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Effects of various iron supply on oxidative stress development and ferritin formation in the common ice plants

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Abstract

Mesembryanthemum crystallinum L. plants were grown from seeds in perlite. At the age of 4 weeks (juvenile plants) or 6 weeks (adult plants), they were transferred on nutrient media with different Fe3+ content brought in as Fe2(SO4)3—EDTA complex (pH 6.0): control, iron deficit, and iron “excess”. Adult plants grown in media differing in iron content were subjected to salinity (300 mM NaCl) during the last 8 days of growth. Biochemical analyses were performed after plant fixation in liquid nitrogen; simultaneously, the samples for electron microscopy were taken. Different content of available Fe3+ in medium, especially under salinity conditions, changed sharply the content of chlorophyll and proline, the rate of lipid peroxidation, the level of H2O2, the activities of antioxidant enzymes in the leaves and roots, the number and sizes of plastoglobules, and ferritin formation in plastids. Joint action of salinity and iron deficit enhanced oxidative stress development, whereas iron excess hampered oxidative reaction development, reduced the rate of lipid peroxidation, and increased the chlorophyll content. At iron excess, plastoglobule lysis in plastids did not occur, their number and sizes increased, and ferritin deposits appeared, whereas the latter were absent at iron deficit.

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Abbreviations

PO:

peroxidase

POL:

peroxidation of lipids

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    N. I. Shevyakova, B. Ts. Eshinimaeva, N. V. Paramonova & Vl. V. Kuznetsov

Authors
  1. N. I. Shevyakova
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  2. B. Ts. Eshinimaeva
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  3. N. V. Paramonova
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  4. Vl. V. Kuznetsov
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Correspondence to N. I. Shevyakova.

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Original Russian Text © N.I. Shevyakova, B.Ts. Eshinimaeva, N.V. Paramonova, Vl.V. Kuznetsov, 2009, published in Fiziologiya Rastenii, 2009, Vol. 56, No. 4, pp. 518–529.

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Shevyakova, N.I., Eshinimaeva, B.T., Paramonova, N.V. et al. Effects of various iron supply on oxidative stress development and ferritin formation in the common ice plants. Russ J Plant Physiol 56, 470–479 (2009). https://doi.org/10.1134/S1021443709040050

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