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Effects of GABAA-ergic ligands on Cl− transport induced by the Cl−, HCO −3 -ATPase from carp (Cyprinus carpio L.) brain reconstituted in proteoliposomes

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Abstract

A Cl−, HCO −3 -ATPase isolated from the plasma membranes of fish brain was reconstituted in artificial proteoliposomes. The original sensitivity to GABAA-ergic ligands was preserved in the reconstituted enzyme. GABA was shown to activate the liposome-embedded enzyme in a concentration range from 10 to 100 μM while picrotoxin (100 μM) removed this activating affect. A Cl−-sensitive fluorescent probe (MEQ) was used to study the ATP-dependent Cl− transport through the membrane of the proteoliposomes. ATP (in concentrations of 1.5 mM and higher) was found to induce Cl−-transport into the proteoliposomes. This ATP-dependent transport was increased in the presence of GABA (100 μM), while the latter effect could also be blocked by picrotoxin (100 μM). It was concluded that the Cl−, HCO −3 -ATPase could be involved in ATP-dependent Cl−-transport and regulated by activators and blockers of GABAA receptors.

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Abbreviations

GABA:

γ-aminobutyric acid

PMSF:

phenylmethylsulfonyl fluoride

MEQ:

6-methoxy-N-ethylquinolinium iodide

TCA:

trichloroacetic acid

References

  1. Gerencser, G.A., Crit. Rev. Biochem. Mol. Biol., 1996, vol. 31, pp. 303–307.

    PubMed  CAS  Google Scholar 

  2. Inagaki, C., Hara, M., and Zeng, X.T., Exp. Zool., 1996, vol. 275, pp. 262–268.

    Article  CAS  Google Scholar 

  3. Ikeda, M. and Schmid, R., Biochemistry, 1990, vol. 29, no. 8, pp. 2057–2065.

    Article  PubMed  CAS  Google Scholar 

  4. Menzikov, S.A. and Menzikova, O.V., Biokhimiya, 2002, vol. 67, no. 2, pp. 278–282.

    Google Scholar 

  5. Menzikov, S.A. and Menzikova, O.V., Dokl. Akad. Nauk, 2002, vol. 382, no. 6, pp. 833–835.

    Google Scholar 

  6. Menzikov, S.A. and Menzikova, O.V., Ukr. Biokhim. Zh., 2004, vol. 76, no. 2, pp. 53–58.

    PubMed  CAS  Google Scholar 

  7. Menzikov, S.A. and Menzikova, O.V., Izv. Ross. Akad. Nauk, Ser. Biol., 2006, no. 2, pp. 142–147.

  8. Menzikov, S.A. and Menzikova, O.V., Dokl. Akad. Nauk, 2006, vol. 407, no. 2, pp. 263–266.

    Google Scholar 

  9. Stekhoven, F.S. and Bonting, S.L., Physiol. Rev., 1981, vol. 61, pp. 1–76.

    CAS  Google Scholar 

  10. Severin, S.E. and Solov’eva, S.A. Praktikum po biokhimii (Practical Work in Biochemistry), Moscow: MGU, 1989.

    Google Scholar 

  11. Chen, P.S., Toribara, T.Y., and Warner, H., Anal. Chem., 1956, vol. 28, pp. 1756–1758.

    Article  CAS  Google Scholar 

  12. Bradford, M.M., Anal. Biochem., 1976, vol. 72, pp. 248–254.

    Article  PubMed  CAS  Google Scholar 

  13. Zeng, X.-T., Hara, M., and Inagaki, C., Brain Res., 1994, vol. 641, pp. 167–170.

    Article  PubMed  CAS  Google Scholar 

  14. Biwersi, J. and Verkman, A.S., Biochemistry, 1991, vol. 30, no. 32, pp. 7879–7883.

    Article  PubMed  CAS  Google Scholar 

  15. Dunn, S.M.J., Martin, C.R., Agey, M.W., and Miyazaki, R., Biochemistry, 1989, vol. 28, pp. 2545–2551.

    Article  PubMed  CAS  Google Scholar 

  16. Inglefield, J.R. and Schwartz-Bloom R.D., Methods: A companion to Methods in Enzymology, 1999, vol. 18, pp. 197–203.

    Article  PubMed  CAS  Google Scholar 

  17. Menzikov, S.A. and Menzikova, O.V., Neurosci. Lett., 2002, vol. 334, pp. 161–164.

    Article  PubMed  CAS  Google Scholar 

  18. Yagyu, K., Kitagawa, K., Irie, T., et al., Brain Res., 1999, vol. 821, pp. 530–534.

    Article  PubMed  CAS  Google Scholar 

  19. Bettendorff, L., Lakaye, B., Margineanu, I., et al., J. Neurochem., 2002, vol. 81, no 4, pp. 792–801.

    Article  PubMed  CAS  Google Scholar 

  20. Eshleman, A.J. and Murray, T.F., Neuropharmacol., 1991, vol. 30, pp. 1333–1341.

    Article  CAS  Google Scholar 

  21. Hyden, H., Rapallino, M.V., and Cupello, A., Prog. Neurobiol., 2000, vol. 60, pp. 471–499.

    Article  PubMed  CAS  Google Scholar 

  22. Kaur, G., Sharma, P., and Bhardwaj, S., Mol. Cell. Biochem., 1997, vol. 167, no. 1–2, pp. 107–111.

    Article  PubMed  CAS  Google Scholar 

  23. Gerasimenko, V.G., Danilenko, M.P., Zarkeshev, E.G., and Esyrev, O.V., Ukr. Biokhim. Zh., 1991, vol. 63, no. 1, pp. 44–50.

    PubMed  CAS  Google Scholar 

  24. Kometiani, Z.P. and Tsakadze, L.G., Biol. Nauki, 1990, no. 3, pp. 36–42.

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Authors and Affiliations

  1. Institute of General Pathology and Pathological Physiology, Russian Academy of Medical Sciences, ul. Baltiiskaya 8, Moscow, 125315, Russia

    S. A. Menzikov & O. V. Menzikova

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  1. S. A. Menzikov
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  2. O. V. Menzikova
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Correspondence to S. A. Menzikov.

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Original Russian Text © S.A. Menzikov, O.V. Menzikova, 2007, published in Neirokhimiya, 2006, Vol. 23, No. 2, pp. 106–111.

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Menzikov, S.A., Menzikova, O.V. Effects of GABAA-ergic ligands on Cl− transport induced by the Cl−, HCO −3 -ATPase from carp (Cyprinus carpio L.) brain reconstituted in proteoliposomes. Neurochem. J. 1, 31–36 (2007). https://doi.org/10.1134/S1819712407010047

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  • DOI: https://doi.org/10.1134/S1819712407010047

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