Abstract
Mesembryanthemum crystallinum L. plants were grown from seeds in perlite. At the age of 4 weeks (juvenile plants) or 6 weeks (adult plants), they were transferred on nutrient media with different Fe3+ content brought in as Fe2(SO4)3—EDTA complex (pH 6.0): control, iron deficit, and iron “excess”. Adult plants grown in media differing in iron content were subjected to salinity (300 mM NaCl) during the last 8 days of growth. Biochemical analyses were performed after plant fixation in liquid nitrogen; simultaneously, the samples for electron microscopy were taken. Different content of available Fe3+ in medium, especially under salinity conditions, changed sharply the content of chlorophyll and proline, the rate of lipid peroxidation, the level of H2O2, the activities of antioxidant enzymes in the leaves and roots, the number and sizes of plastoglobules, and ferritin formation in plastids. Joint action of salinity and iron deficit enhanced oxidative stress development, whereas iron excess hampered oxidative reaction development, reduced the rate of lipid peroxidation, and increased the chlorophyll content. At iron excess, plastoglobule lysis in plastids did not occur, their number and sizes increased, and ferritin deposits appeared, whereas the latter were absent at iron deficit.
Similar content being viewed by others
Abbreviations
- PO:
-
peroxidase
- POL:
-
peroxidation of lipids
- SOD:
-
superoxide dismutase
References
Gross, J., Stein, R.J., Fett-Netto, A.G., and Fett, J.P., Iron Homeostasis Related Genes in Rice, Genet. Mol. Biol., 2003, vol. 26, pp. 1415–4757.
Dobrovol’skii, V.V., Biospheric Cycles of Heavy Metals and Regulatory Role of Soil, Pochvovedenie, 1997, no. 4, pp. 442–449.
Kosegarten, H., Hoffman, B., Rroco, E., Grolig, F., Glusenkamp, K.-H., and Mengel, K., Apoplastic pH and Fe(III) Reduction in Young Sunflower (Helianthus annuus) Roots, Physiol. Plant., 2004, vol. 122, pp. 95–106.
Ranieri, A., Castagna, A., Baldan, B., and Soldatini, G.F., Iron Deficiently Differently Affects Peroxidase Isoforms in Sunflower, J. Exp. Bot., 2001, vol. 52, pp. 25–35.
Marschner, H. and Römfeld, V., Strategies of Plants for Aquisition of Iron, Plant Soil, 1994, vol. 165, pp. 261–274.
Römfeld, V., Muller, C., and Marschner, H., Localization and Capacity of Proton Pumps in Roots of Intact Sunflower Plants, Plant Physiol., 1984, vol. 76, pp. 603–606.
Von Wiren, N., Marschner, H., and Römfeld, V., Uptake Kinetics of Iron-Phytosiderofores in Two Maize Genotypes Differing in Iron Efficiency, Plant Physiol., 1995, vol. 93, pp. 611–616.
Vert, G., Grotz, N., Dedaldechamp, F., Gaymard, F., Guerinot, M.L., Brat, J.F., and Curie, C., IRT1, an Arabidopsis Transporter Essential for Iron Uptake from the Soil and for Plant Growth, Plant Cell, 2002, vol. 14, pp. 1223–1233.
Connolly, E.L. and Querinot, M.L., Iron Stress in Plants, Gen. Biol., 2002, vol. 3, pp. 1465–1477.
Curie, C., Alonso, J.M., le Jean, M., Ecker, J.R., and Briat, J.F., Involvement of NRAMPI from Arabidopsis thaliana in Iron Transport, Biochem. J., 2000, vol. 347, pp. 749–755.
Pich, A., Manteuffel, R., Hilmer, S., Scholz, G., and Schmidt, W., Fe Homeostasis in Plant Cell: Does Nicotinamine Play Multiple Role in the Regulation of Cytoplasmic Fe Concentration? Planta, 2001, vol. 213, pp. 967–976.
Paramonova, N.V., Shevyakova, N.I., and Kuznetsov, Vl.V., Ultrastructure of Ferritin in the Leaves of Mesembryanthemum crystallinum under Stress Conditions, Russ. J. Plant Physiol., 2007, vol. 54, pp. 244–256.
Theil, E.C. and Hase, T., Plant and Microbial Ferritins, Iron Chelation in Plants and Soil Microorganisms, San Diego: Academic, 1993, pp. 133–156.
Theil, E.C. and Briat, J.-F., Plant Ferritin and Non-Heme Iron Nutrition in Humans, Harvest Plus Technical. Monograph 1, Washington, DC and Cali: International Food Policy Research Institute and International Center for Tropical Agriculture (CIAT), 2004, vol. 1, pp. 1–13.
Petit, J.-M., Briat, J.-F., and Lobreaux, S., Structure and Differential Expression of the Four Members of the Arabidopsis thaliana Ferritin Gene Family, Biochem. J., 2001, vol. 359, pp. 575–582.
Proudhon, D., Briat, J.-F., and Lescure, A.-M., Iron Induction of Ferritin Synthesis in Soybean Cell Suspension, Plant Physiol., 1989, vol. 90, pp. 586–590.
Szigeti, Z., Mechanism of Paraquat Resistance — from the Antioxidant Enzymes to the Transporters, Acta Biol. Szeged, 2005, vol. 49, pp. 177–179.
Bolann, B.F. and Ulvik, R.J., Release of Iron from Ferritin by Xanthine Oxidase. Role of the Superoxide Radical, Biochem. J., 1987, vol. 243, pp. 55–59.
Winter, K. and Holtum, J.A.M., The Effects of Salinity, Crassulacean Acid Metabolism and Plant Age on the Carbon Isotope Composition of Mesembryanthemum crystallinum L. a Halophytic C3-CAM Species, Planta, 2005, vol. 222, pp. 201–209.
Borrell, A., Carbonell, L., Farras, R., Puig-Perellada, P., and Tiburcio, A.F., Polyamines Inhibit Lipid Peroxidation in Senescing Oat Leaves, Physiol. Plant., 1997, vol. 99, pp. 385–390.
Bates, L.S., Waldren, R.P., and Teare, I.D., Rapid Determination of Free Proline for Water Stress Studies, Plant Soil, 1973, vol. 39, pp. 205–207.
Brennan, T. and Frenkel, C., Involvement of Hydrogen Peroxide in the Regulation of Senescence in Pear, Plant Physiol., 1997, vol. 59, pp. 411–416.
Radyukina, N.L., Shashukova, A.V., Shevyakova, N.I., and Kuznetsov, Vl.V., Proline Involvement in the Common Sage Antioxidant System in the Presence of NaCl and Paraquat, Russ. J. Plant Physiol., 2008, vol. 55, pp. 649–656.
Heath, R.L. and Parker, L., Photoperoxidation in Isolated Chloroplasts. I. Kinetics and Stoichiometry of Fatty Acid Peroxidation, Arch. Biochem. Biophys., 1968, vol. 125, pp. 189–198.
Esen, A., A Simple Method for Quantitative, Semiquantitative, and Qualitative Assay of Protein, Anal. Biochem., 1978, vol. 89, pp. 264–273.
Golubkina, N.A., Fluorometric Method for Selenium Determination, Zh. Anal. Khim., 1995, vol. 50, pp. 492–497.
Kuznetsov, Vl.V., Stetsenko, L.A., and Shevyakova, N.I., Exogenous Cadaverine Induces Oxidative Burst and Reduces Cadaverine Conjugate Content in the Common Ice Plant, J. Plant Physiol., 2008, www.sciencedirect.com.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © N.I. Shevyakova, B.Ts. Eshinimaeva, N.V. Paramonova, Vl.V. Kuznetsov, 2009, published in Fiziologiya Rastenii, 2009, Vol. 56, No. 4, pp. 518–529.
Rights and permissions
About this article
Cite this article
Shevyakova, N.I., Eshinimaeva, B.T., Paramonova, N.V. et al. Effects of various iron supply on oxidative stress development and ferritin formation in the common ice plants. Russ J Plant Physiol 56, 470–479 (2009). https://doi.org/10.1134/S1021443709040050
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1021443709040050