Skip to main content
SpringerLink
Log in

Molecular genetic marker-based approaches to the verification of lilac Syringa vulgaris L. in vitro germplasm collections

  • Plant Genetics
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

RAPD analysis was used to verify the varieties in an in vitro germplasm collection of lilac Syringa vulgaris L. RAPD patterns were obtained with 16 decanucleotide primers for 46 accessions (microclones and corresponding reference varieties). The RAPD patterns of a microclone and the corresponding reference variety often differed in composition; consequently, it was infeasible to verify the accessions by direct comparison of the RAPD patterns. Hence, evaluation of the relative genetic distances between accessions (microclones) and known varieties was proposed as a method to verify lilac in vitro germplasm collections.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Luneva, Z.S., Mikhailov, E.A., and Sudakova, E.A., Siren’ (Lilac), Moscow: Agropromizdat, 1989.

    Google Scholar 

  2. Okuneva, I.B., Characteristics of Vegetative Reproduction of Lilac Varieties, Extended Abstract of Cand. Sci. Dissertation, Tsytsin Main Moscow Botanical Garden, Moscow, 1998.

    Google Scholar 

  3. Andreev, L.N. and Gorbunov, Yu.N., Ex situ Conservation of Rare and Threatened Plants: Achievements and Problems, Izuchenie i okhrana raznoobraziya fauny, flory i osnovnykh ekosistem Evrazii (Study and Conservation of Fauna, Flora, and Main Ecosystems of Eurasia), Proc. Int. Conf., Moscow, 2000, pp. 19–23.

  4. Vysotskii, V.A., Biotechnological Methods in the Production System of Health-Improved Fruit-Berry Planting Stock, Extended Abstract of Doctoral Dissertation, All-Union Selection Technological Inst. of Horticulture and Hatchery, Moscow, 1998.

    Google Scholar 

  5. Molkanova, O.I., Stakheeva, T.S., Vasil’eva, O.G., et al., The Use of Biotechnological Methods for Reproduction and Conservation of Rare and Valued Plants, Botanicheskie sady kak tsentry sokhraneniya bioraznoobraziya i ratsional’nogo ispol’zovaniya rastitel’nykh resursov (Botanical Gardens as Centers of Conservation of Biodiversity and Vegetative Resources), Proc. Int. Sci. Conf. on the Occasion of the 60th Anniversary of the Tsytsin Main Moscow Botanical Garden of Academy of Sciences, Moscow, 2005, pp. 354–356.

  6. Butenko, R.G., Biologiya kletok vysshikh rastenii in vitro i biotekhnologiya na ikh osnove (Biology of Higher Plant Cells in vitro and the Based on It Biotechnology), Moscow: FBK-Press, 1999.

    Google Scholar 

  7. Dorokhov, D.B. and Kloke, E., Economic Technique for RAPD Analysis of Plant Genomes, Russ. J. Genet., 1997, vol. 33, no. 4, pp. 443–450.

    Google Scholar 

  8. Williams, J.G.K., Kubelik, A.P., Livak, K.J., et al., DNA Polymorphisms Amplified by Arbitrary Primers Are Useful as Genetic Markers, Nucleic Acids Res., 1990, vol. 18, no. 22, pp. 6531–6535.

    Article  PubMed  CAS  Google Scholar 

  9. Guerin, J.R., Sweeney, S.M., Collins, G., and Sedgley, M., The Development of a Genetic Database to Identify Olive Cultivars, Amer. Soc. Hort. Sci., 2002, vol. 127, no. 6, pp. 977–983.

    CAS  Google Scholar 

  10. Godwin, I.D., Sangduen, N., Kunanuvatchaidach, R., et al., RAPD Polymorphisms among Variant and Phenotypically Normal Rice (Oryza sativa var. indica) Somaclonal Progenies, Plant Cell Reports, 1997, vol. 16, no. 5, pp. 320–324.

    CAS  Google Scholar 

  11. Kudryavtsev, A.M., Martynov, S.P., Broggio, M., and Pukhalsky, V.A., Relevance of RAPD Analysis for Revealing Phylogenetic Relationships between Cultivars of Durum Wheat (T. durum Desf.), Russ. J. Genet., 2003, vol. 39, no. 9, pp. 1237–1246.

    Article  Google Scholar 

  12. Chen, X., Xiangyun, Z., and White, B.N., Analysis of Genetic Relationship among Lilacs (Syringa) by RAPD, Acta Horticulturae Sinica, 1995, vol. 22, no. 2, pp. 171–175.

    Google Scholar 

  13. Kochieva, E.Z., Ryzhova, N.N., Molkanova, O.I., et al., Genus Syringa: Molecular Markers of Species and Cultivars, Russ. J. Genet., 2004, vol. 40, no. 1, pp. 37–40.

    Article  Google Scholar 

  14. Edwards, S.K., Johonstone, C., and Thompson, C., A Simple and Rapid Method for the Preparation of Plant Genomic DNA for PCR Analysis, Nucleic Acids Res., 1991, vol. 19, p. 1349.

    Article  PubMed  CAS  Google Scholar 

  15. Rohlf, F.J., NTSYSpc: Numerical Taxonomy and Multivariate Analysis System, Version 2.02c, New York: Exeter Software, 1998.

    Google Scholar 

  16. Nei, M., Genetic Distance between Populations, Am. Natur., 1972, vol. 106, pp. 283–292.

    Article  Google Scholar 

  17. Dospekhov, B.A., Metodika polevogo opyta (Methods of Field Experiment), Moscow: Kolos, 1979.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    N. V. Melnikova, E. V. Borhert, V. P. Upelniek & A. M. Kudryavtsev

  2. Tsitsin Main Botanical Garden, Russian Academy of Sciences, Moscow, 127276, Russia

    I. B. Okuneva & O. I. Molkanova

  3. All-Russia Institute of Plant Industry, St. Petersburg, 190000, Russia

    S. P. Martynov

Authors
  1. N. V. Melnikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. V. Borhert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. P. Martynov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. B. Okuneva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. O. I. Molkanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. P. Upelniek
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. M. Kudryavtsev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. M. Kudryavtsev.

Additional information

Original Russian Text © N.V. Melnikova, E.V. Borhert, S.P. Martynov, I.B. Okuneva, O.I. Molkanova, V.P. Upelniek, A.M. Kudryavtsev, 2009, published in Genetika, 2009, Vol. 45, No. 1, pp. 97–103.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Melnikova, N.V., Borhert, E.V., Martynov, S.P. et al. Molecular genetic marker-based approaches to the verification of lilac Syringa vulgaris L. in vitro germplasm collections. Russ J Genet 45, 85–90 (2009). https://doi.org/10.1134/S1022795409010128

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795409010128

Keywords

Search

Navigation