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Oncolytic Properties of a Mumps Virus Vaccine Strain in Human Melanoma Cell Lines

  • Molecular Cell Biology
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Abstract

The oncolytic potential of the attenuated mumps virus (MV) vaccine strain Leningrad-3 (L-3) was evaluated in a panel of four human metastatic melanoma cell lines. The lines were shown to be susceptible and permissive to MV infection. Efficient MV replication led to death of melanoma cells, but the effect differed among the cell lines. Possible mechanisms mediating the selectivity of MV L-3 towards the cell lines were explored. Replicative and oncolytic activity of MV was found to depend on the expression pattern of type I interferon genes. None of the melanoma cell lines showed induction of expression of the total spectrum of genes required to inhibit virus replication. Based on the results, MV L-3 was assumed to be a promising oncolytic agent for human melanoma cells.

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Abbreviations

qPCR:

quantitative PCR

TCD:

tissue cytopathic dose

ACTB :

β-actin gene

FC:

fold change

GAPDH :

glyceraldehyde 3-phosphate dehydrogenase gene

IFN-β:

interferon β

IFNAR1 :

interferon α/β receptor subunit 1

IFNB1 :

interferon β gene

ISG:

interferon-stimulated gene

ISG12 :

interferon-stimulated gene 12

ISG15 :

interferon-stimulated gene 15

MDA5:

melanoma differentiation-associated protein 5

MOI:

multiplicity of infection

MV:

mumps virus

MxA :

myxovirus resistance gene A

OAS1 :

2'-5'-oligoadenylate synthetase 1 gene

PGK1 :

phosphoglycerate kinase 1 gene

PKR:

protein kinase, RNA activated

RIG-I :

retinoic acid-inducible gene 1

STAT1 :

signal transducer and activator of transcription 1 gene

STAT2 :

signal transducer and activator of transcription 2 gene

TRAIL:

TNF-related apoptosis-inducing ligand

XAF-1:

X-linked inhibitor of apoptosis protein (XIAP)-associated factor 1

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Authors and Affiliations

  1. Mechnikov Institute of Vaccines and Sera, Moscow, 105064, Russia

    Y. I. Ammour, A. V. Milovanova, A. V. Sidorov & V. V. Zverev

  2. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, 115478, Russia

    O. O. Ryabaya

  3. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

    T. V. Nasedkina

  4. OOO Center of Pharmaceutical Analytics, Moscow, 117246, Russia

    I. E. Shohin

Authors
  1. Y. I. Ammour
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  2. O. O. Ryabaya
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  3. A. V. Milovanova
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  4. A. V. Sidorov
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  5. I. E. Shohin
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  6. V. V. Zverev
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  7. T. V. Nasedkina
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Corresponding author

Correspondence to Y. I. Ammour.

Additional information

Original Russian Text © Y.I. Ammour, O.O. Ryabaya, A.V. Milovanova, A.V. Sidorov, I.E. Shohin, V.V. Zverev, T.V. Nasedkina, 2018, published in Molekulyarnaya Biologiya, 2018, Vol. 52, No. 4, pp. 659–666.

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Ammour, Y.I., Ryabaya, O.O., Milovanova, A.V. et al. Oncolytic Properties of a Mumps Virus Vaccine Strain in Human Melanoma Cell Lines. Mol Biol 52, 570–576 (2018). https://doi.org/10.1134/S0026893318040027

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  • DOI: https://doi.org/10.1134/S0026893318040027

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