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SAD, a stearoyl-acyl carrier protein desaturase highly expressed in high-oil maize inbred lines

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Abstract

As special maize with more than 6% oil concentration in the grain, high-oil maize has received increased interest recently. To date, little is known about the expressions of genes involved in fatty acid metabolism of high-oil maize. Stearoyl-acyl carrier protein desaturase (SAD) is a key enzyme that converts stearic acid to oleic acid. In this study, two-dimensional electrophoresis, gas chromatography, and real-time PCR were used to determine the expressions of SAD at three seed development stages in high-oil and normal maize inbred lines. SAD was significantly more abundantly expressed in high-oil maize than in normal maize, not only at the protein and mRNA levels, but also at the product level. These results suggested that a high expression of SAD may play an important role in increasing oil concentration in high-oil maize.

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Abbreviations

ACP:

acyl carrier protein

DAP:

days after pollination

2-DE:

two-dimensial electrophoresis

SAD:

stearoylacyl carrier protein desaturase

QTL:

quantitative trait locus

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Authors and Affiliations

  1. Hi-Tech Research Centre, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Zh. J. Liu

  2. National Maize Improvement Center of China, China Agricultural University, Beijing, 100094, China

    X. H. Yang & Y. Fu

Authors
  1. Zh. J. Liu
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  2. X. H. Yang
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  3. Y. Fu
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Corresponding author

Correspondence to Zh. J. Liu.

Additional information

Published in Russian in Fiziologiya Rastenii, 2009, Vol. 56, No. 5, pp. 783–790.

This text was submitted by the authors in English.

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Liu, Z.J., Yang, X.H. & Fu, Y. SAD, a stearoyl-acyl carrier protein desaturase highly expressed in high-oil maize inbred lines. Russ J Plant Physiol 56, 709–715 (2009). https://doi.org/10.1134/S1021443709050185

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  • DOI: https://doi.org/10.1134/S1021443709050185

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