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Experimental installation for measurements of chlorophyll fluorescence, CO2 exchange, and transpiration of a detached leaf

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Abstract

An original experimental installation is described to illustrate the unit-based principle in the open-mode design construction of a modern system for the combined fluorometric and CO2/H2O gas exchange measurements in the studies of the light and dark reactions of photosynthesis and the transpiration of a detached leaf.

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Abbreviations

IR:

infrared (spectral range)

PAM:

pulse-amplitude-modulated (fluorometry)

PAR:

photosynthetically active radiation

PC:

personal computer

PSII:

photosystem II

References

  1. Oja, V.M., High-speed gas analyzer for the investigation of leaf photosynthesis kinetics, Sov. Plant Physiol., 1983, vol. 30, pp. 1045–1052.

    CAS  Google Scholar 

  2. Pärnik, T.R., Keerberg, O.F., and Yurisma, E.Ya., High-speed exposure cell for photosynthesis investigation using 14CO2, Sov. Plant Physiol., 1987, vol. 34, pp. 837–845.

    Google Scholar 

  3. Laisk, A., Kiirats, O., Eichelmann, H., and Oja, V., Gas exchange studies of carboxylation kinetics in intact leaves, Photosynthesis In Silico: Understanding Complexity from Molecules to Ecosystems, vol. 29, Ser. Advances in Photosynthesis and Respiration, Laisk, A., Nedbal, L., and Govindjee, Eds., Berlin: Springer-Verlag, 2009, pp. 151–162.

    Google Scholar 

  4. Voznesenskii, V.L., Use of CO2 gas analyzers in field investigations of gas exchange and its components in plants, Infrakrasnye gazoanalizatory v izuchenii gazoobmena rastenii (Infrared Gas Analyzers for Plant Gas Exchange Investigation), Nichiporovich, A.A., Ed., Moscow: Nauka, 1990, pp. 6–19.

    Google Scholar 

  5. Shcherbatyuk, A.S., Multichannel setups with CO2 gas analyzers for laboratory or field investigations, Infrakrasnye gazoanalizatory v izuchenii gazoobmena rastenii (Infrared Gas Analyzers for Plant Gas Exchange Investigation), Nichiporovich, A.A., Ed., Moscow: Nauka, 1990, pp. 38–54.

    Google Scholar 

  6. Sivkov, M.D. and Nazarov, S.K., Single-channel gas analyzer for measurement of photosynthesis and transpiration of plants in field investigations, Infrakrasnye gazoanalizatory v izuchenii gazoobmena rastenii (Infrared Gas Analyzers for Plant Gas Exchange Investigation), Nichiporovich, A.A., Ed., Moscow: Nauka, 1990, pp. 55–64.

    Google Scholar 

  7. Rozhkovskii, A.D., The simple method for calibration of infrared gas analyzer, Infrakrasnye gazoanalizatory v izuchenii gazoobmena rastenii (Infrared Gas Analyzers for Plant Gas Exchange Investigation), Nichiporovich, A.A., Ed., Moscow: Nauka, 1990, pp. 107–115.

    Google Scholar 

  8. Laisk, A.H., Kinetika fotosinteza i fotodykhaniya C 3-rastenii (Kinetics of Photosynthesis and Photorespiration in C3 Plants), Moscow: Nauka, 1977.

    Google Scholar 

  9. Caemmerer, S., Farquhar, G., and Berry, J., Biochemical model of C3 photosynthesis, Photosynthesis In Silico. Understanding Complexity from Molecules to Ecosystems, vol. 29, Ser. Advances in Photosynthesis and Respiration, Laisk, A., Nedbal, L., and Govindjii, Eds., Berlin: Springer-Verlag, 2009, pp. 209–230.

    Google Scholar 

  10. Laisk, A., Eichelmann, H., and Oja, V., Leaf C3 photosynthesis in silico: integrated carbon/nitrogen metabolism, Photosynthesis In Silico. Understanding Complexity from Molecules to Ecosystems, vol. 29, Ser. Advances in Photosynthesis and Respiration, Laisk, A., Nedbal, L., and Govindjii, Eds., Berlin: Springer-Verlag, 2009, pp. 295–322.

    Google Scholar 

  11. Voronin, P.Yu. and Fedoseeva, G.P., Stomatal control of photosynthesis in detached leaves of woody and herbaceous plants, Russ. J. Plant Physiol., 2012, vol. 59, pp. 281–286.

    Article  CAS  Google Scholar 

  12. Schreiber, U., Chlorophyll Fluorescence and Photosynthetic Energy Conversion: Simple Introductory Experiments with the TEACHING-PAM Chlorophyll Fluorometer, Effeltrich: Heinz Walz GmbH, 1997.

    Google Scholar 

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Correspondence to P. Yu. Voronin.

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Original Russian Text © P.Yu. Voronin, 2014, published in Fiziologiya Rastenii, 2014, Vol. 61, No. 2, pp. 291–296.

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Voronin, P.Y. Experimental installation for measurements of chlorophyll fluorescence, CO2 exchange, and transpiration of a detached leaf. Russ J Plant Physiol 61, 269–273 (2014). https://doi.org/10.1134/S1021443714020174

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  • DOI: https://doi.org/10.1134/S1021443714020174

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