Skip to main content
SpringerLink
Log in

Polymorphism of PIT-1 and Prolactin Genes and Their Effects on Milk Yield in Holstein Frisian Dairy Cows Bred in Vietnam

  • Animal Genetics
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

The aim of this study is to investigate the polymorphism of the Pituitary transcription factor (PIT-1) and Prolactin (PRL) genes in Holstein Friesian cows (HF) bred in Vietnam and their effects on milk yields. For these purposes, 125 HF dairy cows of milking age from Da Lat Milk Farm located in Lam Dong, Vietnam were sampled. Phenotypic data of milk yields (calculated on 305-day of milking cycle; kg/day) of each individual milking cow were collected. The PIT-1.E2, PIT-1.E6, PRL.E3 and PRL.E4 genotypes were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with restriction enzymes TaqI, HinfI, RsaI, respectively. The significant effects of PIT-1.E6, PRL.E3 and PRL.E4 polymorphisms on milk yields were not observed in HF dairy cows (P > 0.05). However, results revealed statistically significant associations between PIT-1.E2 polymorphism and milk yields of HF dairy cows; individuals carrying the AA genotype yielded more milk compared to others. Our study suggests the potential use of PIT-1.E2 SNP in marker assisted selection (MAS) for improved milk yields of HF dairy cows bred in Vietnam.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Smaragdov, M.G., Genomic selection as a possible accelerator of traditional selection, Russ. J. Genet., 2009, vol. 45, pp. 633–636.

    Article  CAS  Google Scholar 

  2. Smaragdov, M.G., Analysis of chromosomal localization of loci controlling milk production traits in cattle, Russ. J. Genet., 2008, vol. 44, pp. 722–726.

    Article  CAS  Google Scholar 

  3. Yudin, N.S. and Voevoda, M.I., Molecular genetic markers of economically important traits in dairy cattle, Russ. J. Genet., 2015, vol. 51, pp. 506–517.

    Article  CAS  Google Scholar 

  4. Cohen, L.E., Wondisford, F.E., and Radovick, S., Role of PIT-1 in the gene expression of growth hormone, prolactin and thyrotropin, Endocrinol. Metab. Clin. North Am., 1997, vol. 25, pp. 523–540.

    Article  Google Scholar 

  5. Tuggle, C.K. and Trenkle, A., Control of growth hormone synthesis, Domest. Anim. Endocrinol., 1996, vol. 13, no. 1, pp. 1–33.

    Article  CAS  PubMed  Google Scholar 

  6. Moody, D.E., Pomp, D., and Berendse, W., Restriction fragment length polymorphism in amplification products of the bovine PIT-1 gene and assignment of PIT-1 to bovine chromosome 1, Anim. Genet., 1995, vol. 26, no. 1, pp. 45–47.

    Article  CAS  PubMed  Google Scholar 

  7. Renaville, R., Gengler, N., Vrech, E., et al., PIT-1 gene polymorphism, milk yield, and conformation traits for Italian Holstein Friesian bulls, J. Dairy Sci., 1997, vol. 80, no. 12, pp. 3431–3438.

    Article  CAS  PubMed  Google Scholar 

  8. Zhang, C., Liu, B., Chen, H., et al., Associations of a HinfI PCR-RFLP of POU1F1 gene with growth traits in Qinchuan cattle, Anim. Biotechnol., 2009, vol. 20, no. 2, pp. 71–74.

    Article  CAS  PubMed  Google Scholar 

  9. Heidari, M., Azari, M.A., Hasani, S., et al., Effect of polymorphic variants of GH, Pit-1, and β-LG genes on milk production of Holstein cows, Russ. J. Genet., 2012, vol. 48, pp. 417–421.

    Article  CAS  Google Scholar 

  10. Doosti, A., Arshi, A., and Momeni, B., Molecular study of PIT-1 gene polymorphism in Holstein and Iranian native cattle, Afr. J. Agric. Res., 2011, vol. 6, pp. 4467–4470.

    Google Scholar 

  11. Aytekin, İ. and Boztepe, S., Associations of PIT-1 gene polymorphism with milk yield and composition traits in Brown Swiss cattle, J. Anim. Plant Sci., 2013, vol. 23, no. 5, pp. 1281–1289.

    CAS  Google Scholar 

  12. Pan, C., Lan, X., Chen, H., et al., A TaqI PCR-RFLP detecting a novel SNP in exon 2 of the Bovine POU1F1 gene, Biochem. Genet., 2008, vol. 46, pp. 424–432.

    Article  CAS  PubMed  Google Scholar 

  13. Selvaggi, M. and Dario, C., Analysis of two PIT-1 gene polymorphisms: single nucleotide polymorphisms (SNPs) distribution patterns in Podolica cattle breed, Afr. J. Biotechnol., 2011, vol. 10, no. 55, pp. 11360–11364.

    CAS  Google Scholar 

  14. Ankur, C., Madhu, T., Satyendra, P.S., et al., Association of PIT-1 gene polymorphism milk production traits in Sahiwal cattle, Indian J. Anim. Sci., 2015, vol. 85, no. 6, pp. 610–612.

    Google Scholar 

  15. Bole-Feysot, C.H., Goffin, V., Edery, M., et al., Prolactin and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice, Endocr. Rev., 1998, vol. 19, pp. 225–268.

    Article  CAS  PubMed  Google Scholar 

  16. Barendse, W., Vaiman, D., Kemp, S.J., et al., A medium-density genetic linkage map of the bovine genome, Mamm. Genome, 1997, vol. 8, pp. 21–28.

    Article  CAS  PubMed  Google Scholar 

  17. Wallis, M., The primary structure of bovine PRL, Fed Euro Biochem. Soc. Lett., 1974, vol. 44, pp. 205–208.

    Article  CAS  Google Scholar 

  18. Sasavage, N.L., Nilsson, J.H., Horowitz, S., and Rotman, F.M., Nucleotide sequence of bovine prolactin messenger RNA, J. Biol. Chem., 1982, vol. 24, pp. 678–681.

    Google Scholar 

  19. Brym, P., Kamiñski, S., and Wójcik, E., Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits, J. Appl. Genet., 2005, vol. 45, no. 2, pp. 179–185.

    Google Scholar 

  20. Mehmannavaz, Y., Amirinia, C., Bonyadi, M., and Torshizi, R.V., Effects of bovine Prolactin gene polymorphism within exon 4 on milk related traits and genetic trends in Iranian Holstein bulls, Afr. J. Biotechnol., 2009, vol. 8, pp. 4797–4801.

    CAS  Google Scholar 

  21. Ausubel, F.M., Brent, R., Kingston, R.E., et al., Short Protocols in Molecular Biology, New York: John Wilet, 1995, 3rd ed.

    Google Scholar 

  22. Woollard, J., Schmitz, C., Freeman, A., and Tuggle, C., Rapid communication: HinfI polymorphism at the bovine PIT-1 locus, J. Anim. Sci., 1994, vol. 72, p. 3267.

    Article  CAS  PubMed  Google Scholar 

  23. Dybus, A., Grzesiak, W., Kamieniecki, H., et al., Association of genetic variants of bovine prolactin with milk production traits of Black and White and Jersey cattle, Arch. Tierz., 2005, vol. 48, pp. 149–156.

    CAS  Google Scholar 

  24. Dybus, A., Szatkowska, E., Czerniawska-Piatkowska, I., et al., PIT-1/HinfI gene polymorphism and its associations with milk production traits in polish Black-and- White cattle, Arch. Tierz. Dummerstorf, 2004, vol. 47, pp. 557–563.

    CAS  Google Scholar 

  25. César, C., Edy, A., Reyna, R., et al., Polymorphism of the prolactin gene (PRL) and its relationship with milk production in American Swiss, Afr. J. Biotechnol., 2012, vol. 11, no. 29, pp. 7338–7343.

    Google Scholar 

  26. Ghasemi, N., Zadehrahmani, M., Rahimi, G., and Hafezian, S.H., Associations between prolactin gene polymorphism and milk production in Montebeliard Cows, Int. J. Genet. Mol. Biol., 2009, vol. 1, no. 3, pp. 48–96.

    CAS  Google Scholar 

  27. Chrenek, P., Huba, J., Vasicek, D., et al., The relation between genetic polymorphism markers and milk yield in Brown Swiss cattle imported to Slovakia, Asian Aust. J. Anim. Sci., 2003, vol. 16, no. 10, pp. 1397–1401.

    Article  CAS  Google Scholar 

  28. Mitra, A, Schlee, P., Balakrishnan, C.R., and Pirchner, F., Polymorphisms at growth-hormone and prolactin loci in Indian cattle and buffalo, J. Anim. Breed. Gene, 1995, vol. 112, pp. 71–74.

    Article  Google Scholar 

  29. Chung, E.R., Rhim, T.J., and Han, S.K., Association PCR-RFLP markers of growth hormone and prolactin genes and production traits in dairy cattle, Korean J. Ani. Sci., 1996, vol. 38, pp. 321–336.

    CAS  Google Scholar 

  30. Rorie, R.W., Howland, E.M., and Lester, T.D., Evaluation of a polymorphism in the prolactin gene as a potential genetic marker for mastitis susceptibility and milk production, Arkansas Anim. Sci. Dep. Rep., 2009, vol. 21, pp. 24–25.

    Google Scholar 

  31. Ratna, K.A., Singh, K.M., Soni, K.J., et al., Genotyping of the polymorphism within exon 3 of Prolactin gene in various dairy breeds by PCR-RFLP, Arch. Tierz., 2008, vol. 51, no. 3, pp. 298–299.

    Google Scholar 

  32. Golijow, C.D., Giovambattista, G., Rípoli, M.V., et al., Genetic variability and population structure in loci related to milk production traits in native Argentine Creole and commercial Argentine Holstein cattle, Genet. Mol. Biol., 1999, vol. 22, no. 3, pp. 395–398.

    Article  Google Scholar 

  33. Zwierzchowski, L., Krzyzewski, J., Strzalkowska, N., et al., Effects of polymorphism of growth hormone (GH), PIT-1, and leptin (LEP) genes, cow’s age, lactation stage and somatic cell count on milk yield and composition of Polish Black-and-White cows, Anim. Sci. Pap. Rep., 2002, vol. 20, no. 2, pp. 213–227.

    CAS  Google Scholar 

  34. Zakizadeh, S., Reissmann, M., Rahimi, G., et al., Polymorphism of bovine POU1F1 gene: allele frequencies and effects on milk production in three Iranian native breeds and Holstein cattle of Iran, Pakistan J. Biol. Sci., 2007, vol. 10, no. 15, pp. 2575–2578.

    Article  CAS  Google Scholar 

  35. Khatami, S.R., Lazebny, O.E., Maksimenko, V.F., and Sulimova, G.E., Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and Black-and-White cattle, Russ. J. Genet., 2005, vol. 41, pp. 167–173.

    Article  CAS  Google Scholar 

  36. Alipanah, M., Kalashnikova, L., and Rodionov, G., Association of PRL gene variants with milk production traits in Russian Red Pied cattle Iran, J. Biotechnol., 2007, vol. 5, no. 3, pp. 158–161.

    CAS  Google Scholar 

  37. Boleckova J., Matejickova J., Stipkova M., et al. The association of five polymorphisms with milk production trans in Czech Fleckvieh cattle, Czech. J. Anim. Sci., 2012, vol. 57, no. 2, pp. 45–53.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam

    N. T. D. Thuy, N. T. Thu, N. H. Cuong & L. V. Ty

  2. Vietnam National University of Agriculture, Hanoi, Viet Nam

    T. T. B. Nguyen

  3. College of Agriculture and Applied Biology, Can Tho University, CanTho, Viet Nam

    D. V. A. Khoa

Authors
  1. N. T. D. Thuy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. T. Thu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. H. Cuong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. V. Ty
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. T. B. Nguyen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. V. A. Khoa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. T. D. Thuy.

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Thuy, N.T.D., Thu, N.T., Cuong, N.H. et al. Polymorphism of PIT-1 and Prolactin Genes and Their Effects on Milk Yield in Holstein Frisian Dairy Cows Bred in Vietnam. Russ J Genet 54, 346–352 (2018). https://doi.org/10.1134/S1022795418030146

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795418030146

Keywords

Search

Navigation