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Somatic embryogenesis in Pinus pumila and productivity of embryogenic lines during long-term cultivation in vitro

  • Experimental Embryology
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Abstract

Zygotic embryos and megagametophytes of Pinus pumila for cultivation in vitro were transferred in 1/2 LV medium supplemented with growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (6-BAP) to induce somatic embryogenesis. Four stably proliferating cell lines from two genotypes were derived. The cell lines differed in the number of globular somatic embryos and the weight of embryogenic calli. Cells of these lines were multiplied as a result of somatic polyembryogenesis via cleavage. In the nutrient medium for maturation, mature somatic embryos were obtained. However, somatic embryos of not all embryogenic cell lines reached maturation. In this study, plantlets were obtained in an in vitro culture for the first time.

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Authors and Affiliations

  1. Sukachev Institute of Forestry, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, bld. 28, Krasnoyarsk, 660036, Russia

    I. N. Tret’yakova & D. N. Shuvaev

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  1. I. N. Tret’yakova
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  2. D. N. Shuvaev
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Correspondence to I. N. Tret’yakova.

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Original Russian Text © I.N. Tret’yakova, D.N. Shuvaev, 2015, published in Ontogenez, 2015, Vol. 46, No. 5, pp. 327–337.

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Tret’yakova, I.N., Shuvaev, D.N. Somatic embryogenesis in Pinus pumila and productivity of embryogenic lines during long-term cultivation in vitro. Russ J Dev Biol 46, 276–285 (2015). https://doi.org/10.1134/S1062360415050070

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  • DOI: https://doi.org/10.1134/S1062360415050070

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