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Comparison of anticoagulant effects of regulatory proline-containing oligopeptides. Specificity of glyprolines, semax, and selank and potential for their practical application

  • Animal and Human Physiology
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Abstract

Experimental and theoretical demonstrations of different effect of certain regulatory peptides (RPs) on blood coagulation are available. The problem of the role of RPs in hemostatic processes becomes particularly significant since, first, the peptides are widespread in nature both in animal and plant tissues, second, there is a relationship between the peptide structure and function and, third, both natural and synthetic peptides are used in practical medicine to correct functions of some factors of the hemostatic system. Many peptide inhibitors of the primary and plasma hemostasis potentiating anticoagulant effects in the body have been described.

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References

  • Abumiya, T., Fitridge, R., Mazur, C., Copeland, B.R., Koziol, J.A., Tschopp, J.F., Pierschbacher, M.D., del Zoppo, G.J., and Hurn, P.D., Integrin α IIb β3 Inhibitor Preserves Microvascular Patency in Experimental Acute Focal Cerebral Ischemia, Stroke, 2000, vol. 31, pp. 1402–1410.

    CAS  PubMed  Google Scholar 

  • Ahmed, A.K., Warnock, M., Nieman, M., Srikanth, S., Mahdi, F., Krishnan, R., Tulinsky, A., and Schmaier, A.H., Mechanisms of Arg-Pro-Gly-Phe Inhibition of Thrombin, J. Physiol Heart Circ Physiol, 2003, vol. 285, pp. 183–193.

    Google Scholar 

  • Ashmarin, I.P., Pastorova, V.E., Lyapina, L.A., Kudryashov, B.A., Kalikhevich, V.A., Kamenskii, A.A., and Azieva, L.D., The effect of Tuftsin on Fibrin Polymerization in Vitro and on the Hemostatic System When Administered Intravenously to Healthy Animals, Izv. Akad. Nauk SSSR. Ser. Biol., 1991, no. 2, pp. 302–308.

  • Ashmarin, I.P., Lyapina, L.A., and Pastorova, V.E., Modulation of Hemostatic Reactions in vitro and in vivo by Representatives of Control Peptide Families, Vestn. Ross. Akad. Med. Nauk, 1996, no. 6, pp. 50–58.

  • Ashmarin, I.P., Levitskaya, N.G., Kamenskii, A.A., and Myasoedov, N.F., Semax—A New Drug for Correcting Cerebral Circulation, Hypoxic States, and Increasing Mental Activity, Farmateka, 1997, no. 4, pp. 32–33.

  • Ashmarin, I.P., Karazeeva, E.P., Lyapina, L.A., and Samonina, G.E., The Simplest Proline-Containing Peptides PG, GP, PGP and GPGG: Regulatory Activity and Possible Sources of Biosynthesis, Biokhimiya, 1998a, vol. 63, no. 2, pp. 134–141.

    Google Scholar 

  • Ashmarin, I.P., Pastorova, V.E., and Lyapina, L.A., Effect of Simple Proline-Containing Peptides on the Functional Activity of the Anticoagulant System and Primary Homeostasis, Byull. Eksp. Biol. Med., 1998b, no. 5, pp. 496–499.

  • Ashmarin, I.P., Kamenskii, A.V., Lyapina, L.A., Myasoedov, N.F., and Samonina, G.E., Glyprolines as Independent Regulators and Stabilizers of Other Peptides, Vopr. Biol. Med. Farmatsevtich. Khim., 2002, no. 1, pp. 24–27.

  • Ashmarin, I.P., Samonina, G.E., Lyapina, L.A., Kamenskiy, A.A., Levitskaya, G.N., Grivennikov, V.A., Dolotov, O.V., Andreeva, L.A., and Myasoedov, N.F., Natural and Hybrid (“Chimeric”) Stable Regulatory Glyproline Peptides, Pathophysiol, 2005, vol. 11, pp. 179–185.

    CAS  Google Scholar 

  • Bajusz, S., Barabas, E., Fauszt, J., et al., Active Site-Directed Thrombin Inhibitiors: Alpha-Hydroxydeyl-Prolyl-Arginin. New Orally Active Stable Analogs of D-Phe-Pro-Arg-H, Bioorg. Med. Chem., 1995, vol. 3, no. 8, pp. 1079–1089.

    CAS  PubMed  Google Scholar 

  • Barker, P.L., Bullens, S., and Bunting, S., Cyclic RGD Peptide Analogues as Antiplatelets Antithrombotics, J. Med. Chem., 1992, vol. 35, pp. 2040–2048.

    Article  CAS  PubMed  Google Scholar 

  • Barnes, M.J., Knight, C.G., and Farndale, R.W., The Use of Collagen-Based Model Peptides to Investigate Platelet-Reactive Sequences in Collagen, Biopolymers, 1996, vol. 40, pp. 383–397.

    Article  CAS  PubMed  Google Scholar 

  • Bienkowsk, M., Cowan, J., and McDonald, R.C., Degradation of Newly Synthesized Collagen, J. Biol. Chem., 1978, vol. 253, pp. 4356–4363.

    Google Scholar 

  • Bokarev, I.N., Laboratory Methods to Study Blood Coagulation in Clinical Practice, in Tromby, krovotochivost’ i bolezni sosudov. Prilozhenie no. 2 (Clots and Vascular Hemorrhagic Diathesis and Other Diseases), Moscow: MMA I.M. Sechenova, 2003, pp. 5–9.

    Google Scholar 

  • Byshevskii A.Sh., Chiryat’ev E.A., Umutbaeva M.K., Tazhudinova S.I., Regulator Peptide of Blood Aggregation Function at the Stage of Fibrin Self-Assembly, Gematol. Transfuziol., 1986, vol. 31, no. 6, pp. 26–31.

    CAS  PubMed  Google Scholar 

  • Cherkasova, L.A., Lyapina, L.A., and Ashmarin, I.P., Comparative Study of Semax and Simple Proline-Containing Peptides in Modulating Hemostatic Reactions, Byull. Eksp. Biol. Med., 2001, vol. 132, no. 7, pp. 20–22.

    Google Scholar 

  • Chernukh, A.M., Gomazkov, O.A., Entsik, L.A., and Krizhevskaya, Yu. V., Bradykinin, Thrombin and Prostaglandins as Modulators of Microthrombogenesis in Local Injury of the Blood Vessel Wall, Byull. Eksp. Biol. Med., 1983, no. 1, pp. 30–32.

  • Chipens, G.I. and Veretennikova, N.I., Struktura i funktsii nizkomolekulyarnykh peptidov (Structure and Functions of Low Molecular Weight Peptides), Riga: Med. in-t, 1980.

    Google Scholar 

  • Dolgov, V.V., Avdeeva, N.A., and Shchetnikovich, K.A., Metody issledovaniya gemostaza (Methods for Studying Hemostasis), Moscow: Izd-vo min-va zdravookhraneniya i medpromyshlennosti RF, 1996.

    Google Scholar 

  • Doolittle, R.F., Spraggon, G., and Everse, S.J., Crystal Structures of Fragments D and Doble-D Form Fibrinogen and Fibrin, Thromb. Haemost., 1999, vol. 82, no. 2, pp. 271–276.

    CAS  PubMed  Google Scholar 

  • Farndale, R.W., Sixma, J.J., Barens, M.J., and de Groot, P.G., The Role of Collagen in Thrombosis and Haemostasis, J. Thromb. Haemost., 2004, vol. 2, no. 4, pp. 561–573.

    Article  CAS  PubMed  Google Scholar 

  • Ganguly, K., Krasik, T., Medinilla, S., Bdeir, K., Cines, D.B., Muzykantov, V.R., and Murciano, J.C., Blood Clearance and Activity of Erythrocyte-Coupled Fibrinolytics, J. Pharmacol. Exp. Ther., 2005, vol. 312, pp. 1106–1113.

    CAS  PubMed  Google Scholar 

  • Gomazkov, O.A., Peptidy v kardiologii: Biokhimiya, fiziologiya, patologiya, informatsiya, analiz (Peptides in Cardiology: Biochemistry, Physiology, Pathology, Information, Analysis), Moscow: Materik al’fa, 2000.

    Google Scholar 

  • Gouwy, M., Struyf, S., Catusse, J., Proost, P., and Damme, J.V., Synergy between Proinflammatory Ligandes of G Protein-Coupled Receptors in Neutrophil Activation and Migration, J. Leukocyte Biol., 2004, vol. 76, pp. 185–194.

    CAS  PubMed  Google Scholar 

  • Hemker, H.C., Dieri, R.A., and Beguin, S., Laboratiry Monitoring of Low-Molecular-Weight Heparin Therapy. Part II, J. Thromb. Haemost., 2005, vol. 3, no. 3, pp. 371–376.

    Article  Google Scholar 

  • Karniguian, A., Legrand, Y.J., Lefrancier, P., and Caen, J.P., Effect of a Collagen-Derived Octapeptide on Different Steps of the Platelet/Collagen Interaction, Thromb. Res., 1983, vol. 32, pp. 593–604.

    Article  CAS  PubMed  Google Scholar 

  • Kini, R. and Evans, H.A., Novel Approach to the Design of Potent Bioactive Peptides by Incorporation of Proline Brackets: Antiplatelet Effects of Arg-Gly-Asp Peptides, FEBS Lett., 1995, vol. 375, no. 1, pp. 15–17.

    CAS  PubMed  Google Scholar 

  • Kirichuk, V.F. and Burova, M.B., Effect of Oxytocin on Fibrinolysis and the Status of the Kallikrein-Kinin System, Probl. Endokrinol., 1989, vol. 35, no. 2, pp. 81–84.

    CAS  Google Scholar 

  • Laudano, A.P. and Doolittle, R.F., Studies on Synthetic Peptides That Bind to Fibrinogen and Prevent Fibrin Polimerisation. Structural Reguirements, Number of Binding Sites, and Species Differences, Biochemistry, 1980, vol. 19, pp. 1013–1019.

    Article  CAS  PubMed  Google Scholar 

  • Levitskaya, N.G., Kleimenov, A.N., Petrosyan, M.T., Rozenfel’d, M.A., Kalikhevich, V.N., and Ardemasova, Z.A., Effect of Low-Molecular Peptides on the Fibrin-Fibrinogen Transformation, Byull. Eksp. Biol. Med., 1987, no. 7, pp. 190–192.

  • Lugovskoi, E.V., Molekulyarnye mekhanizmy obrazovaniya fibrina i fibrinoliza (Molecular Mechanisms of Fibrin Formation and Fibrinolysis), Kiev: Naukova Dumka, 2003.

    Google Scholar 

  • Lyapina, L.A., Pastorova, V.E., Kudryashov, B.A., Zazhirei, V.D., Sokolova, E.A., Livshits, V.A., and Ashmarin, I.P., The Action of Thymoptin Peptides on the Hemostatic System, Izv. Akad. Nauk SSSR. Ser. Biol., 1990, no. 3, pp. 377–382.

  • Lyapina, L.A., Pastorova, V.E., Kondashevskaya, M.V., Kudryashov, B.A., and Kalikhevich, V.N., The Antithrombotic and Thrombolytic Activity of Tuftsin, Byull. Eksp. Biol. Med., 1994, vol. 118, no. 11, pp. 1317–1319.

    Google Scholar 

  • Lyapina, L.A., Pastorova, V.E., Samonina, G.E., and Ashmarin, I.P., The Effect of Peptide PGP and PGP-Rich Preparation on Haemostatic Indexes of Rat Blood, Blood Coagulation Fibrinol., 2000, vol. 11, no. 1, pp. 1–6.

    Google Scholar 

  • Lyapina, L.A., Pastorova, V.E., Ul’yanov, A.M., Tarasov, Yu.A., and Ashmarin, I.P., Effect of Gelatin and Pro-Glu-Pro Peptide on the Anticoagulation System Status and Development of Experimental Diabetes Mellitus, Vestn. Mosk. Univ., Ser. 16, 2002, no. 1, pp. 8–10.

  • Lyapina, L.A., Pastorova, V.E., and Smolina, T.Yu., The Role of Heparin Complexes with Collagen and Its Heparin-Binding Fragments in the Activation of the Anticoagulant System, Tromboz, Gemostaz I Reologiya, 2003, vol. 2, no. 14, pp 57–61.

    Google Scholar 

  • Meschengieser, S.S., Fondevila, C.G., Frontroth, J., Santarelli, M.T., and Lazzari, M.A., Low-Intensity Oral Anticoagulation plus Low-Dose Aspirin Versus High-Intensity Oral Anticoagulation Alone: A Randomized Trial in Patients with Mechanical Prosthetic Heart Valves, J. Thorac. Cardiovasc. Surg., 1997, vol. 113, pp. 910–916.

    CAS  PubMed  Google Scholar 

  • Monnet, E. and Fauvel-Lafeve, F., A New Platelet Receptor Specific to Type III Collagen. Tyep III Collagen-Binding Protein, J. Biol. Chem., 2000, vol. 275, pp. 10907–10912.

    Article  Google Scholar 

  • Ol’binskaya, L.I. and Gofman, A.M., Lechenie i profilaktika trombozov (Treatment and Prevention of Trombosis), Moscow: VAGRIUS, 2000.

    Google Scholar 

  • Pastorova, V.E., Lyapina, L.A., Smolina, T.Yu, and Ashmarin, I.P., Anticoagulant and Fibrinolytic Effects of Proline-Containing Peptides, Izv. Ross. Akad. Nauk. Ser. Biol., 1998, no. 3, pp. 390–394.

  • Pastorova, V.E., Lyapina, L.A., Ostrovskaya, R.U., Lugovskoi, E.V., Ashmarin, I.P., and Gudasheva, T.A., Fibrin-Depolymerization Activity and the Antiplatelet Effect of Small Cyclic and Linear Proline-Containing Peptides, Izv. Akad. Nauk, Ser. Biol., 2001, no. 5, pp. 607–610.

  • Pratt, K.R., Cote, H.C.F., and Chung, D.N., The Fibrin Polymerization Poket: Three-Dimensional Structure of a 30 KDa C-Termnal γ-Chain Fragment Complexed with the Peptide Gly-Pro-Arg-Pro, Proc. Natl. Acad. Sci. USA, 1997, vol. 94, pp. 7176–7181.

    Article  CAS  PubMed  Google Scholar 

  • Ruggeri, Z., Mechanisms of Shear-Induced Platelet Adhesion and Aggregation, Thromb. Haemost, 1993, vol. 70, pp. 119–123.

    CAS  PubMed  Google Scholar 

  • Samonina, G., Lyapina, L., Kopylova, G., Pastorova, V., Jeliaznik, N., Zuykova, S., and Ashmarin, I.P., Maintenance of Gastric Mucosa Integrity Proline-Containing Peptides and Gelatin Preparation, Pathophysiol., 2000, vol. 7, pp. 69–73.

    CAS  Google Scholar 

  • Samonina, G., Ashmarin, I., and Lyapina, L., Glyproline Peptide Family Review on Bioactivity and Possible Origins, Pathophysiol., 2002, vol. 8, no. 4, pp. 229–234.

    CAS  Google Scholar 

  • Shennan, A.H., Review Antiplatelet Agents (Particularly Aspirin) Reduce the Incidence of Pre-eclampsia in Women at Risk, Evid. Based. Med., 2005, vol. 10, p. 49.

    Google Scholar 

  • Shiffman, F.D., Patofiziologiya krovi (Blood Pathophysiology), Moscow: Binom, 2000.

    Google Scholar 

  • Siljander, P.R., M., Hamaia S., Peachey A.R., Slatter D.A., Smethurst P.A., Ourrehand, W.H., Knight, C.G., and Farndale, R.W., Integrin Activation State Determines Selectivity for Novel Recognition Sites Fibrillar Collagen, J. Biol. Chem., 2004, vol. 279, no. 46, pp. 47763–47772.

    Article  CAS  PubMed  Google Scholar 

  • Staatz, W.D., Fok, K.F., Zutter, M.M., Adams, S.P., Rodriguez, B.A., and Santoro, S.A., Identification of a Tetrapeptide Recognition Sequence for the α2β1 Integrin in Collagen, J. Biol. Chem., 1991, vol. 266, pp. 7363–7367.

    CAS  PubMed  Google Scholar 

  • Xu, Y., Gurusiddappa, S., Rich, R.I., Owens, R.T., Keene, D.R., Mayne, R., Hook, A., and Hook, M., Multiple Binding Sites in Collagen Type I for the Integrins α1β1 and α2β1, J. Biol. Chem., 2000, vol. 275, pp. 38981–38989.

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Biological Faculty, Moscow State University, Vorob’evy gory, Moscow, 119992, Russia

    L. A. Lyapina, V. E. Pastorova, T. Yu. Obergan, G. E. Samonina & I. P. Ashmarin

  2. Institute of Molecular Genetics, Russian Academy of Sciences, pl. Kurchatova 2, Moscow, 123182, Russia

    N. F. Myasoedov

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  1. L. A. Lyapina
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  2. V. E. Pastorova
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  3. T. Yu. Obergan
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  4. G. E. Samonina
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  5. I. P. Ashmarin
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  6. N. F. Myasoedov
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Original Russian Text © L.A. Lyapina, V.E. Pastorova, T.Yu. Obergan, G.E. Samonina, I.P. Ashmarin, N.F. Myasoedov, 2006, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2006, No. 2, pp. 193–203.

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Lyapina, L.A., Pastorova, V.E., Obergan, T.Y. et al. Comparison of anticoagulant effects of regulatory proline-containing oligopeptides. Specificity of glyprolines, semax, and selank and potential for their practical application. Biol Bull Russ Acad Sci 33, 153–161 (2006). https://doi.org/10.1134/S1062359006020099

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  • DOI: https://doi.org/10.1134/S1062359006020099

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