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2,4,6-trinitrotoluene as a trigger of oxidative stress in Fagopyrum tataricum callus cells

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Abstract

Effect of 2,4,6-trinitrotoluene (TNT) on callus cells of Tartar buckwheat (Fagopyrum tataricum (L.) Gaertn.) was accompanied by six-electron reduction of ortho- or para-nitro groups of the xenobiotic with the production of 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT). It was discovered that the xenobiotic TNT impairs integrity of cell membrane, which apparently results from its one-electron reduction coupled with production of nitro radical-anion and superoxide anion.

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Abbreviations

2-ADNT:

2-amino-4,6-dinitrotoluene

4-ADNT:

4-amino-2,6-dinitrotoluene

DANT:

diaminonitrotoluenes

DAPI:

4,6-diamidino-2-phenylindole dihydrochloride

HADNT:

hydroxylaminodinitrotoluenes

PI:

propidium iodide

Tiron:

sodium 4,5-dihydroxybenzene-1,3-disulfonate

TNT:

2,4,6-trinitrotoluene

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Authors and Affiliations

  1. Kazan (Volga Region) Federal University, ul. Kremlevskaya 18, Kazan, Tatarstan, 420008, Russia

    E. A. Naumenko, A. A. Rodionov, O. N. Il’inskaya & R. P. Naumova

  2. Institute of Biochemistry and Biophysics, Kazan Research Center, Russian Academy of Sciences, Kazan, Tatarstan, 420008, Russia

    G. V. Sibgatullina & A. R. Mukhitov

Authors
  1. E. A. Naumenko
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  2. G. V. Sibgatullina
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  3. A. R. Mukhitov
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  4. A. A. Rodionov
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  5. O. N. Il’inskaya
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  6. R. P. Naumova
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Correspondence to E. A. Naumenko.

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Original Russian Text © E.A. Naumenko, G.V. Sibgatullina, A.R. Mukhitov, A.A. Rodionov, O.N. Il’inskaya, R.P. Naumova, 2013, published in Fiziologiya Rastenii, 2013, Vol. 60, No. 3, pp. 416–423.

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Naumenko, E.A., Sibgatullina, G.V., Mukhitov, A.R. et al. 2,4,6-trinitrotoluene as a trigger of oxidative stress in Fagopyrum tataricum callus cells. Russ J Plant Physiol 60, 404–410 (2013). https://doi.org/10.1134/S1021443713030102

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  • DOI: https://doi.org/10.1134/S1021443713030102

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