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Bioaccumulation of silver and gold nanoparticles in organs and tissues of rats studied by neutron activation analysis

  • Animal and Human Physiology
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Abstract

Bioaccumulation of silver (Ag) and gold (Au) nanoparticles (NPs) with mean sizes of 35 nm and 6 nm, respectively, has been studied after their intragastric administration to rats at a dose of 100 μg/kg of body weight for 28 or 14 days. The organs and tissues (liver, kidney, spleen, heart, gonads, brain, and blood) were subjected to thermal neutron activation, and, then, the activity of the 110mAg and 198Au isotopes generated was measured. The NPs of both metals were detected in all biological samples studied, the highest specific weight and content of Ag NP being found in the liver, and those of Au being found in kidneys of animals. The content of Ag NPs detected in the brain was 66.4 ± 5.6 ng (36 ng/g tissue), no more than 7% of these NPs being localized in the lumen of brain blood vessels. The content of Ag and Au NPs found in organs and tissues of rats could be regarded as nonhazardous (nontoxic) in accordance with the known literature data.

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Reference

  • Abdelhalim, M.A.K. and Jarrar, B.M., Histological alterations in the liver of rats induced by different gold nanoparticle sizes, doses and exposure duration, J. Nanobiotechnol., 2012, vol. 10, no. 1, p. 5.

    Article  CAS  Google Scholar 

  • Arora, S., Jain, J., Rajwade, J.M., and Paknikar, K.M., Interactions of silver nanoparticles with primary mouse fibroblasts and liver cells, Toxicol. Appl. Pharmacol., 2009, vol. 236, no. 3, pp. 310–318.

    Article  PubMed  CAS  Google Scholar 

  • Buzulukov, Yu.P., Gmoshinskii, I.V., Raspopov, R.V., et al., Study of absorption and biodistribution of nanoparticles of some inorganic substances introduced into the gastrointestinal tract of rats using radiotracers, Med. Radiol. Radiats. Bezopasn., 2012, vol. 57, no. 3, pp. 5–12.

    Google Scholar 

  • Chaudhry, Q., Scotter, M., Blackburn, J., et al., Applications and implications of nanotechnologies for the food sector, Food Add. Contam., 2008, vol. 25, no. 3, pp. 241–258.

    Article  CAS  Google Scholar 

  • Day, E.S., Bickford, L.R., Slater, J.H., et al., Early diagnosis of oral cancer based on the surface plasmon resonance of gold nanoparticles, Int. J. Nanomed., 2010, vol. 5, pp. 445–454.

    Article  CAS  Google Scholar 

  • Gmoshinskii, I.V., Smirnova, V.V., and Khotimchenko, S.A., Current status of the problem of nanomaterial safety assessment, Ros. Nanotekhnol., 2010, vol. 5, nos. 9–10, pp. 6–10.

    Google Scholar 

  • Haase, A., Rott, S., Mantion, A., et al., Effects of silver nanoparticles on primary mixed neural cell cultures: uptake, oxidative stress and acute calcium responses, Toxicol. Sci., 2012, vol. 126, no. 2, pp. 457–468.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Hussain, S.M., Hess, K.L., Gearhart, J.M., et al., In vitro toxicity of nanoparticles in BRL 3A rat liver cells, Toxicol. in vitro, 2005, vol. 9, no. 7, pp. 975–983.

    Article  CAS  Google Scholar 

  • ISO 11929: 2010. Determination of the characteristic limits (decision threshold, detection limit and limits of the confidence interval) for measurements of ionizing radiation. Fundamentals and application, Int. Organization Standard. 2010. http://www.iso.org/iso/catalogue_detail.htm?csnumber=43810

    Google Scholar 

  • Johnston, H.J., Hutchison, G., Christensen, F.M., et al., A review of the in vivo and in vitro toxicity of silver and gold particulates: particle attributes and biological mechanisms responsible for the observed toxicity, Crit. Rev. Toxicol., 2010, vol. 40, no. 4, pp. 328–346.

    Article  PubMed  CAS  Google Scholar 

  • Khotimchenko, S.A., Gmoshinskii, I.V., and Tutel’yan, V.A., The problem of ensuring the safety of nanoscale objects for human health, Gig. Sanit., 2009, no. 5, pp. 7–12.

    Google Scholar 

  • Kim, Y.S., Kim, J.S., Cho, H.S., et al., Twenty-eight-day oral toxicity, genotoxicity, and gender-related tissue distribution of silver nanoparticles in Sprague-Dawley rats, Inhal. Toxicol., 2008, vol. 20, no. 6, pp. 575–583.

    Article  PubMed  CAS  Google Scholar 

  • Kuznetsov, R.A., Aktivatsionnyi analiz (Activation Analysis), Moscow: Atomizdat, 1974.

    Google Scholar 

  • Lee, M.S., Nam, S.I. Min, E.S., et al., Colloid solution of metal nanoparticles, metal-polymer nanocomposites and methods for preparation thereof, US Patent No. 7348365, 2008.

    Google Scholar 

  • Levard, C., Hotze, E.M., Lowry, G.V., and Brown, G.E., Environmental transformations of silver nanoparticles: impact on stability and toxicity, Environ. Sci. Technol., 2012, vol. 46, no. 13, pp. 6900–6914.

    Article  PubMed  CAS  Google Scholar 

  • Liu, Z., Ren, G., Zhang, T., and Yang, Z., Action potential changes associated with the inhibitory effects on voltagegated sodium current of hippocampal ca1 neurons by silver nanoparticles, Toxicology, 2009, vol. 264, no. 3, pp. 179–184.

    Article  PubMed  CAS  Google Scholar 

  • Loeschner, K., Hadrup, N., Qvortrup, K., et al., Distribution of silver in rats following 28 days of repeated oral exposure to silver nanoparticles or silver acetate, Part. Fibre Toxicol., 2011, vol. 8, pp. 1–18.

    Article  CAS  Google Scholar 

  • Nevzorova, V.V., Gmoshinskii, I.V., and Khotimchenko, S.A., Problems of assessing the safety of nanomaterials used in food packaging, Vopr. Pitaniya, 2009, vol. 78, no. 4, pp. 54–60.

    CAS  Google Scholar 

  • Oberdörster, G., Maynard, A., Donaldson, K., et al., Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy, Part. Fibre Toxicol., 2005, vol. 2, no. 1, pp. 8–43.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Onishchenko, G.G., Archakov, A.I., Bessonov, V.V., et al., Methodical approaches to assessing the safety of nanomaterials, Gig. Sanit., 2007, no. 6, pp. 3–10.

    Google Scholar 

  • Onishchenko, G.G. and Tutel’yan, V.A., On the concept of toxicological studies, risk assessment methodology, and methods for the identification and quantification of nanomaterials, Vopr. Pitaniya, 2007, vol. 76, no. 6, pp. 4–8.

    CAS  Google Scholar 

  • Powers, C.M., Badireddy, A.R., Ryde, I.T., et al., Silver nanoparticles compromise neurodevelopment in pc12 cells: critical contributions of silver ion, particle size, coating, and composition, Environ. Health Perspect., 2011, vol. 119, no. 1, pp. 37–44.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Raspopov, R.V., Gmoshinskii, I.V., Popov, K.I., et al., Methods of control of nanoparticles in food items and biological objects. 1. Application of microscopic and chromatographic methods of research, Vopr. Pitaniya, 2012, vol. 81, no. 2, pp. 10–17.

    Google Scholar 

  • Reese, T.S. and Karnovsky, M.J., Fine structural localization of a blood-brain barrier to exogenous peroxidase, J. Cell Biol., 1967, vol. 34, pp. 207–217.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Reeves, P.G., Nielsen, F.H., and Fahey, G.C., Jr., AIN-93 purified diets for laboratory rodents: final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet, J. Nutr., 1993, vol. 123, no. 11, pp. 1939–1951.

    PubMed  CAS  Google Scholar 

  • Safenkova, I., Zherdev, A., and Dzantiev, B., Factors influencing the detection limit of the lateral-flow sandwich immunoassay: a case study with potato virus X, Analyt. Bioanalyt. Chem., 2012, vol. 403, no. 6, pp. 1595–1605.

    Article  CAS  Google Scholar 

  • Shin, S.H., Ye, M.K., Kim, H.S., and Kang, H.S., The effects of nano-silver on the proliferation and cytokine expression by peripheral blood mononuclear cells, Int. Immunopharmacol., 2007, vol. 7, no. 13, pp. 1813–1818.

    Article  PubMed  CAS  Google Scholar 

  • Sonavane, G., Tomoda, K., Sano, A., et al., In vitro permeation of gold nanoparticles through rat skin and rat intestine: effect of particle size, Colloids Surf., 2008, vol. 65, no. 1, pp. 1–10.

    Article  CAS  Google Scholar 

  • Takakura, Y., Audus, K.L., and Borchardt, R.T., Bloodbrain barrier: transport studies in isolated brain capillaries and in cultured brain endothelial cells, Adv. Pharmacol., 1991, vol. 22, pp. 137–165.

    Article  PubMed  CAS  Google Scholar 

  • Tiede, K., Boxall, A.B., Tear, S.P., et al., Detection and characterization of engineered nanoparticles in food and the environment, Food Add. Contam., 2008, vol. 25, no. 7, pp. 795–821.

    Article  CAS  Google Scholar 

  • Vernikov, V.M., Arianova, E.A., Gmoshinskii, I.V., et al., Nanotechnologies in food production: prospects and challenges, Vopr. Pitaniya, 2009, vol. 78, no. 2, pp. 4–17.

    CAS  Google Scholar 

  • Walczak, A.P., Fokkink, R., Peters, R., et al., Behavior of silver nanoparticles and silver ions in an in vitro human gastrointestinal digestion model, Nanotoxicology, 2013. http://www.ncbi.nlm.nih.gov/pubmed/?term=Behavior+of+siver+nanoparticles+and+silver+ions+in+an+in+vitro+human+gastrointestinal+digestion+model

    Google Scholar 

  • Wiwanitkit, V., Sereemaspun, A., and Rojanathanes, R., Effect of gold nanoparticles on spermatozoa: the first world report, Fertil. Steril., 2009, vol. 91, no. 1, pp. 7–8.

    Article  Google Scholar 

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Authors and Affiliations

  1. National Research Center Kurchatov Institute, pl. Kurchatova 1, Moscow, 123182, Russia

    Yu. P. Buzulukov & V. F. Demin

  2. Institute of Nutrition, Russian Academy of Medical Sciences, Ust’inskii proezd 2/14, Moscow, 109240, Russia

    E. A. Arianova, I. V. Gmoshinski & V. A. Tutelyan

  3. Bakh Institute of Biochemistry, Russian Academy of Science, Leninskii pr. 33/2, Moscow, 119071, Russia

    I. V. Safenkova

Authors
  1. Yu. P. Buzulukov
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  2. E. A. Arianova
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  3. V. F. Demin
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  4. I. V. Safenkova
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  5. I. V. Gmoshinski
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  6. V. A. Tutelyan
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Correspondence to I. V. Gmoshinski.

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Original Russian Text © Yu.P. Buzulukov, E.A. Arianova, V.F. Demin, I.V. Safenkova, I.V. Gmoshinski, V.A. Tutelyan, 2014, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2014, No. 3, pp. 286–295.

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Buzulukov, Y.P., Arianova, E.A., Demin, V.F. et al. Bioaccumulation of silver and gold nanoparticles in organs and tissues of rats studied by neutron activation analysis. Biol Bull Russ Acad Sci 41, 255–263 (2014). https://doi.org/10.1134/S1062359014030042

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  • DOI: https://doi.org/10.1134/S1062359014030042

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