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Effect of TiO2 nanoparticles on oxidative damage and antioxidant defense systems in chickpea seedlings during cold stress

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Abstract

The effects of TiO2 nanoparticles (NPs) on physiologo-biochemical responses were studied in two chickpea (Cicer arietimun L.) genotypes differing in cold sensitivity (tolerant Sel11439 and sensitive ILC533) during cold stress (CS). The results showed that hydrogen peroxide and MDA contents and electrolyte leakage index (ELI) increased under CS conditions in both genotypes and that these damage indices were higher in ILC533 than in Sel11439 plants. In plants treated with TiO2 NPs, a decreased H2O2 level was accompanied by a decrease in the MDA content and ELI compared to control plants, and these changes occurred more effectively in Sel11439 than in ILC533 plants. The antioxidant enzymes were more effective in cell protection against CS in Sel11439 plants compared to ILC533 plants, as well as in plants treated with TiO2 NPs compared to control plants. The lipoxygenase activity was induced efficiently only in Sel11439 plants treated with TiO2 NPs during CS, probably indicating its role in stress response (which was confirmed by measuring allen oxide synthase activity). TiO2 NPs caused stability of chlorophyll and carotenoid contents during CS. Results suggest that TiO2 NPs confer an increased tolerance of chickpea plants to CS, decreasing the level of injuries and increasing the capacity of defense systems.

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Abbreviations

AOS:

allen oxide synthase

APX:

ascorbate peroxidase

CAT:

catalase

Chl:

chlorophyll

CS:

cold stress

ELI:

electrolyte leakage index

GPX:

guaiacol peroxidase

LOX:

lipoxygenase

NPs:

nanoparticles

PPO:

polyphenol oxidase

SOD:

superoxide dismutase

TiO2 :

titanium dioxide

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran

    R. Mohammadi & R. Maali-Amiri

  2. School of Applied Sciences, Biotechnology and Environmental Biology, RMIT University, Building 223, Level 1, Plenty Road, Bundoora, Victoria, 3083, Australia

    N. L. Mantri

Authors
  1. R. Mohammadi
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  2. R. Maali-Amiri
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  3. N. L. Mantri
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Correspondence to R. Maali-Amiri.

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Mohammadi, R., Maali-Amiri, R. & Mantri, N.L. Effect of TiO2 nanoparticles on oxidative damage and antioxidant defense systems in chickpea seedlings during cold stress. Russ J Plant Physiol 61, 768–775 (2014). https://doi.org/10.1134/S1021443714050124

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  • DOI: https://doi.org/10.1134/S1021443714050124

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