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Towards an integrated approach to study SNPs and expression of candidate genes associated with milk protein biosynthesis

  • Animal Genetics
  • Published:
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Abstract

MilkProtChip is oligonucleotide microarray allowing bovine genotyping based on single nucleotide polymorphisms (SNPs) in genes influencing milk protein biosynthesis. A total of 71 SNPs in 42 genes were selected as associated with milk protein biosynthesis. Genotyping of about 300 animals of Polish Black-and-White cattle showed that SNPs in acyl-CoA:1,2-diacylglycerol O-transferase (DGAT1), lactoferrin (LTF), casein kappa (CSN3) and growth hormone receptor (GHR) genes were associated with several milk performance traits. Analysis of correlations between SNPs and milk production traits showed that SNPs in single genes rarely affect the investigated traits. Only 4 of 42 investigated single SNPs had impact on milk production traits while 22 combinations of paired SNPs in these genes had impact. Positive effect SNP combinations in two genes can be a result of additive effect on these SNPs on the same traits or effect of genes interaction. The MilkBovExp chip representing 90 genes encoding transcription factors expressed in the bovine mammary gland and/or involved in mammary gland signaling pathways was designed for further investigation of impact of gene expression and/or its encoded products on milk traits performance.

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References

  1. Kamiński, S., Ruść, A., and Malewski, T., Single Nucleotide Polymorphism Database of Candidate Genes Associated with Cow Milk Protein, J. Anim. Feed Sci., 2004, vol. 13, pp. 51–64.

    Google Scholar 

  2. Kamiński, S., Ahman, A., Ruść, A., et al., MilkProt-Chip—a Microarray of SNPs in Candidate Genes Associated with Milk Protein Biosynthesis—Development and Validation, J. Appl. Genet., 2005, vol. 46, pp. 45–58.

    PubMed  Google Scholar 

  3. Jakob, E. and Puhan, Z., Technological Properties of Milk as Influenced by Genetic Polymorphism of Milk Protein (A Review), Int. Dairy J., 1992, vol. 2, pp. 157–178.

    Article  CAS  Google Scholar 

  4. Vonderhaar, B.K. and Ziska, S.E., Hormonal Regulation of Milk Protein Gene Expression, Annu. Rev. Physiol., 1989, vol. 51, pp. 641–652.

    Article  PubMed  CAS  Google Scholar 

  5. Coronini, R., de Looze, M.A., Puget, P., et al., Decoding the Literature on Genetic Variation, Nature Biotechnol., 2003, vol. 21, pp. 21–29.

    Article  CAS  Google Scholar 

  6. Ehrmann, S., Bartenschlager, H., and Geldermann, H., Quantitation of Gene Effects on Single Milk Protein in Selected Group of Dairy Cows, J. Anim. Breed Genet., 1997, vol. 114, pp. 121–132.

    CAS  Google Scholar 

  7. Kamiński, S. and Zabolewicz, T., Associations between Bovine Beta-Lactoglobulin Polymorphism within Coding and Regulatory Sequences and Milk Performance Traits, J. Appl. Genet., 2000, vol. 41, pp. 91–99.

    Google Scholar 

  8. Wagner, J.S., Schield, A.T., and Geldermann, H., DNA Variants within the 5′-Flanking Region of Milk-Protein Encoding Genes: II the Beta-Lactoglobulin-Encoding Gene, Theor. Appl. Genet., 1994, vol. 89, pp. 121–126.

    Article  CAS  Google Scholar 

  9. Kaminski, S., Association between Dde I Polymorphism within the Fragment of Bovine Kappa-Casein Promoter and Milk Performance Traits, J. Anim. Feed Sci., 2000, vol. 9, pp. 435–446.

    Google Scholar 

  10. Kamiński, S., Rymkiewicz-Schymczyk, J., Wojcik, E., and Ruść, A., Associations between Bovine Milk Protein Genotypes and Hapltypes and the Breeding Value of Polish Black-and-White Bulls, J. Anim. Feed Sci., 2002, vol. 11, pp. 205–221.

    Google Scholar 

  11. Kurg, A., Tonisson, N., Georgiou, I., et al., Arrayed Primer Extension: Solid-Phase Four-Color DNA Resequencing and Mutation Detection Technology, Genet. Testing, 2000, vol. 4, pp. 1–7.

    Article  CAS  Google Scholar 

  12. Schumaker, J.M., Metspalu, U., and Caskey, C.T., Mutation Detection by Solid Phase Primer Extension, Hum. Mutation, 1996, vol. 7, pp. 34–354.

    Google Scholar 

  13. Kamiński, S., Brym, P., Ruść, A., et al., Associations between Milk Performance Traits in Holstein Cows and 16 Candidate SNPs Identified by Arrayed Primer Extension (APEX) Microarray, Anim. Biotechnol., 2006, vol. 17, pp. 1–11.

    Article  PubMed  CAS  Google Scholar 

  14. Zheng, J., Ather, J.L., Sonstegard, T.S., and Kerr, D.E., Characterization of the Infection-Responsive Bovine Lactoferrin Promoter, Gene, 2005, vol. 353, no. 1, pp. 107–117.

    Article  PubMed  CAS  Google Scholar 

  15. Ip, S.C., Lau, J.S., Au, W.L., and Leung, F.C., Characterization of the 5′-Flanking Transcriptional Regulatory Region of Chicken Growth Hormone Gene, Exp. Biol. Med., 2004, vol. 229, no. 7, pp. 640–649.

    CAS  Google Scholar 

  16. Das, P., Meyer, L., Seyfert, H.M., et al., Structure of the Growth Hormone-Encoding Gene and Its Promoter in Mice, Gene, 1996, vol. 169, no. 2, pp. 209–213.

    Article  PubMed  CAS  Google Scholar 

  17. Hecht, C. and Geldermann, H., Variants within the 5′-Flanking Region and the Intron I of the Bovine Growth Hormone Gene, Anim. Genet., 1996, vol. 27, no. 5, pp. 329–332.

    PubMed  CAS  Google Scholar 

  18. Lum, L.S., Dovc, P., and Medrano, J.F., Polymorphisms of Bovine Beta-Lactoglobulin Promoter and Differences in the Binding Affinity of Activator Protein-2 Transcription Factor, J. Dairy Sci., 1997, vol. 80, no. 7, pp. 1389–1397.

    Article  PubMed  CAS  Google Scholar 

  19. Kuss, A.W., Gogol, J., and Geidermann, H., Associations of a Polymorphic AP-2 Binding Site in the 5′-Flanking Region of the Bovine Beta-Lactoglobulin Gene with Milk Proteins, J. Dairy Sci., 2003, vol. 86, no. 6, pp. 2213–2218.

    PubMed  CAS  Google Scholar 

  20. Ogg, S.L., Weldon, A.K., Dobbie, L., et al., Expression of Butyrophilin (Btn1a1) in Lactating Mammary Gland Is Essential for the Regulated Secretion of Milk-Lipid Droplets, Proc. Natl Acad. Sci. USA, 2004, vol. 101, no. 27, pp. 10084–10089.

    Article  PubMed  CAS  Google Scholar 

  21. Jack, L.J. and Mather, I.H., Cloning and Analysis of cDNA Encoding Bovine Butyrophilin, an Apical Glycoprotein Expressed in Mammary Tissue and Secreted in Association with the Milk-Fat Globule Membrane during Lactation, J. Biol. Chem., 1990, vol. 265, no. 24, pp. 14481–14486.

    PubMed  CAS  Google Scholar 

  22. Rowland, J.E., Lichanska, A.M., Kerr, L.M., et al., In vivo Analysis of Growth Hormone Receptor Signaling Domains and Their Associated Transcripts, Mol. Cell Biol., 2005, vol. 25, no. 1, pp. 66–77.

    Article  PubMed  CAS  Google Scholar 

  23. Master, S.R., Hartman, J.L., D’Cruz, C.M., et al., Functional Microarray Analysis of Mammary Organogenesis Reveals a Developmental Role in Adaptive Thermogenesis, Mol. Endocrinol., 2002, vol. 16, no. 6, pp. 1185–1203.

    Article  PubMed  CAS  Google Scholar 

  24. Visvader, J.E. and Lindeman, G.J., Transcriptional Regulators in Mammary Gland Development and Cancer, Int. J. Biochem. Cell Biol., 2003, vol. 35, no. 7, pp. 1034–1051.

    Article  PubMed  CAS  Google Scholar 

  25. Malewski, T., Kamiński, S., and Zwierzchowski, L., Gene Expression Profiling of Mouse Mammary Gland: Transition from Pregnancy to Lactation, Anim. Sci. Papers Rep., 2005, vol. 23, pp. 159–169.

    CAS  Google Scholar 

  26. Malewski, T., Gajewska, M., and Zwierzchowski, L., Changes in DNA-Binding Activity of Transcription Factors in the Differentiating Bovine Mammary Gland, Anim. Sci. Papers Rep., 2005, vol. 23, pp. 75–84.

    CAS  Google Scholar 

  27. Nielsen, H.B., Wernersson, R., and Knudsen, S., Design of Oligonucleotides for Microarrays and Perspectives for Design of Multi-Transcriptome Arrays, Nucleic Acids Res., 2003, vol. 31, pp. 3491–3496.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Animal Genetics, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-719, Poland

    S. Kamiński, A. Ruść & K. Oleński

  2. Department of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, Wolka Kosowska, 05-552, Poland

    T. Malewski

  3. AsperBiotech, Tartu, 51014, Estonia

    A. Ahman

  4. Department of Microbiology, Institute of Biology, Academia Podlaska, Siedlce, 08-110, Poland

    E. Wójcik & A. Jakubczak

  5. Laboratory of Molecular Genome Organization, Institute of Farm Animal Genetics and Breeding, Russian Academy of Agricultural Science, St. Petersburg-Pushkin, 196601, Russia

    A. A. Sazanov

  6. Department of Molecular and Biometrical Techniques, Museum and Institute of Zoology, Polish Academy of Sciences, Warszawa, 00-679, Poland

    T. Malewski

Authors
  1. S. Kamiński
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  2. T. Malewski
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  3. A. Ahman
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  4. E. Wójcik
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  5. A. Ruść
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  6. K. Oleński
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  7. A. Jakubczak
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  8. A. A. Sazanov
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Correspondence to S. Kamiński.

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This article was submitted by the authors in English.

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Kamiński, S., Malewski, T., Ahman, A. et al. Towards an integrated approach to study SNPs and expression of candidate genes associated with milk protein biosynthesis. Russ J Genet 44, 459–465 (2008). https://doi.org/10.1134/S1022795408040121

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  • DOI: https://doi.org/10.1134/S1022795408040121

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