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Construction of cyanobacterial-bacterial consortium on the basis of axenic cyanobacterial cultures and heterotrophic bacteria cultures for bioremediation of oil-contaminated soils and water ponds

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Abstract

The ways of the creation of cyanobacterial-bacterial communities with a high remediation potential for cleaning of oil-contaminated soils and water reservoirs are considered. A special methodology was elaborated for the obtaining of bacteriologically pure (axenic) cultures of cyanobacteria: Phormidium sp. K-1, Oscillatoria sp. A-2, and Oscillatoria sp. C-3. It was shown that 14 strains of bacteria associated with these cyanobacteria manifest a hydrocarbon-oxidizing activity. They belong to the genera Pseudomonas, Alcaligenes, Arthrobacter, and Bacillus. The usage of oil-degrading bacteria, which we isolated from water reservoirs or took from collections, allowed us to construct artificial cyanobacterial-bacterial communities with a high hydrocarbon-oxidizing activity. In field experiments on the landfill site Khimpromservis Aktobe, the high remediatory effect of cyanobacterial-bacterial associations between Phormidium sp. K-1 sp. and bacteria Pseudomonas stutzeri A1, Pseudomonas sp. N2, and P. alcaligenes A5 was demonstrated. The results obtained are the basis for the elaboration of microbiological technologies of environment protection using cyanobacteria.

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Authors and Affiliations

  1. Al-Farabi Kazakh National University, pr. Al-Farabi 71, Almaty, 050040, Kazakhstan

    A. A. Zhubanova, A. K. Ernazarova, G. K. Kaiyrmanova, B. K. Zayadan, I. S. Savitskaya, G. Zh. Abdieva, A. S. Kistaubaeva & N. Sh. Akimbekov

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  1. A. A. Zhubanova
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  2. A. K. Ernazarova
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  3. G. K. Kaiyrmanova
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  4. B. K. Zayadan
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  5. I. S. Savitskaya
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  6. G. Zh. Abdieva
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  7. A. S. Kistaubaeva
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  8. N. Sh. Akimbekov
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Correspondence to A. A. Zhubanova.

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Original Russian Text © A.A. Zhubanova, A.K. Ernazarova, G.K. Kaiyrmanova, B.K. Zayadan, I.S. Savitskaya, G.Zh. Abdieva, A.S. Kistaubaeva, N.Sh. Akimbekov, 2013, published in Fiziologiya Rastenii, 2013, Vol. 60, No. 4, pp. 588–595.

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Zhubanova, A.A., Ernazarova, A.K., Kaiyrmanova, G.K. et al. Construction of cyanobacterial-bacterial consortium on the basis of axenic cyanobacterial cultures and heterotrophic bacteria cultures for bioremediation of oil-contaminated soils and water ponds. Russ J Plant Physiol 60, 555–562 (2013). https://doi.org/10.1134/S1021443713040183

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  • DOI: https://doi.org/10.1134/S1021443713040183

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