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Downregulation of human adenovirus DNA polymerase gene by modified siRNAs

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Abstract

Human adenoviruses, in particular D8, D19, and D37, cause ocular infections. Currently, there is no available causally directed treatment, which efficiently counteracts adenoviral infectious diseases. In our previous work, we showed that gene silencing by means of RNA interference is an effective approach for downregulation of human species D adenoviruses replication. In this study, we compared the biological activity of siRNAs and their modified analogs targeting human species D adenoviruses DNA polymerase. We found that one of selectively 2’-O-methyl modified siRNAs mediates stable and long-lasting suppression of the target gene (12 days post transfection). We suppose that this siRNA can be used as a potential therapeutic agent against human species D adenoviruses.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

    N. A. Nikitenko & V. S. Prassolov

  2. Heinrich Pette Institute–Leibniz Institute for Experimental Virology, Hamburg, D-20251, Germany

    T. Speiseder & T. Dobner

  3. Institute of Chemical Biology and Fundamental Medicine Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    E. L. Chernolovskaya & M. A. Zenkova

Authors
  1. N. A. Nikitenko
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  2. T. Speiseder
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  3. E. L. Chernolovskaya
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  4. M. A. Zenkova
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  5. T. Dobner
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  6. V. S. Prassolov
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Corresponding author

Correspondence to V. S. Prassolov.

Additional information

Original Russian Text © N.A. Nikitenko, T. Speiseder, E.L. Chernolovskaya, M.A. Zenkova, T. Dobner, V.S. Prassolov, 2016, published in Molekulyarnaya Biologiya, 2016, Vol. 50, No. 1, pp. 188–192.

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Nikitenko, N.A., Speiseder, T., Chernolovskaya, E.L. et al. Downregulation of human adenovirus DNA polymerase gene by modified siRNAs. Mol Biol 50, 164–167 (2016). https://doi.org/10.1134/S0026893316010155

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  • DOI: https://doi.org/10.1134/S0026893316010155

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