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Patterns of ISSR and REMAP DNA markers after cryogenic preservation of spring wheat calli by dehydration method

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Abstract

Inter-Simple Sequence Repeat (ISSR) and retrotransposon-microsatellite amplified polymorphism (REMAP) markers were applied to study the influence of successive steps of dehydration cryopreservation on DNA in recovered calli and regenerated plants of spring wheat (Triticum aestivum L.). The precultivation step had no influence on the genetic stability of plant material. After the dehydration step, a new fragment appeared in the REMAP profiles for one DNA sample of calli of Nv16 line. A fragment of similar length was observed in one DNA sample for calli regenerated after complete procedure of cryopreservation in liquid nitrogen (−196°C). However, in samples of calli cultured in vitro for two and four weeks after any type of treatments, the amplicon spectra exhibited no difference from those of starting materials. The amplicon profiles of plants regenerated from calli after successive steps of cryopreservation were also identical to the profiles of the mother plants.

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Abbreviations

AFLP:

Amplified Fragment Length Polymorphism

CTAB:

hexadecyltrimethylammonium bromide

ISSR:

Inter-Simple Sequence Repeat

LTR:

Long Terminal Repeat

MS:

Murashige and Skoog (medium)

PCR:

Polymerase Chain Reaction

RAPD:

Random Amplification of Polymorphic DNA

REMAP:

Retrotransposon Microsatellite Amplified Polymorphism

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    A. I. Solov’eva, Yu. I. Dolgikh, O. N. Vysotskaya & A. S. Popov

Authors
  1. A. I. Solov’eva
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  2. Yu. I. Dolgikh
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  3. O. N. Vysotskaya
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  4. A. S. Popov
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Correspondence to A. I. Solov’eva.

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Original Russian Text © A.I. Solov’eva, Yu.I. Dolgikh, O.N. Vysotskaya, A.S. Popov, 2011, published in Fiziologiya Rastenii, 2011, Vol. 58, No. 3, pp. 359–366.

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Solov’eva, A.I., Dolgikh, Y.I., Vysotskaya, O.N. et al. Patterns of ISSR and REMAP DNA markers after cryogenic preservation of spring wheat calli by dehydration method. Russ J Plant Physiol 58, 423–430 (2011). https://doi.org/10.1134/S1021443711030162

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  • DOI: https://doi.org/10.1134/S1021443711030162

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