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Development of target delivery system based on actinomycin class drugs and recombinant alpha-fetoprotein

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

A recombinant alpha-fetoprotein (rAFP) was obtained in the yeast P. pastoris system, and its functional activity was confirmed. A method for producing polymer particles loaded with dactinomycin was developed, and a conjugate of these nanoparticles with rAFP was synthesized. The efficiency of the obtained conjugate on the HeLa, SKOV3, and MG-63 tumor cells and the absence of toxicity on the normal cells was shown. Experiments in vivo demonstrated a significant increase in the antitumor efficacy of the conjugate at a lower general toxicity as compared to the commercially available dactinomycin.

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Authors and Affiliations

  1. Russian Research Center for Molecular Diagnostics and Therapy, Moscow, 117149, Russia

    E. D. Nikolskaya, O. A. Zhunina, N. G. Yabbarov, B. I. Krugliy & E. S. Severin

  2. Moscow State University of Fine Chemical Technologies (MITHT), Moscow, 119571, Russia

    V. I. Shvets

Authors
  1. E. D. Nikolskaya
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  2. O. A. Zhunina
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  3. N. G. Yabbarov
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  4. V. I. Shvets
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  5. B. I. Krugliy
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  6. E. S. Severin
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Corresponding author

Correspondence to E. D. Nikolskaya.

Additional information

Original Russian Text © E.D. Nikolskaya, O.A. Zhunina, N.G. Yabbarov, V.I. Shvets, B.I. Krugliy, E.S. Severin, 2017, published in Doklady Akademii Nauk, 2017, Vol. 473, No. 6, pp. 739–741.

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Nikolskaya, E.D., Zhunina, O.A., Yabbarov, N.G. et al. Development of target delivery system based on actinomycin class drugs and recombinant alpha-fetoprotein. Dokl Biochem Biophys 473, 148–150 (2017). https://doi.org/10.1134/S1607672917020156

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  • DOI: https://doi.org/10.1134/S1607672917020156

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