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Exogenous nitric oxide promotes waterlogging tolerance as related to the activities of antioxidant enzymes in cucumber seedlings

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Abstract

We investigated the effects of exogenous sodium nitroprusside (SNP), a nitric oxide (NO) donor, on growth of cucumber (Cucumis sativus L., cv. Jinyou No.1) seedlings and antioxidant enzyme activities in cucumber leaves under waterlogging stress. The growth of cucumber seedlings was significantly inhibited when plants were exposed to waterlogging, whereas shoot spraying with SNP significantly alleviated the inhibition of growth from this type of stress: height, fresh and dry weights of the flooded plants increased obviously. Waterlogging also caused the activation of the antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX)), the reduction of the chlorophyll content, and the accumulation of MDA and protein in leaves. It was found that SNP treatment further potentiated the antioxidant enzyme activities and maintained the chlorophyll and protein content during the entire water-logging period; however, it reduced the MDA content. Thus, NO protects plants from oxidative damage and promotes growth by activation of antioxidant enzymes in leaves in an extent sufficient for the alleviation of membrane injury. However, exogenous NO had no significant effects on cucumber seedlings growth and antioxidant enzyme activities under nonstress conditions.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

O ·−2 :

superoxide radical

ONOO− :

peroxynitrite

POD:

peroxidase

SNP:

sodium nitroprusside

SOD:

superoxide dismutase

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Authors and Affiliations

  1. School of Agriculture and Food Science, Zhejiang Agriculture & Forestry University, Lin’an, Zhejiang, 311300, China

    H. F. Fan, C. X. Du, L. Ding & Y. L. Xu

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  1. H. F. Fan
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  2. C. X. Du
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  3. L. Ding
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  4. Y. L. Xu
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Correspondence to C. X. Du.

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Fan, H.F., Du, C.X., Ding, L. et al. Exogenous nitric oxide promotes waterlogging tolerance as related to the activities of antioxidant enzymes in cucumber seedlings. Russ J Plant Physiol 61, 366–373 (2014). https://doi.org/10.1134/S1021443714030042

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