Abstract
A scanning near-field optical microscope (SNOM)—based modification of the method to study the dynamics of single molecule receptor—ligand interactions exploiting the fluorescence imaging by total internal reflection fluorescence microscopy is introduced. The main advantage of this approach consists in the possibility to study the single molecule interaction dynamics with a subwavelength spatial resolution and a submillisecond time resolution. Additionally, due to the much smaller irradiation area and some other technical features, such a modification enables to enlarge the scope of the receptor—ligand pairs to be investigated and to improve the temporal resolution. We briefly discuss corresponding experimental set up with a special accent on the SNOM operation in liquid and present some preliminary results of related investigations.
Similar content being viewed by others
References
T. Funatsu, Y. Harada, M. Tokunaga, K. Saito, and T. Yanagida, Nature 374, 555 (1995).
R. D. Valle, T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida, Nature 380, 451 (1996).
H. Taguchi, T. Ueno, H. Tadakuma, M. Yoshida, and T. Funatsu, Nature Biotechnol. 19, 861 (2001).
J. Kozuka, H. Yokota, Y. Arai, Y. Ishii, and T. Yanagida, Nature Chem. Biol. 2, 83 (2006).
Y. Teramura, J. Ichinose, H. Takagi, K. Nishida, T. Yanagida, and Y. Sako, EMBO J. 25, 4215 (2006).
M. Nishikawa, H. Takagi, T. Shibata, A. H. Iwane, and T. Yanagida, Phys. Rev. Lett. 101, 128103 (2008).
Y. Ishii and T. Yanagida, HFSP J. 1, 15 (2007).
T. Yanagida, M. Iwaki, and Y. Ishii, Phil. Trans. R. Soc. B: Bio. Sci. 363, 2123 (2008).
G. I. Bell, Science 200, 618 (1978).
Chemical Relaxation in Molecular Biology, Ed. by R. Rigler and I. Pecht (Springer, Heidelberg, 1976).
U. Dammer, M. Hegner, D. Anselmetti, P. Wagner, M. Dreier, W. Huber, and H. J. Guentherodt, Biophys. J. 70, 2437 (1996).
M. Martinez-Yamout and H. M. McConnell, J. Mol. Biol. 244, 301 (1994).
F. Schwesinger, R. Ros, T. Strunz, D. Anselmetti, H.-J. Güntherodt, A. Honegger, L. Jermutus, L. Tiefenauer, and A. Plückthun, Proc. Natl. Acad. Sci. USA 97, 9972 (2000).
L. Chtcheglova, “Quasistatic and Dynamic Force Spectroscopy of Single Antigen-Aantibody Complexes and Fibrin—Fibrinogen Systems,” Thesis No. 2946 (EPFL, Lausanne, 2004), unpublished.
S. K. Sekatskii, Phil. Trans. R. Soc. A 362, 901 (2004); The whole issue of the journal is devoted to SNOM review so see also other papers therein.
L. Aigouy, Y. de Wilde, and C. Frétigny, Les nouvelles microscopies. A la découvert du nanomonde, Ch. 3 (Belin, Paris, 2006).
Nano-optics and Near-field Optical Microscopy, Ed. by A. V. Zayats and D. Richards (Artech House, London, 2008).
S. K. Sekatskii, G. Dietler, and V. S. Letokhov, Chem. Phys. Lett. 452, 220 (2008).
D. V. Serebryakov, S. K. Sekatskii, A. P. Cherkun, K. Dukenbayev, I. V. Morozov, V. S. Letokhov, and G. Dietler, J. Microsc. 229, 287 (2008).
A. P. Cherkun, D. V. Serebryakov, S. K. Sekatskii, I. V. Morozov, and V. S. Letokhov, Rev. Sci. Instrum. 77, 033703 (2006).
D. V. Serebryakov, A. P. Cherkun, B. A. Loginov, and V. S. Letokhov, Rev. Sci. Instrum. 73, 1795 (2002).
A. Naber, H.-J. Maas, K. Razavi, and U. C. Fischer, Rev. Sci. Instrum. 70, 3955 (1999).
W. H. J. Rensen, N. F. Van Hulst, and S. B. Kammer, Appl. Phys. Lett. 71, 1557 (2000).
F. H. Lei, J.-F. Angiboust, W. Qiao, G. D. Sockalingum, S. Dukic, L. Chrit, M. Troyon, and M. Manfait, J. Microsc. 216, 229 (2004).
P. Lambelet, M. Pfeffer, A. Sayah, and F. Marquis-Weible, Ultramicroscopy 71, 117 (1998).
D. A. Lapshin, V. N. Reshetov, S. K. Sekatskii, and V. S. Letokhov, JETP Lett. 67, 245 (1998).
P. Tsai and Y. Y. Lu, Appl. Phys. Lett. 73, 2724 (1998).
D. N. Davydov, K. N. Shelimov, T. L. Haslett, and M. Moskovits, Appl. Phys. Lett. 75, 1796 (1999).
C. Hoeppener, J. P. Siebrasse, R. Peters, U. Kubitscheck, and A. Naber, Biophys. J. 88, 3681 (2005).
M. Koopman, B. I. de Bakker, M. F. Garcia-Parajo, and N. F. Van Hulst, Appl. Phys. Lett. 83, 5083 (1999).
M. Dittrich and S. Sibler, J. Colloid Interface Sci. 286, 487 (2005).
N. N. Denisov, L. A. Chtcheglova, S. K. Sekatskii, and G. Dietler, Colloids Surf. B: Biointerfaces 63, 282 (2008).
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © Astro, Ltd., 2010.
The article is published in the original.
Rights and permissions
About this article
Cite this article
Mensi, M., Dukenbayev, K., Sekatskii, S.K. et al. Scanning near-field optical microscopy-based study of local dynamics of receptor-ligand interactions at the single molecule level. Laser Phys. 20, 78–84 (2010). https://doi.org/10.1134/S1054660X09170125
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1054660X09170125