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Adaptogenic action of the complex of phenylpropanoids on Dioscorea deltoidea cell culture under abiotic stress

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Abstract

Biological activity of the extract from golden root (Rhodiola rosea L.) roots, containing the complex of phenylpropanoids (CPP), was studied on the cell culture of yam (Dioscorea deltoidea Wall) under normal conditions and abiotic stress. The high radical-binding capacity of CPP relative to anion- and hydroxyl-radicals was observed. Having a high level of antiradical protection, CPP at a high concentration(100 μM) exerted prooxidant effect, causing a decrease in D. deltoidea cell viability and a decrease in the activities of antioxidant enzymes: superoxide dismutase, guaiacol-dependent peroxidase, and catalase, with the exception of ascorbate peroxidase. At treatment with 100 μM CPP, oxidase (prooxidant) activity of peroxidase increased by three times. The low CPP concentration (2 μM) did not induce substantial changes in the activities of tested enzymes and also a substantial increase in the oxidase activity of peroxidase. Under conditions of oxidative stress induced by paraquat and high temperature, CPP manifested adaptogenic action, increasing cell viability; however, under hyperosmotic stress, it was not efficient. CPP was most efficient at a low concentration after cell pre-incubation with it for 5 days. In this case, the amount of primary and secondary POL products increased. Shortening pre-cultivation with CPP reduced its defensive effect.

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Abbreviations

APO:

ascorbate peroxidase

ARA:

antiradical activity

CAT:

catalase

CD:

conjugated dienes

CPP:

complex of phenylpropanoids

PO:

guaiacol-dependent peroxidase

SOD:

superoxide dismutase

TBARS:

thiobarbituric acid-reacting substances

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    L. A. Volkova, V. V. Urmantseva, A. B. Burgutin & A. M. Nosov

Authors
  1. L. A. Volkova
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  2. V. V. Urmantseva
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  3. A. B. Burgutin
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  4. A. M. Nosov
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Correspondence to L. A. Volkova.

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Original Russian Text © L.A. Volkova, V.V. Urmantseva, A.B. Burgutin, A.M. Nosov, 2013, published in Fiziologiya Rastenii, 2013, Vol. 60, No. 2, pp. 230–239.

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Volkova, L.A., Urmantseva, V.V., Burgutin, A.B. et al. Adaptogenic action of the complex of phenylpropanoids on Dioscorea deltoidea cell culture under abiotic stress. Russ J Plant Physiol 60, 235–243 (2013). https://doi.org/10.1134/S102144371301010X

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  • DOI: https://doi.org/10.1134/S102144371301010X

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