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Comparative analysis of Agropyron intermedium (Host) Beauv 6Agi and 6Agi2 chromosomes in bread wheat cultivars and lines with wheat–wheatgrass substitutions

  • Plant Genetics
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Abstract

A comparative study of wheat–wheatgrass substituted cultivars and lines resistant to leaf rust developed by the Agricultural Research Institute for Southeast Regions (Multi 6R, Belyanka, Favorit, Voevoda, Lebedushka) and Samara Agricultural Research Institute (Tulaikovskaya 5, Tulaikovskaya 10, Tulaikovskaya 100, Tulaikovskaya Zolotistaya) breeding was conducted. A complex analysis using molecular cytogenetic (C-differential banding, fluorescent (FISH) and genomic (GISH) in situ hybridization), molecular (PLUG markers), and biochemical (electrophoretic analysis of gliadins) markers demonstrated that they have a substitition of wheat chromosome 6D by the chromosomes 6Agi and 6Agi2 belonging to the J(=E) Agropyron intermedium (Host) Beauv (=Thinopyrum intermedium (Host) Barkworth & D.R. Dewey) subgenome. In spite of the fact that the chromosomes 6Agi and 6Agi2 differ in the C-banding pattern and demonstrated minor differences in the blocks of gliadin components, they had the identical pattern of pSc119.2 and pAs1 probe distribution and conjugated between themselves with insignificant disturbance. Thus, it was demonstrated that 6Agi and 6Agi2 are homologous chromosomes; however, the question about allelism of their leaf rust resistance genes between themselves requires special studies. Nevertheless, using STS and SCAR markers and taking into account the type of reaction to Puccinia triticina, their non-allelism to the Lr9, Lr19, Lr24, Lr29, Lr38, and Lr47 genes was established. It was revealed that the 6Agi and 6Agi2 chromosomes have a different level of transmission in hybrid F2 populations depending on the hybrid combination gene background.

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Authors and Affiliations

  1. Agricultural Research Institute for Southeast Regions, Saratov, 410010, Russia

    S. N. Sibikeev & A. E. Druzhin

  2. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    E. D. Badaeva, A. A. Shishkina & A. Yu. Dragovich

  3. All-Russian Research Institute of Plant Protection, St. Petersburg–Pushkin, 196608, Russia

    E. I. Gultyaeva

  4. Center for Molecular Biotechnology, Russian State Agrarian University–Moscow Timiryazev Agricultural Academy, Moscow, 127550, Russia

    P. Yu. Kroupin, G. I. Karlov, Thi Mai Khuat & M. G. Divashuk

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  1. S. N. Sibikeev
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  2. E. D. Badaeva
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  3. E. I. Gultyaeva
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  4. A. E. Druzhin
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  5. A. A. Shishkina
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  6. A. Yu. Dragovich
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  10. M. G. Divashuk
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Correspondence to S. N. Sibikeev.

Additional information

Original Russian Text © S.N. Sibikeev, E.D. Badaeva, E.I. Gultyaeva, A.E. Druzhin, A.A. Shishkina, A.Yu. Dragovich, P.Yu. Kroupin, G.I. Karlov, Thi Mai Khuat, M.G. Divashuk, 2017, published in Genetika, 2017, Vol. 53, No. 3, pp. 298–309.

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Sibikeev, S.N., Badaeva, E.D., Gultyaeva, E.I. et al. Comparative analysis of Agropyron intermedium (Host) Beauv 6Agi and 6Agi2 chromosomes in bread wheat cultivars and lines with wheat–wheatgrass substitutions. Russ J Genet 53, 314–324 (2017). https://doi.org/10.1134/S1022795417030115

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  • DOI: https://doi.org/10.1134/S1022795417030115

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