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Investigation of the interaction of dimethyl sulfoxide with lipid membranes by small-angle neutron scattering

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Abstract

The influence of dimethyl sulfoxide (CH3)2SO (DMSO) on the structure of membranes of 1,2-dimiristoyl-sn-glycero-3-phosphatidylcholine (DMPC) in an excess of a water-DMSO solvent is investigated over a wide range of DMSO molar concentrations 0.0 ≤ X DMSO ≤ 1.0 at temperatures T = 12.5 and 55°C. The dependences of the repeat distance d of multilamellar membranes and the thickness d b of single vesicles on the molar concentration X DMSO in the L β’ gel and L α liquid-crystalline phases are determined by small-angle neutron scattering. The intermembrane distance d s is determined from the repeat distance d and the membrane thickness d b. It is shown that an increase in the molar concentration X DMSO leads to a considerable decrease in the intermembrane distance and that, at X DMSO = 0.4, the neighboring membranes are virtually in steric contact with each other. The use of the deuterated phospholipid (DMSO-D6) and the contrast variation method makes it possible, for the first time, to determine the number of DMSO molecules strongly bound to the membrane.

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Authors and Affiliations

  1. Joint Institute for Nuclear Research, ul. Zholio Kyuri 6, Dubna, Moscow oblast, 141980, Russia

    J. E. Gorshkova & V. I. Gordeliy

  2. Moscow Institute of Physics and Technology, Institutskiĭ per. 9, Dolgoprudnyĭ, Moscow oblast, 141700, Russia

    V. I. Gordeliy

  3. Research Centre Jüelich, Jüelich, D-52425, Germany

    V. I. Gordeliy

Authors
  1. J. E. Gorshkova
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  2. V. I. Gordeliy
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Original Russian Text © J.E. Gorshkova, V.I. Gordeliy, 2007, published in Kristallografiya, 2007, Vol. 52, No. 3, pp. 560–563.

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Gorshkova, J.E., Gordeliy, V.I. Investigation of the interaction of dimethyl sulfoxide with lipid membranes by small-angle neutron scattering. Crystallogr. Rep. 52, 535–539 (2007). https://doi.org/10.1134/S1063774507030364

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  • DOI: https://doi.org/10.1134/S1063774507030364

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