Abstract
A collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work, 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively, and G ST value was 0.360. Genetic distance values calculated using Modified Rogers Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks.
Similar content being viewed by others
References
Hoisington, D., Khairallah, M., Reeves, T., et al., Plant Genetic Resources: What Can They Contribute towards Increased Crop Productivity?, Plants and Populations: Is There Time? NAS Colloquium, 1998.
Ajmone-Marsan, P., Castiglioni, P., Fusari, F., et al., Genetic Diversity and Its Relationship to Hybrid Performance in Maize as Revealed by RFLP and AFLP Markers, Theor. Appl. Genet, 1998, vol. 98, pp. 219–227.
Smith, O.S., Smith, J.S.C., Bowen, S.L., et al., Similarities among a Group of Elite Maize Inbreds as Measured by Pedigree, F1 Heterosis and RFLPs, Theor. Appl. Genet., vol. 80, pp. 833–840.
Dubreuil, P. and Charcosset, A., Genetic Diversity within and among Maize Populations: A Comparison between Isozyme and Nuclear RFLP Loci, Theor. Appl. Genet., 1998, vol. 96, pp. 577–587.
Rebourg, C., Dubreuil, P., and Charcosset, A., Genetic Diversity among Maize Populations: Bulk RFLP Analysis of 65 Accessions, Maydica, 1999, vol.44, pp.237–249.
Pejić, I., Ajmone-Marsan, P., Morgante, M., et al., Comparative Analysis of Genetic Similarity among Maize Inbred Lines Detected by RFLPs, RAPDs, SSRs, and AFLPs, Theor. Appl. Genet., 1998, vol. 97, pp. 1248–1255.
Garcia, A., Benchimol, L., Barbosa, A., et al., Comparison of RAPD, RFLP, AFLP and SSR Markers for Diversity Studies in Tropical Maize Inbred Lines, Genet. Mol. Biol., 2004, vol. 27, no. 4, pp. 579–588.
Verma, K.S., Khanna, V., and Singh, N., Random Amplified Polymorphic DNA Analysis of Indian Scented Basmati Rice (Oryza sativa L.) Germplasm for Identification of Variability and Duplicate Accessions, if any, Electrophoresis, 1999, vol. 20, pp. 1786–1789.
Virk, P.S., Newbury, H.J., Jackson, M.T., and Ford-Lloyd, B.V., The Identification of Duplicate Accessions within a Rice Germplasm Collection Using RAPD Analysis, Theor. Appl. Genet., 1995, vol. 90, pp. 1049–1055.
Carvalho, V.P., Ruas, C.F., Ferreira, J.M., et al., Genetic Diversity among Maize (Zea mays L.) Landraces Assessed by RAPD Markers, Genet. Mol. Biol., 2004, vol. 27, no. 2, pp. 228–236.
Demeke, T., Sasikumar, B., Huci, P., and Chibbar, R.N., Random Amplified Polymorphic DNA (RAPD) in Cereal Improvement, Maydica, 1997, vol. 42, pp. 133–142.
Karp, A., Edwards, K., Bruford, M., et al., Newer Molecular Technologies for Biodiversity Evaluation: Opportunities and Challenges, Nat. Biotechnol., 1997, vol. 15, pp. 625–628.
Reif, J.C., Xia, X.C., Melchinger, A.E., et al., Genetic Diversity Determined within and among CYMMIT Maize Populations of Tropical, Subtropical and Temperate Germplasm by SSR Markers, Crop Sci., 2004, vol. 44, pp. 326–334.
Beyene, Y., Botha, A., and Myburg, A., Genetic Diversity in Traditional Ethiopian Highland Maize Accessions Assessed by AFLP Markers and Morphological Traits, Biodiversity Conservation, 2006, vol. 15, pp. 2655–2671.
Smith, J.S.C., Chin, E.C.L., Shu, H., et al., An Evaluation of the Utility of SSR Loci as Molecular Markers in Maize (Zea mays L.): Comparisons with Data from RFLPs and Pedigree, Theor. Appl. Genet., 1997, vol. 95, pp. 163–173.
Reif, J.C., Hamrit, S., Heckenberger, M., et al., Genetic Structure and Diversity of European Flint Maize Populations Determined with SSR Analysis of Individuals and Bulks, Theor. Appl. Genet., 2005, vol. 111, pp. 906–913.
Radović, G., Muminović, J., and Jelovac, D., Local Maize Germplasm—Potentially Valuable Breeding Material: Maize and Sorghum Breeding at the End of 21st Century, EUCARPIA Genet., 2000, vol. 32, pp. 221–234.
Ignjatović-Micić, D., Ćorić, T., Kovčaević, D., et al., RFLP and RAPD Analysis of Maize (Zea mays L.) Local Populations for Identification of Variability and Duplicate Accessions, Maydica, 2003, vol. 48, pp. 153–159.
Dubreuil, P., Rebourg, C., Merlino, M., and Charcosset, A., The DNA-Pooled Sampling Strategy for Estimating the RFLP Diversity of Maize Populations, Plant Mol. Biol. Rep., 1999, vol. 17, pp. 123–138.
Saghai-Maroof, M., Soliman, K., Jorgensen, R., and Allard, R., Ribosomal DNA Spacer Length Polymorphism in Barley: Mendelian Inheritance, Chromosomal Location and Population Dynamics, Proc. Natl. Acad. Sci. USA, 1984, vol. 91, pp. 8014–8018.
Bassam, B.J., Caetano-Anolles, G., and Gresshoff, P.M., Fast and Sensitive Silver Staining of DNA in Polyacrylamide Gels, Anal. Biochem., 1991, vol. 196, pp. 80–83.
Nei, M., Estimation of Average Heterozygosity and Genetic Distance from a Small Number of Individuals, Genetics, 1978, vol. 89, pp. 583–590.
Nei, M., Genetic Distance between Populations, Am. Nat., 1973, vol. 106, pp. 282–292.
Rogers, J.S., Measures of Similarities and Genetic Distances, Studies in Genetics, Wheeler, M.R., Ed., Austin: Univ. of Texas Publ., 1972, VII, vol. 7213, pp. 145–153.
Wright, S., Evolution and Genetics of Populations, Chicago: Chicago Univ. Press, 1978.
Rebourg, C., Gouesnard, B., and Charcosset, A., Large-Scale Molecular Analysis of Traditional Maize Populations: Relationships with Morphological Variation, Heredity, 2001, vol. 86, pp. 574–584.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © D. Ignjatović-Micić, S. Mladenović Drinić, A. Nikolić, V. Lazić-Jančić, 2008, published in Genetika, 2008, Vol. 44, No. 11, pp. 1517–1524.
Rights and permissions
About this article
Cite this article
Ignjatović-Micić, D., Mladenović Drinić, S., Nikolić, A. et al. SSR analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories. Russ J Genet 44, 1317–1324 (2008). https://doi.org/10.1134/S1022795408110100
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795408110100