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A role for SPINDLY gene in the regulation of oxidative stress response in Arabidopsis

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Abstract

SPINDLY (SPY) gene encodes a putative O-linked N-acetyl-glucosamine transferase, and yeast two-hybrid assay identified GIGANTEA (GI) as a SPY-interacting partner in Arabidopsis. GIGANTEA gene was previously shown to be involved in the regulation of oxidative stress response; however, it is unclear whether SPY gene is also involved in oxidative stress response. Here we showed that SPY plays a role in the regulation of the oxidative stress response. The spy-1 mutant was more tolerant to paraquat (PQ)-or hydrogen peroxide (H2O2)-mediated oxidative stress than wild-type plants. Analyses of endogenous H2O2 and superoxide anion radicals as well as lipid peroxidation revealed that enhanced tolerance of the spy-1 mutant to PQ-stress was not due to defects in the PQ uptake or the PQ sequestration from its site of action but rather the spy-1 mutation alleviated oxidative damage of plant cells upon PQ stress. Higher constitutive activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in spy-1 are more likely to be due to activation of both CSD2 gene encoding chloroplast Cu/Zn SOD and APX1 gene. Taken together, these results suggest that enhanced tolerance of the spy-1 mutant to oxidative stress is associated, at least in part, with constitutive activation of CSD2 and APX1.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

MDA:

malondialdehyde

MS:

Murashige and Skoog nutrient medium

PQ:

paraquat

ROS:

reactive oxygen species

RT-PCR:

reverse transcription PCR

SOD:

superoxide dismutase

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Authors and Affiliations

  1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui, 230009, China

    S. Q. Cao, M. Ye & Q. Huang

  2. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    R. X. Zhang

Authors
  1. S. Q. Cao
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  2. M. Ye
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Published in Russian in Fiziologiya Rastenii, 2006, Vol. 53, No. 4, pp. 604–611.

The text was submitted by the authors in English.

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Cao, S.Q., Ye, M., Huang, Q. et al. A role for SPINDLY gene in the regulation of oxidative stress response in Arabidopsis . Russ J Plant Physiol 53, 541–547 (2006). https://doi.org/10.1134/S1021443706040170

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