–233.
Sarter, M. and Bruno, J.P., Brain Res. Rev., 1997, vol. 23, pp. 28–46.
Dani, J.A. and Bertrand, D., Ann. Rev. Pharmacol. Toxicol., 2007, vol. 47, pp. 699–729.
Chou, T.C., Rotman, S.R., and Saper, C.B., Neurosci. Let, 2004, vol. 370, pp. 123–126.
Klichkhanov, N.K., Khalilov, R.A., Meilanov, I.S., and Avshalumov, M.V., Biol. Membrany, 2001, vol. 18, no. 2, pp. 111–119.
Dzhafarova, A.M., Meilanov, I.S., and Klichkhanov, N.K., Problemy i perspektivy sovremennoi nauki, Il’inskikh, N.N., Ed., Tomsk, 2008.
Lyubarev, A.E. and Kurganov, B.I., Usp. Biol. Khim., 2000, vol. 40, pp. 43–84.
Hajos, F., Brain Res., 1975, vol. 93, pp. 484–489.
Ellman, J.L., Courtney, K.D., Andres, V.J., and Featherstone, R.M., Biochem. Pharmacol., 1961, vol. 7, pp. 88–95.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., J. Biol. Chem., 1951, vol. 193, pp. 265–275.
Kantor, Ch. and Shimmel, P., in Biofizicheskaya khimiya (Biophysical Chemistry), Moscow: Mir, 1985.
Maloletkina, O.I., Markossian, K.A., Belousova, L.V., Kleimenov, S.Yu., Orlov, V.N., Makeeva, V.F., and Kurganov, B.I., Int. J. Biol. Macromol., 2010.
Zimmerman, J. and Soreq, H., Cell Tissue Res., 2006, vol. 326, pp. 655–669.
Bourne, Y., Taylor, P., Bougis, P.E., and Marchot, P., J. Biol. Chem., 1999, vol. 274, pp. 2963–2970.
Bourne, Y., Grassi, J., Bougis, P.E., and Marchot, P., J. Biol. Chem., 1999, vol. 274, pp. 30370–30376.
Gorfe, A.A., Lu, B., Yu, Z., and MacCammon, J.A, Biophys. J., 2009, vol. 97, pp. 897–905.
Perrier, A.L., Massoulie, J., and Krejci, E., Neuron, 2002, vol. 33, pp. 275–285.
Bloj, B., Galo, M., Morero, R.D., and Faras, R.N., J. Nutr., 1979, vol. 109, pp. 63–69.
Logue, J., De Vries, A., Fodor, E., and Cossins, A., J. Exp. Biol, 2000, vol. 203, pp. 2105–2115.
Wütrich, K., Wagner, G., Richarz, R., and Braun, W., Biophys. J., 1980, vol. 32, no. 1, pp. 549–560.
Grifman, M., Arbela A., Ginzberg D., Glick D., Elgavish S., Shaanan B., Soreq H, Mol. Brain Res., 1997, vol. 51, pp. 179–187.
Zimmermann, H., Neurochem. Int., 2008, vol. 52, pp. 634–648.
Pawlowska, Z., Hogan, M.V., Kornecki, E., and Ehrlich, Y.H., J. Neurochem., 1993, vol. 60, pp. 678–686.
Zimmermann, H., Drug Dev. Res, 1996, vol. 39, pp. 337–352.
Lendvai, B. and Vizi, E.S., Physiol. Rev., 2008, vol. 88, pp. 333–349.
Nishiyama, H. and Linden, D.J., Neurosci, 2007, vol. 10, pp. 675–677.
Beckman, A.L., Satinoff, E., and Stanton, T.L., Am. J. Physiol., 1976, vol. 230, pp. 368–375.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © I.S. Meilanov, N.K. Klichkhanov, A.M. Djafarova, M.H. Tikra, 2010, published in Neirokhimiya, 2010, Vol. 27, No. 4, pp. 280–285.
Rights and permissions
About this article
Cite this article
Meilanov, I.S., Klichkhanov, N.K., Djafarova, A.M. et al. Thermal denaturation of acetylcholinesterase of brain synaptic membranes of ground squirrels in hibernation. Neurochem. J. 4, 252–256 (2010). https://doi.org/10.1134/S1819712410040021
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1819712410040021