Abstract
To visualize phytohormone localization in plant tissues, transgenic plants comprising the GUS reporter gene are often used. However, until now only qualitative assessment of the hormone presence was available. In this work, we suggested the method for IAA quantification in transgenic DR5::GUS Arabidopsis thaliana L. plants by the analysis of digital images. An empirical quadratic dependence was established between the IAA concentration in medium and the level of GUS-dependent staining. Using this method, we demonstrated that, after A. thaliana root gravistimulation for 90 min, auxin lateral redistribution occurred. It resulted in the increase in the IAA concentration in the lower root part (in the elongation zone and apical meristem) by 200% on the average.
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Abbreviations
- GUS:
-
β-D-glucuronidase
- px:
-
pixel, a point in a digital image
- RGB:
-
color model where color is described as the combination of three basic components: red, green, and blue
- R, G, B:
-
brightness levels in red, green, and blue, respectively, in the digital image channels
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Original Russian Text © G.A. Pozhvanov, S.S. Medvedev, 2008, published in Fiziologiya Rastenii, 2008, Vol. 55, No. 5, pp. 786–792.
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Pozhvanov, G.A., Medvedev, S.S. Auxin quantification based on histochemical staining of GUS under the control of auxin-responsive promoter. Russ J Plant Physiol 55, 706–711 (2008). https://doi.org/10.1134/S1021443708050166
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DOI: https://doi.org/10.1134/S1021443708050166