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Vacuolar symplast and methodological approach to monitoring water self-diffusion between vacuoles of contacting root cells

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Abstract

New concepts of structural-functional organization of the transport system in higher plants were evolved at the current stage of investigations. In addition to the classical (cytoplasmic) symplast, another supra-cellular continuum was supposed to exist in the plant tissue, which interconnects vacuoles of neighboring cells through desmotubules and represents the second transport pathway within the plasmodesmata. This study describes and experimentally validates the method for monitoring the self-diffusion of water molecules between vacuoles of contacting cells in the maize (Zea mays L.) root by means of NMR method with a pulsed magnetic field gradient. The method is based on the fact that, at long period of self-diffusion observation, when water molecules in the apoplast and cytoplasm had already completed their relaxation and did not contribute significantly to the proton echo signal, the slope of the initial portion of the diffusional decay is independent of water permeability of the vacuolar membrane and is determined exclusively by water permeability of intervacuolar pathway through the desmotubules.

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Abbreviations

DD:

diffusional decay of spin echo

PMFG NMR:

pulsed magnetic field gradient NMR method

SDC(D, D eff):

self-diffusion coefficient

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Authors and Affiliations

  1. Kazan Institute of Biochemistry and Biophysics, Kazan Research Center, Russian Academy of Sciences, P.B. 30, Kazan, Tatarstan, 420111, Russia

    G. A. Velikanov

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  1. G. A. Velikanov
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Correspondence to G. A. Velikanov.

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Original Russian Text © G.A. Velikanov, 2007, published in Fiziologiya Rastenii, 2007, Vol. 54, No. 5, pp. 770–780.

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Velikanov, G.A. Vacuolar symplast and methodological approach to monitoring water self-diffusion between vacuoles of contacting root cells. Russ J Plant Physiol 54, 683–692 (2007). https://doi.org/10.1134/S1021443707050172

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  • DOI: https://doi.org/10.1134/S1021443707050172

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