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Two Elovl5-like elongase genes in Cyprinus carpio var. Jian: Gene characterization, mRNA expression, and nutritional regulation

  • Genomics. Transcriptomics
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Abstract

Elovl5 elongase is a critical enzyme involved in the highly unsaturated fatty acid (HUFA) biosynthesis. There is very little information on the evolution and functional characterization of Elovl5-a and Elovl5-b genes in common carp (Cyprinus carpio var. Jian). In the present study, the genomic sequences and structures of two putative Elovl5-like elongase genes in the common carp genome were obtained. The mRNA expression patterns of Elovl5-a and Elovl5-b in tissues, hatching carp embryos, and juveniles under nutritional regulation were investigated. The results show that the two Elovl5 elongase genes have similar organization, coding 8 exons of high identity and introns of distinct size and sequence composition. They are not allelic variants of a single gene. Both Elovl5 elongase genes are highly expressed in liver, intestine (pyloric caeca) and brain. Elovl5-a and Elovl5-b mRNAs showed increased expression from newly hatched to 20 days after hatching. The regulation of Elovl5-a and Elovl5-b in response to dietary fatty acid composition was determined in liver, brain and intestine (pyloric caeca) of common carp fed with diets: (i) fish oil (FO) rich in n-3 HUFA, (ii) corn oil (CO, 18:2n-6) or (iii) linseed oil (LO, 18:3n-3). Also the differential expression of Elovl5-a and Elovl5-b genes in liver, brain and intestine in common carps fed with different oil sources was studied. Further work aimed at the determination of the mechanisms of differential expression of the Elovl5-a and Elovl5-b in different tissues and the roles of transcription factors in regulating HUFA synthesis is in progress.

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Abbreviations

CO:

corn oil

DAH:

days after hatching

DHA:

docosahexaenoic acid

EPA:

eicosapentaenoic acid

FO:

fish oil

hpf:

hours post fertilization

HUFA:

highly unsaturated fatty acids

LA:

linoleic acid

LNA:

α-linolenic acid

LO:

linseed oil

PUFA:

polyunsaturated fatty acids

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Authors and Affiliations

  1. Animal Science and Technology College, Henan University of Science and Technology, Luoyang, 471003, China

    H.-T. Ren & Y. Huang

  2. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China

    Y.-K. Tang, J.-H. Yu & P. Xu

Authors
  1. H.-T. Ren
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  2. Y. Huang
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  3. Y.-K. Tang
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  4. J.-H. Yu
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  5. P. Xu
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Correspondence to H.-T. Ren.

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The article is published in the original.

Published in Russian in Molekulyarnaya Biologiya, 2015, Vol. 49, No. 4, pp. 592–600.

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Ren, HT., Huang, Y., Tang, YK. et al. Two Elovl5-like elongase genes in Cyprinus carpio var. Jian: Gene characterization, mRNA expression, and nutritional regulation. Mol Biol 49, 527–534 (2015). https://doi.org/10.1134/S0026893315040135

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  • DOI: https://doi.org/10.1134/S0026893315040135

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