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Differential gene expression in incompatible interaction between Lilium regale Wilson and Fusarium oxysporum f. sp. lilii revealed by combined SSH and microarray analysis

  • Genomics. Transcriptomics
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Abstract

Fusarium wilt, caused by a soilborne pathogen Fusarium oxysporum f. sp. lilii, is the major disease of lily (Lilium L.). In order to isolate the genes differentially expressed in a resistant to F. oxysporum reaction in L. regale Wilson, a cDNA library was constructed with L. regale root during F. oxysporum infection using the suppression subtractive hybridization (SSH), and a total of 585 unique expressed sequence tags (ESTs) were obtained. Furthermore, the gene expression profiles in the incompatible interaction between L. regale and F. oxysporum were revealed by oligonucleotide microarray analysis of 585 unique ESTs in comparison to the compatible interaction between a susceptible Lilium Oriental Hybrid ‘Siberia’ and F. oxysporum. The result of expression profile analysis indicated that the genes encoding pathogenesis-related proteins (PRs), antioxidative stress enzymes, secondary metabolism enzymes, transcription factors, signal transduction proteins as well as a large number of unknown genes were involved in the early defense response of L. regale to F. oxysporum infection. Moreover, the following quantitative reverse transcription PCR (QRT-PCR) analysis confirmed reliability of the oligonucleotide microarray data. In the present study, isolation of differentially expressed genes in L. regale during response to F. oxysporum helped to uncover the molecular mechanism associated with the resistance of L. regale against F. oxysporum.

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References

  1. Liu D., He X., Li W., Chen C., Ge F. 2012. Molecular cloning of a thaumatin-like protein gene from Pyrus pyrifolia and overexpression of this gene in tobacco increased resistance to pathogenic fungi. Plant Cell Tissue Organ Cult. 111, 29–39.

    Article  CAS  Google Scholar 

  2. Lim J.H., Rhee H.K., Kim Y.J., Lim K.B., van Tuyl J.M. 2003. Resistance to Fusarium oxysporum f. sp. lilii in Lilium. In: Lee Jung-Myung., Zhang Donglin, Eds. Proc. XXVI Int. Horticult. Congress. Toronto, Canada: Can. Int. Dev. Agency, pp. 311–318.

    Google Scholar 

  3. Ortoneda M., Guarro J., Madrid M.P., Caracuel Z., Roncero M.I., Mayayo E., Di Pietro A. 2004. Fusarium oxysporum as a multihost model for the genetic dissection of fungal virulence in plants and mammals. Infect. Immun. 72, 1760–1766.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Berrocal-Lobo M., Molina A. 2008. Arabidopsis defense response against Fusarium oxysporum. Trends Plant Sci. 13, 145–150.

    Article  CAS  PubMed  Google Scholar 

  5. Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7, 405–410.

    Article  CAS  PubMed  Google Scholar 

  6. García-Limones C., Dorado G., Navas-Cortés J.A., Jiménez-Díaz R.M., Tena M. 2009. Changes in the redox status of chickpea roots in response to infection by Fusarium oxysporum f. sp. ciceris: Apoplastic antioxidant enzyme activities and expression of oxidative stress-related genes. Plant Biol. 11, 194–203.

    Article  PubMed  Google Scholar 

  7. Koornneef A., Pieterse C.M. 2008. Cross talk in defense signaling. Plant Physiol. 146, 839–844.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Silvar C., Merino F., Díaz J. 2009. Resistance in pepper plants induced by Fusarium oxysporum f. sp. lycopersici involves different defence-related genes. Plant Biol. 11, 68–74.

    Article  CAS  PubMed  Google Scholar 

  9. Lee O.R., Kim Y.J., Balusamy S.R., Khorolragchaa A., Sathiyaraj G., Kim M.K., Yang D.C. 2012. Expression of the ginseng PgPR10-1 in Arabidopsis confers resistance against fungal and bacterial infection. Gene. 506, 85–92.

    Article  CAS  PubMed  Google Scholar 

  10. Mahdavi F., Sariah M., Maziah M. 2012. Expression of rice thaumatin-like protein gene in transgenic banana plants enhances resistance to Fusarium wilt. Appl. Biochem. Biotechnol. 166, 1008–1019.

    Article  CAS  PubMed  Google Scholar 

  11. Liu D., Zhang X., Tu L., Zhu L., Guo X. 2006. Isolation by suppression-subtractive hybridization of genes preferentially expressed during early and late fiber development stages in cotton. Mol. Biol. 40, 825–834.

    CAS  Google Scholar 

  12. Lü G., Guo S., Zhang H., Geng L., Song F., Fei Z., Xu Y. 2011. Transcriptional profiling of watermelon during its incompatible interaction with Fusarium oxysporum f. sp. niveum. Eur. J. Plant Pathol. 131, 585–601.

    Article  Google Scholar 

  13. Bevan M., Bancroft I., Bent E., Love K., Goodman H., Dean C., Bergkamp R., Dirkse W., Van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E., Wambutt R., Weitzenegger T., Pohl T.M., Terryn N., Gielen J., Villarroel R., De Clerck R., Van Montagu M., Lecharny A., Auborg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.D., Rieger M., Schaeffer M., Funk B., Mueller-Auer S., Silvey M., James R., Montfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Düsterhöft A., Moores T., Jones J.D., Eneva T., Palme K., Benes V., Rechman S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.W., Klosterman S., Schueller C., Chalwatzis N. 1998. Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature. 391, 485–488.

    Article  CAS  PubMed  Google Scholar 

  14. Liu D., Liu Y., Rao J., Wang G., Li H., Ge F., Chen C. 2013. Overexpression of the glutathione S-transferase gene from Pyrus pyrifolia fruit improves tolerance to abiotic stress in transgenic tobacco plants. Mol. Biol. 47, 515–523.

    Article  CAS  Google Scholar 

  15. Schenk P.M., Kazan K., Wilson I., Anderson J.P., Richmond T., Somerville S.C., Manners J.M. 2000. Coordinated plant defense responses in Arabidopsis revealed by microarray analysis. Proc. Natl. Acad. Sci. U. S. A. 97, 11655–11660.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  16. Li Q., Chen F., Sun L.X., Zhang Z.Q., Yang Y.N., He Z.H. 2006. Expression profiling of rice genes in early defense responses to blast and bacterial blight pathogens using cDNA microarray. Physiol. Mol. Plant P. 68, 51–60.

    Article  CAS  Google Scholar 

  17. Dowd C., Wilson I.W., McFadden H. 2004. Gene expression profile changes in cotton root and hypocotyl tissues in response to infection with Fusarium oxysporum f. sp. vasinfectum. Mol. Plant Microbe Interact. 17, 654–667

    Article  CAS  PubMed  Google Scholar 

  18. Zhou X., Wu F. 2009. Differentially expressed transcripts from cucumber (Cucumis sativus L.) root upon inoculation with Fusarium oxysporum f. sp. cucumerinum Owen. Physiol. Mol. Plant P. 74, 142–150.

    Article  CAS  Google Scholar 

  19. Chadha P., Das R.H. 2006. A pathogenesis related protein, AhPR10 from peanut: An insight of its mode of antifungal activity. Planta. 225, 213–222.

    Article  CAS  PubMed  Google Scholar 

  20. Tobias D.J., Manoharan M., Pritsch C., Dahleen L.S. 2007. Co-bombardment, integration and expression of rice chitinase and thaumatin-like protein genes in barley (Hordeum vulgare cv. Conlon). Plant Cell Rep. 26, 631–639.

    Article  CAS  PubMed  Google Scholar 

  21. Wisser R.J., Kolkman J.M., Patzoldt M.E., Holland J.B., Yu J., Krakowsky M., Nelson R.J., Balint-Kurti P.J. 2011. Multivariate analysis of maize disease resistances suggests a pleiotropic genetic basis and implicates a GST gene. Proc. Natl. Acad. Sci. U. S. A. 108, 7339–7344.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  22. Naoumkina M.A., Zhao Q., Gallego-Giraldo L., Dai X., Zhao P.X., Dixon R.A. 2010. Genome-wide analysis of phenylpropanoid defence pathways. Mol. Plant Pathol. 11, 829–846.

    CAS  PubMed  Google Scholar 

  23. Xu L., Zhu L., Tu L., Liu L., Yuan D., Jin L., Long L., Zhang X. 2011. Lignin metabolism has a central role in the resistance of cotton to the wilt fungus Verticillium dahliae as revealed by RNA-Seq-dependent transcriptional analysis and histochemistry. J. Exp. Bot. 62, 5607–5621.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  24. Wang X., Liu W., Chen X., Tang C., Dong Y., Ma J., Huang X., Wei G., Han Q., Huang L., Kang Z. 2010. Differential gene expression in incompatible interaction between wheat and stripe rust fungus revealed by cDNA-AFLP and comparison to compatible interaction. BMC Plant Biol. 10, 9.

    Article  PubMed Central  PubMed  Google Scholar 

  25. Lebel E., Heifetz P., Thorne L., Uknes S., Ryals J., Ward E. 1998. Functional analysis of regulatory sequences controlling PR-1 gene expression in Arabidopsis. Plant J. 16, 223–233.

    Article  CAS  PubMed  Google Scholar 

  26. Chen W., Singh K.B. 1999. The auxin, hydrogen peroxide and salicylic acid induced expression of the Arabidopsis GST6 promoter is mediated in part by an ocs element. Plant J. 19, 667–677.

    Article  CAS  PubMed  Google Scholar 

  27. Zhang Y., Zhang G., Xia N., Wang X.J., Huang L., Kang Z.S. 2008. Cloning and characterization of a bZIP transcription factor gene in wheat and its expression in response to stripe rust pathogen infection and abiotic stresses. Physiol. Mol. Plant P. 73, 88–94.

    Article  CAS  Google Scholar 

  28. Heo W.D., Lee S.H., Kim M.C., Kim J.C., Chung W.S., Chun H.J., Lee K.J., Park C.Y., Park H.C., Choi J.Y., Cho M.J. 1999. Involvement of specific calmodulin isoforms in salicylic acid-independent activation of plant disease resistance responses. Proc. Natl. Acad. Sci. U. S. A. 96, 766–771.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  29. Cao A., Xing L., Wang X., Yang X., Wang W., Sun Y., Qian C., Ni J., Chen Y., Liu D., Wang X., Chen P. 2011. Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. Proc. Natl. Acad. Sci. U. S. A. 108, 7727–7732.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Authors and Affiliations

  1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China

    J. Rao, D. Liu, N. Zhang, H. He, F. Ge & C. Chen

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  1. J. Rao
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  2. D. Liu
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  3. N. Zhang
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  4. H. He
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  5. F. Ge
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  6. C. Chen
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Correspondence to D. Liu.

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Published in Russian in Molekulyarnaya Biologiya, 2014, Vol. 48, No. 6, pp. 915–926.

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Rao, J., Liu, D., Zhang, N. et al. Differential gene expression in incompatible interaction between Lilium regale Wilson and Fusarium oxysporum f. sp. lilii revealed by combined SSH and microarray analysis. Mol Biol 48, 802–812 (2014). https://doi.org/10.1134/S0026893314060144

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  • DOI: https://doi.org/10.1134/S0026893314060144

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