Abstract
Wax esters, which are esters of fatty alcohols and fatty acids (FAs), are one of the main classes of reserve lipids in all coral species. The chemical structures and the content of wax ester molecular species were determined for the first time in nine coral species from three taxonomic groups: symbiotic reef-building corals, (Hexacorallia subclasses), symbiotic soft coral alcyonarians, and asymbiotic soft coral gorgonians (Octocorallia subclasses) collected in the South China Sea (Vietnam). Our comparison of these groups showed that the absence of symbiotic microalgae (zooxanthellae) and the exoskeleton affects the profile of molecular species of wax esters considerably. The main components of wax esters of all corals were cetyl palmitate (16:0-16:0) and other saturated wax esters containing 30, 34, and 36 carbon atoms. The content of unsaturated molecular species 6:0–16:1, 16:0–18:1, and 16:0–20:1 in wax esters of symbiotic soft corals (alcyonarians) was greater than that in wax esters of reef-building corals. In contrast to symbiotic coral species, wax esters of asymbiotic soft corals, namely azooxanthellate gorgonians, contained a considerable amount of long-chain molecular species (C37-C41) with an odd number of carbon atoms. The presence of such molecular species indicates that asymbiotic gorgonians may use bacterial FAs in biosynthesis of their own wax esters. This observation confirms our hypothesis that bacterial community is important for maintaining the energy balance of azooxanthellate corals.
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Imbs, A.B., Fatty acids and other lipids of corals: composition, distribution and biosynthesis, Russ. J. Mar. Biol., 2013, vol. 39, no. 3, pp. 153–168.
Al-Lihaibi, S.S., Al-Sofyani, A., Niaz, G.R., et al., Long-chain wax esters and diphenylamine in fire coral Millepora dichotoma and Millepora platyphylla from Saudi Red Sea Coast, Sci. Mar., 2002, vol. 66, no. 2, pp. 95–101.
Baptista, M., Lopes, V.M., Pimentel, M.S., et al., Temporal fatty acid dynamics of the octocoral Veretillum cynomorium, Comp. Biochem. Physiol., Part B: Biochem. Mol. Biol., 2012, vol. 161, no. 2, pp. 178–187.
Beleneva, I.A., Dautova, T.I., and Zhukova, N.V., Characterization of communities of heterotrophic bacteria associated with healthy and diseased corals in Nha Trang Bay (Vietnam), Microbiology, 2005, vol. 74, no. 5, pp. 579–587.
Benson, A.A. and Muscatine, L., Wax in coral mucus: energy transfer from corals to reef fishes, Limnol. Oceanogr., 1974, vol. 19, pp. 810–814.
Brondz, I., Development of fatty acid analysis by highperformance liquid chromatography, gas chromatography, and related techniques, Anal. Chim. Acta, 2002, vol. 465, no. 1–2, pp. 1–37.
Chen, H.-K., Wang, L.-H., Chen, W.-N.U., et al., Coral lipid bodies as the relay center interconnecting diel-dependent lipidomic changes in different cellular compartments, Sci. Rep., 2017, vol. 7, Art. no. 3244.
Chen, H.-K., Song, S.-N., Wang, L.-H., et al., Compartmental comparison of major lipid species in a coral-Symbiodinium endosymbiosis: evidence that the coral host regulates lipogenesis of its cytosolic lipid bodies, PLoS One, 2015, vol. 10, p. 7.
Crossland, C.J., Barnes, D.J., and Borowitzka, M.A., Diurnal lipid and mucus production in the staghorn coral Acropora acuminata, Mar. Biol., 1980, vol. 60, pp. 81–90.
Dalsgaard, J., John, M.S., Kattner, G., et al., Fatty acid trophic markers in the pelagic marine environment, Adv. Mar. Biol., 2003, vol. 46, pp. 225–340.
Dodds, L.A., Black, K.D., Orr, H., and Roberts, J.M., Lipid biomarkers reveal geographical differences in food supply to the cold-water coral Lophelia pertusa (Scleractinia), Mar. Ecol.: Progr. Ser., 2009, vol. 397, pp. 113–124.
Fleury, B.G., Coll, J.C., Sammarco, P.W., et al., Complementary (secondary) metabolites in an octocoral competing with a scleractinian coral: Effects of varying nutrient regimes, J. Exp. Mar. Biol. Ecol., 2004, vol. 303, pp. 115–131.
Folch, J.F., Lees, M., and Sloane Stanley, G.H., A simple method for the isolation and purification of total lipids from animal tissue, J. Biol. Chem., 1957, vol. 226, pp. 497–509.
Grant, A.J., Remond, M., and Hinde, R., Low molecular-weight factor from Plesiastrea versipora (Scleractinia) that modifies release and glycerol metabolism of isolated symbiotic algae, Mar. Biol., 1998, vol. 130, pp. 553–557.
Grottoli, A.G., Rodrigues, L.J., and Juarez, C., Lipids and stable carbon isotopes in two species of Hawaiian corals, Porites compressa and Montipora verrucosa, following a bleaching event, Mar. Biol., 2004, vol. 145, pp. 621–631.
Hamoutene, D., Puestow, T., Miller-Banoub, J., and Wareham, V., Main lipid classes in some species of deep-sea corals in the Newfoundland and Labrador region (Northwest Atlantic Ocean), Coral Reefs, 2008, vol. 27, no. 1, pp. 237–246.
Harland, A.D., Davies, P.S., and Fixter, L.M., Lipid content of some Caribbean corals in relation to depth and light, Mar. Biol., 1992, vol. 113, pp. 357–361.
Harland, A.D., Fixter, L.M., Davies, P.S., and Anderson, R.A., Distribution of lipids between the zooxanthellae and animal compartment in the symbiotic sea anemone Anemonia viridis: wax esters, triglycerides and fatty acids, Mar. Biol., 1991, vol. 110, pp. 13–19.
Harland, A.D., Navarro, J.C., Davies, P.S., and Fixter, L.M., Lipids of some Caribbean and Red Sea corals: Total lipid, wax esters, triglycerides and fatty acids, Mar. Biol., 1993, vol. 117, no. 1, pp. 113–117.
Hill’manning, D.N. and Blanquet, R.S., Lipid biosynthesis in warm- and cold-acclimatized sea anemones, Metridium senile (L.), J. Exp. Mar. Biol. Ecol., 1980, vol. 48, no. 2, pp. 113–121.
Imbs, A.B., Demina, O.A., and Demidkova, D.A., Lipid class and fatty acid composition of the boreal soft coral Gersemia rubiformis, Lipids, 2006, vol. 41, no. 7, pp. 721–725.
Imbs, A.B., Latyshev, N.A., Zhukova, N.V., et al. Comparison of fatty acid compositions of azooxanthellate Dendronephthya and zooxanthellate soft coral species, Comp. Biochem. Physiol. B, 2007, vol. 148, pp. 314–321.
Joseph, J.D., Lipid composition of marine and estuarine invertebrates: Porifera and Cnidaria, Prog. Lipid Res., 1979, vol. 18, pp. 1–30.
Kaneda, T., Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance, Microbiol. Rev., 1991, vol. 55, no. 2, pp. 288–302.
Liu, Zhu-Jin, Li, Jian-Xiong, and Wu, Hou-Ming, A study on chemical constituents of South China Sea soft coral Sinularia microclavate, Chin. J. Org. Chem., 1990, vol. 10, no. 3, pp. 277–281.
Luo, Y.J., Wang, L.H., Chen, W.N.U., et al., Ratiometric imaging of gastrodermal lipid bodies in coraldinoflagellate endosymbiosis, Coral Reefs, 2009, vol. 28, no. 1, pp. 289–301.
Meyers, P.A., Polyunsaturated fatty acids in coral: indicators of nutritional sources, Mar. Biol. Lett., 1979, vol. 1, pp. 69–75.
Muscatine, L., Glycerol excretion by symbiotic algae from corals and Tridacna and its control by the host, Science, 1967, vol. 156, no. 3774, pp. 516–519.
Oku, H., Yamashiro, H., Onaga, K., et al., Seasonal changes in the content and composition of lipids in the coral Goniastrea aspera, Coral Reefs, 2003, vol. 22, pp. 83–85.
Pasby, B.F., A characterization of the lipids of the organisms which make up the main bulk of coral reef with particular emphasis on the hydrocarbons, Ph. D. Thesis, College Station: Texas A&M Univ., 1965, p. 149.
Patton, J.S., Abraham, S., and Benson, A.A., Lipogenesis in the intact coral Pocillopora capitata and its isolated zooxanthellae: evidence for a light-driven carbon cycle between symbiont and host, Mar. Biol., 1977, vol. 44, no. 3, pp. 235–247.
Patton, J.S. and Burris, J.E., Lipid synthesis and extrusion by freshly isolated zooxanthellae (symbiotic algae), Mar. Biol., 1983, vol. 75, pp. 131–136.
Rohwer, F., Breitbart, M., Jara, J., et al., Diversity of bacteria associated with the Caribbean coral Montastraea franksi, Coral Reefs, 2001, vol. 20, no. 1, pp. 85–91.
Spalding, M.D. and Grenfell, A.M., New estimates of global and regional coral reef areas, Coral Reefs, 1997, vol. 16, pp. 225–230.
Urbanova, K., Vrkoslav, V., Valterova, I., et al., Structural characterization of wax esters by electron ionization mass spectrometry, J. Lipid Res., 2011, vol. 53, no. 1, pp. 204–213.
Yamashiro, H., Oku, H., Higa, H., Chinen, I., and Sakai, K., Composition of lipids, fatty acids and sterols in Okinawan corals, Comp. Biochem. Physiol., Part B: Biochem. Mol. Biol., 1999, vol. 122, no. 4, pp. 397–407.
Yamashiro, H., Oku, H., Onaga, K., et al., Coral tumors store reduced level of lipids, J. Exp. Mar. Biol. Ecol., 2001, vol. 265, pp. 171–179.
Yamashiro, H., Oku, H., and Onaga, K., Effect of bleaching on lipid content and composition of Okinawan corals, Fish. Sci., 2005, vol. 71, no. 2, pp. 448–453.
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Original Russian Text © T.V. Bosh, P.Q. Long, 2017, published in Biologiya Morya.
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Bosh, T.V., Long, P.Q. A Comparison of the Composition of Wax Ester Molecular Species of Different Coral Groups (Subclasses Hexacorallia and Octocorallia). Russ J Mar Biol 43, 471–478 (2017). https://doi.org/10.1134/S1063074017060049
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DOI: https://doi.org/10.1134/S1063074017060049