Skip to main content
SpringerLink
Log in

Biocatalytic properties of immobilized inulinase

  • Published:
Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract

A heterogeneous biocatalyst based on inulinase immobilized on a nonionic sorbent of Stirosorb series was proposed. Thermal and acid inactivation of free and immobilized inulinase was examined and the corresponding inactivation constants were calculated. An increase in the thermal stability of the immobilized enzyme in comparison to the free one was found. The possibility of using the immobilized enzyme in the hydrolysis of inulin for ten cycles was determined.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Korneeva, O.S., Karbogidrazy: preparativnoe poluchenie, struktura i mekhanizm deistviya na oligo- i polisakharidy (Carbohydrases: Preparative Preparation, Structure, and Mechanism of Action on Oligo- and Polysaccharides), Voronezh, 2001.

    Google Scholar 

  2. Berezin, I.V., Issledovaniya v oblasti fermentativnogo kataliza i inzhenernoi enzimologii (Studies in Enzymatic Catalysis and Engineering Enzymology), Moscow, 1990.

    Google Scholar 

  3. Immobilizovannye kletki i fermenty (Immobilized Cells and Enzymes), Woodward, J., Ed., Moscow, 1988.

  4. Shkutina, I.V., Stoyanova, O.F., and Selemenev, V.F., Khim. Rastit. Syr’ya, 2012, no. 2, pp. 27–31.

    Google Scholar 

  5. Davankov, V.A. and Tsyurupa, M.P., Reactive Polymers, 2002, vol. 53, no. 1, pp. 193–203.

    Google Scholar 

  6. Chibata, I., Pure Appl. Chem., 1978, vol. 50, no. 7, pp. 667–675.

    Article  CAS  Google Scholar 

  7. Nakamura, T. and Nakatsu, S., J. Agr. Chem. Loc. Jap., 1997, vol. 1, no. 12, pp. 681–689.

    Google Scholar 

  8. Zholi, M., Fizicheskaya khimiya denaturatsii belkov (Physical Chemistry of Protein Denaturation), Moscow, 1968.

    Google Scholar 

  9. Putnam, F., Denaturatsiya belkov (Protein Denaturation), Moscow, 1984.

    Google Scholar 

  10. Varfolomeev, S.D. and Gurevich, K.G., Biokinetika: prakticheskii kurs (Biokinetics: Practical Course), Moscow, 1999.

    Google Scholar 

  11. Shkutina, I.V., Stoyanova, O.F., Selemenev, V.F., and Grigor’eva, G.A., Zh. Fiz. Khim., 2001, vol. 75, no. 12, pp. 2292–2293.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Voronezh State University, Voronezh, 394006, Russia

    I. V. Shkutina, O. F. Stoyanova & V. F. Selemenev

Authors
  1. I. V. Shkutina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. F. Stoyanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. F. Selemenev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. V. Shkutina.

Additional information

Original Russian Text © I.V. Shkutina, O.F. Stoyanova, V.F. Selemenev, 2015, published in Khimiya Rastitel’nogo Syr’ya, 2015, No. 1, pp. 141–145.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shkutina, I.V., Stoyanova, O.F. & Selemenev, V.F. Biocatalytic properties of immobilized inulinase. Russ J Bioorg Chem 42, 748–751 (2016). https://doi.org/10.1134/S1068162016070153

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1068162016070153

Keywords

Search

Navigation