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DNA barcoding of fishes in Irtysh River China

  • Animal Genetics
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Abstract

DNA barcoding was a molecular diagnostic method that provided rapid and accurate species identification. The 650 bp-length cytochrome c oxidase subunit I (COI) gene of 33 species in Irtysh River China was sequenced and analyzed in this study. The average intra-species, -genus, -family, and -order of Kimura two parameter (K2P) distances were 0.003, 0.060, 0.163 and 0.240, respectively. The genetic distance between genus Barbatula and Cobitis was the largest whereas that between genus Hypophthalmichthys and Aristichthys was the smallest. The neighbour-joining tree constructed by all 44 haplotypes was divided into two major clusters: Cypriniformes fishes and other fishes. A cryptic species of Barbatula barbatula was detected according to 2% genetic threshold.

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Authors and Affiliations

  1. Xinjiang Fisheries Research Institute, Urumqi, Xinjiang, 830000, China

    Tianyan Yang, Wei Meng, Renming Zhang, Lingang Cai & Sa Hai

  2. Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, Shandong, 266003, China

    Tianyan Yang & Tianxiang Gao

  3. Fisheries College of Huazhong Agricultural University, Wuhan, Hubei, 430070, China

    Qiong Zhou

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  1. Tianyan Yang
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  2. Wei Meng
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Correspondence to Wei Meng.

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Yang, T., Meng, W., Zhang, R. et al. DNA barcoding of fishes in Irtysh River China. Russ J Genet 52, 969–976 (2016). https://doi.org/10.1134/S1022795416090167

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  • DOI: https://doi.org/10.1134/S1022795416090167

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