Abstract
The expression of genes Sox2, Klf4, Myc, Sall4, Gata6, Foxa2, Hnf4a, Cdx2, Esrrb, Hand1 in cell lines, embryos and organs of adult voles Microtus rossiaemeridionalis was studied. High resemblance of the expression patterns of these genes in the organs of adult voles, mice and humans was demonstrated. It was established that genes Gata6, Foxa2 and Hnf4a were specifically expressed in vole extraembryonic endoderm cells, while Cdx2 and Hand1 genes, in trophoblast stem cells. This shows that these genes can be used as markers of corresponding vole cell lines. Indirect confirmation pointing to the fact that Oct4 gene is a marker gene for epiblast cells both in the vole and mouse was obtained.
References
Ralston, A. and Rossant, J., Genetic Regulation of Stem Cell Origins in the Mouse Embryo, Clin. Genet., 2005, vol. 68, no. 2, pp. 106–112.
Medvedev, S.P., Shevchenko, A.I., Elisaphenko, E.A., et al., Structure and Expression Pattern of Oct4 Gene Are Conserved in Vole Microtus rossiaemeridionalis, BMC Genomics, 2008, vol. 9, pp. 162–112.
Medvedev, S.P., Elisafenko, E.A., Shevchenko, A.I., etal., Nanog Gene: Molecular Genetic Organization and Expression in the Vole Microtus rossiaemeridionalis, Dokl. Akad. Nauk, 2009, vol. 425, no. 5, pp. 688–691.
Grigor’eva, E.V., Shevchenko, A.I., Mazurok, N.A., FGF4 Independent Derivation of Trophoblast Stem Cells from the Common Vole, PLoS One, 2009, vol. 4, no. 9, pp. e 7161.
Shevchenko, A.I., Demina, V.V., Mazurok, N.A.,et al., Extraembryonic Endoderm Stem Cell Lines from Common Voles of the Genus Microtus, Russ. J. Genet., 2008, vol. 44, no. 11, p. 1290–1295.
Amati, B., Alevizopoulos, K., and Vlach, J., Myc and the Cell Cycle, Front. Biosci., 1998, vol. 3, pp. 250–268.
Rowland, B.D., Bernards, R., and Peeper, D.S., The KLF4 Tumour Suppressor Is a Transcriptional Repressor of p53 That Acts as a Context-Dependent Oncogene, Nat. Cell. Biol., 2005, vol. 7, no. 11, pp. 1074–1082.
Knoepfler, P.S., Zhang, X.Y., Cheng, P.F., et al., Myc Influences Global Chromatin Structure, EMBO J., 2006, vol. 25, no. 12, pp. 2723–2734.
Fuchs, E. and Segre, J.A., Stem Cells: A New Lease on Life, Cell, 2000, vol. 100, no. 1, pp. 143–155.
Kaestner, K.H., Hiemisch, H., Luckow, B., and Schutz, G., The HNF-3 Gene Family of Transcription Factors in Mice: Gene Structure, cDNA Sequence, and mRNA Distribution, Genomics, 1994, vol. 20, no. 3, pp. 377–385.
Morrisey, E.E., Ip, H.S., Lu, M.M., and Parmacek, M.S., GATA-6: A Zinc Finger Transcription Factor That Is Expressed in Multiple Cell Lineages Derived from Lateral Mesoderm, Dev. Biol., 1996, vol. 177, no. 1, pp. 309–322.
Suzuki, E., Evans, T., Lowry, J., et al., The Humans GATA-6 Gene: Structure, Chromosomal Location, and Regulation of Expression by Tissue-Specific and Mitogen-Responsive Signals, Genomics, 1996, vol. 38, no. 3, pp. 283–290.
Kunath, T., Arnaud, D., Uy, G.D., et al., Imprinted X-Inactivation in Extra-Embryonic Endoderm Cell Lines from Mouse Blastocysts, Development, 2005, vol. 132, no. 7, pp. 1649–1661.
Tanaka, S., Kunath, T, Hadjantonakis, A.K., et al., Promotion of Trophoblast Stem Cell Proliferation by FGF4, Science, 1998, vol. 282, no. 5396, pp. 2072–2075.
Luo, J., Sladek, R., Bader, J.A., et al., Placental Abnormalities in Mouse Embryos Lacking the Orphan Nuclear Receptor ERR-Beta, Nature, 1997, vol. 388, no. 6644, pp. 778–782.
Van den Berg, D.L., Zhang, W., Yates, A., et al., Estrogen-Related Receptor Beta Interacts with Oct4 to Positively Regulate Nanog Gene Expression, Mol. Cell. Biol., 2008, vol. 28, no. 19, pp. 5986–5995.
Elling, U., Klasen, C., Eisenberger, T., et al., Murine Inner Cell Mass-Derived Lineages Depend on Sall4 Function, Proc. Natl. Acad. Sci. USA, 2006, vol. 103, no. 44, pp. 16319–16324.
Yuri, S., Fujimura, S., Nimura, K., et al., Sall4 Is Essential for Stabilization, but not for Pluripotency, of Embryonic Stem Cells by Repressing Aberrant Tro-phectoderm Gene Expression, Stem Cells, 2009, vol. 27, no. 4, pp. 796–805.
Shields, J.M., Christy, R.J., and Yang, V.W., Identification and Characterization of a Gene Encoding a Gut-Enriched Kruppel-Like Factor Expressed during Growth Arrest, J. Biol. Chem., 1996, vol. 271, no. 33, pp. 20009–20017.
Cauffman, G., Van de Velde, H., Liebaers, I., and Van Steirteghem, A, Oct4 mRNA and Protein Expression during Humans Preimplantation Development, Mol. Hum. Reprod., 2005, vol. 11, no. 3, pp. 173–181.
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Original Russian Text © M.A. Sorokin, S.P. Medvedev, A.I. Shevchenko, N.M. Slynko, S.M. Zakian, 2010, published in Genetika, 2010, Vol. 46, No. 2, pp. 282–286.
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Sorokin, M.A., Medvedev, S.P., Shevchenko, A.I. et al. Expression of early developmental genes in vole Microtus rossiaemeridionalis . Russ J Genet 46, 249–252 (2010). https://doi.org/10.1134/S102279541002016X
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DOI: https://doi.org/10.1134/S102279541002016X