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Effects of exogenous spermidine on antioxidant system of tomato seedlings exposed to high temperature stress

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Abstract

The effects of foliar spraying with spermidine (Spd) on antioxidant system in tomato (Lycopersicon esculentum Mill.) seedlings were investigated under high temperature stress. The high temperature stress significantly inhibited plant growth and reduced chlorophyll (Chl) content. Application of exogenous 1 mM Spd alleviated the inhibition of growth induced by the high temperature stress. Malondialdehyde (MDA), hydrogen peroxide (H2O2) content and superoxide anion (O2) generation rate were significantly increased by the high temperature stress, but Spd significantly reduced the accumulation of reactive oxygen species (ROS) and MDA content under the stress. The high temperature stress significantly decreased glutathione (GSH) content and activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR), but increased contents of dehydroascorbic acid (DHA), ascorbic acid (AsA), and oxidized glutathione (GSSG) in tomato leaves. However, Spd significantly increased the activities of antioxidant enzymes, levels of antioxidants and endogenous polyamines in tomato leaves under the high temperature stress. In addition, to varying degrees, Spd regulated expression of MnSOD, POD, APX2, APX6, GR, MDHAR, DHAR1, and DHAR2 genes in tomato leaves exposed to the high temperature stress. These results suggest that Spd could change endogenous polyamine levels and alleviate the damage by oxidative stress enhancing the non-enzymatic and enzymatic antioxidant system and the related gene expression.

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Abbreviations

APX:

ascorbate peroxidase

AsA:

ascorbic acid

CAT:

catalase

Chl:

chlorophyll

DHA:

dehydroascorbate

GR:

glutathione reductase

GSH:

reduced glutathione

GSSG:

oxidized glutathione

H2O2 :

hydrogen peroxide

MDA:

malondialdehyde

Pas:

polyamines

POD:

peroxidase

Put:

putrescine

SOD:

superoxide dismutase

Spd:

spermidine

Spm:

spermine

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Authors and Affiliations

  1. College of Horticulture, Nanjing Agricultural University, Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Jiangsu Province Engineering Laboratory for Modern Facility Agriculture Technology and Equipment, Nanjing, 210095, China

    Q. Q. Sang, S. Shu, X. Shan, S. R. Guo & J. Sun

  2. Academy of Protected Horticulture, Nanjing Agricultural University (Suqian), Jiangsu Suqian, 223800, China

    Q. Q. Sang, S. Shu, X. Shan, S. R. Guo & J. Sun

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  1. Q. Q. Sang
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  2. S. Shu
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  4. S. R. Guo
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Correspondence to S. R. Guo.

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Sang, Q.Q., Shu, S., Shan, X. et al. Effects of exogenous spermidine on antioxidant system of tomato seedlings exposed to high temperature stress. Russ J Plant Physiol 63, 645–655 (2016). https://doi.org/10.1134/S1021443716050113

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  • DOI: https://doi.org/10.1134/S1021443716050113

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