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Influence of the human blood serum on contractility and β-adrenoreactivity of the isolated human myocardium

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Abstract

Influence of adrenaline (10−9 to 10−4 g/ml) on the contraction amplitude caused by electrostimuli (1Hz, 5 ms, 25–30 V) and inotropic and adrenomodulation activities of blood serum of nonpregnant women (at dilutions of 1 : 10 000, 1 : 1000, 1 : 500, 1 : 100, 1 : 50, 1 : 10, and 1 : 5) have been studied. The study has been carried out on isolated myocardium strips of the right atrial auricle that were taken from 43 patients with ischemic illness of the heart and 9 patients with valvular heart diseases of various etiologies upon venous cannula insertion during an aortocoronary bypass. Direct dependence of the contraction amplitude on the cardiac output according to Teicholz has been found. This meant that strips of the right atrial auricle reflected the contractility of the left ventricle myocardium. Adrenaline has been shown to dose-dependently increase the amplitude of evoked contractions in the concentration interval from 10−7 to 10−6 g/ml and had no influence from 10−9 to 10−8 g/ml (dissociation constant, 2 × 10−7 g/ml), which proved a decrease in the β-adrenoreceptor’s (β-AR) activation. Blood serum in a dilution range from 1 : 10 000 to 1 : 50 had no effect on the contraction amplitude, but an enhanced effect has been found in a dilution range from 1 : 10 and 1 : 5. The presence of the endogenous activator of myocytes contractility (EAMC) has explained this enhanced effect. The β-adrenomodulation activity of blood serum has been explained by the presence of the endogenous sensitizer of β-AR (ESBAR) and the endogenous blocker of β-AR (EBBAR). The ESBAR activity of blood serum (dilutions: 1 : 1000, 1 : 500, 1 : 100, and 1 : 50) has been found in experiments with a subthreshold adrenaline concentration (10−8 g/ml). ESBAR (dilutions: 1 : 50 and 1 : 10) and EBBAR (dilution 1 : 500) activities of blood serum have been found in experiments with the maximum effective concentration of adrenaline (10−6 g/ml). Therefore, blood serum endogenous modulators of β-adrenergic reactivity, ESBAR and EBBAR, can modulate the activation of β-AR of human cardiomyocytes. These prove the prospects of the ESBAR analogue application in cardiology.

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Authors and Affiliations

  1. Vyatka State Humanities University, Kirov, 610002, Russia

    K. N. Korotayeva

  2. Kirov State Medical Academy, Kirov, 610027, Russia

    V. I. Tsirkin

  3. Kirov Regional Clinical Hospital, Kirov, 610027, Russia

    V. A. Vyaznikov

  4. Kirov Research Institute of Hematology and Blood Transfusion, Kirov, 610027, Russia

    A. A. Kostjaev

Authors
  1. K. N. Korotayeva
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  2. V. A. Vyaznikov
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  3. V. I. Tsirkin
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  4. A. A. Kostjaev
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Original Russian Text © K.N. Korotayeva, V.A. Vyaznikov, V.I. Tsirkin, A.A. Kostjaev, 2011, published in Fiziologiya Cheloveka, 2011, Vol. 37, No. 3, pp. 83–91.

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Korotayeva, K.N., Vyaznikov, V.A., Tsirkin, V.I. et al. Influence of the human blood serum on contractility and β-adrenoreactivity of the isolated human myocardium. Hum Physiol 37, 339–346 (2011). https://doi.org/10.1134/S0362119711020071

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