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Exogenous nitric oxide-mediated GSH-PC synthesis pathway in tomato under copper stress

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Abstract

Nitric oxide (NO) is a bioactive molecule that is extensively used at various biotic and abiotic stresses. This study investigated the law governing the variation of related enzymatic activity and metabolites in exogenous NO-mediated GSH-PC synthesis pathway in tomato solution culture subjected to copper stress. Results demonstrated that relative to control copper stress was more effective in the activation of γ-ECS and GS in tomato. Moreover, sharp increases in root GSH and PCs were observed, which keep upward as the process continued. Moreover, adding exogenous SNP (NO donor) can further improve γ-ECS and GS activities in tomato roots and facilitate the synthesis of GSH and PCs, thereby enhancing its peroxide removal ability, chelating excessive Cu2+, and reducing its biotoxicity. The GSH-PC metabolism in the tomato leaves lagged behind that in the roots to a certain extent. Although exogenous GSH synthesis inhibitor BSO inhibited γ-ECS activity in tomato roots, as well as GSH and PC syntheses, adding SNP can counteract this effect by lessening the influence to the PCs in leaves. Under copper stress, exogenous NO may stimulate a signaling mechanism and reduce the biotoxicity and oxidative damage caused by excessive Cu2+ through activating or enhancing the enzymatic and non-enzymatic systems in the GSH-PC synthesis pathway.

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Abbreviations

ASA:

Ascorbic acid

BSO:

L-buthionine-(S,R)-sulfoximine

DTNB:

5,5-dithio-bis(2-nitrobenzoic acid)

DTPA:

diethylenetriaminepentaacetic acid

GS:

glutathione synthetase

GSH:

reduced glutathione

GSSG:

oxidized glutathione

Hb:

bovine hemoglobin

H2O2:

hydrogen peroxide

NEM:

N-ethyl-maleimide

OPT:

o-phthalaldehyde

PCs:

phytochelatins

ROS:

reactive oxygen species

SNP:

sodium nitroprusside dihydrate

TAST:

total acid-soluble thiols

TG:

total glutathione [GSH + GSSG]

γ-ECs:

γ-glutamylcysteine synthetase

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Authors and Affiliations

  1. College of Resources and Environment, Shandong Agricultural University, Tai’an, 271018, China

    J. Wang, S. X. Yu, M. Zhang & X. M. Cui

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  1. J. Wang
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  2. S. X. Yu
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  3. M. Zhang
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  4. X. M. Cui
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Correspondence to J. Wang.

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Wang, J., Yu, S.X., Zhang, M. et al. Exogenous nitric oxide-mediated GSH-PC synthesis pathway in tomato under copper stress. Russ J Plant Physiol 62, 349–359 (2015). https://doi.org/10.1134/S1021443715030188

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  • DOI: https://doi.org/10.1134/S1021443715030188

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