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qPCR Identification of the kdr Allele F1534C in Voltage-Gated Sodium Channel Gene (vgsc) of the Major Mosquito Vectors Aedes aegypti and Aedes albopictus in Northern and Central Vietnam

  • ANIMAL GENETICS
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Abstract

Pyrethroid insecticides are currently the main tool for controlling major mosquito vectors of hemorrhagic fevers—Aedes aegypti and Ae. albopictus. The widespread use of insecticides has led to the spread of insecticide resistance mutations (or kdr mutations) in mosquito populations. A population genetic study of kdr mutations provides information on changes in the genetic structure of mosquito populations as a result of anthropogenic impact and may be useful for making epidemiological prediction for the prevalence of Dengue and Chikungunya fevers. Multiplex PCR is traditionally used to identify kdr mutations in combination with sequencing of PCR fragments. We have developed a more productive method for identifying kdr mutations based on the SNP polymorphism analysis in the vgsc gene and qPCR. We identified the kdr mutation F1534C in Aedes aegypti and Ae. albopictus from the Ha Tinh province of Vietnam. In Ae. albopictus populations from Vietnam, this mutation was identified for the first time.

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Funding

This study was carried out within the scope of government order no. 0112-2019-0002 for the topic “Study of Autonomic Genetic Element Variability in Insects and Development of New Markers of Genome Instability” (АААА-А16-116111610180-3).

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Authors and Affiliations

  1. Vavilov Institute of General Genetics Russian Academy of Sciences, 119991, Moscow, Russia

    B. V. Andrianov & I. I. Goryacheva

  2. Moscow Region State University, 141014, Moscow, Russia

    T. X. Vu

  3. National Institute of Malariology, Parasitology, and Entomology, BC10200, Hanoi, Vietnam

    D. C. Vu

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  1. T. X. Vu
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  2. B. V. Andrianov
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  3. D. C. Vu
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  4. I. I. Goryacheva
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Correspondence to B. V. Andrianov.

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This study was carried out in accordance with all applicable international, national, and/or institutional principles of animal treatment.

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The authors declare that they have no conflict of interest.

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All authors made equal contribution to the study.

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Translated by M. Bibov

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Vu, T.X., Andrianov, B.V., Vu, D.C. et al. qPCR Identification of the kdr Allele F1534C in Voltage-Gated Sodium Channel Gene (vgsc) of the Major Mosquito Vectors Aedes aegypti and Aedes albopictus in Northern and Central Vietnam. Russ J Genet 56, 460–469 (2020). https://doi.org/10.1134/S1022795420040158

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