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Delayed augmentation effect of cytokine production after hyperthermia stimuli

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Abstract

Heatstroke is considered an important condition that may contribute to endothelial cell damage. The aim of this study was to assess temporal profiles of the cytokine (IL-6 and IL-8) and mRNA production when endothelial cells undergo higher temperature stimuli. In the first group, human umbilical vascular endothelial cells (HUVECs) were cultured at 4 different temperatures (37, 38, 39 or 40°C) for 1, 3 and 5 h. In the second group, HUVECs were cultured at 37°C for 4 h or 23 h, after stimulation by heating for one hour at the same culture temperatures used in the first group (37°C to 40°C). After culturing, IL-6 and IL-8 mRNA and protein levels were measured. It has been found the cytokine mRNA levels being significantly higher (p < 0.001) in all cells incubated at higher temperaturesthan those in the control (cultivation at 37°C). At the same time, the production of IL-6 and 8 at a higher temperature (39, 40°C) was significantly lower (p < 0.001) than at 37°C (control), and the decrease was temperature dependent. However, IL-6 and IL-8 levelswere significantly greater in the cells at 23 h after transient hyperthermic (40°C, 1 h) stimulation than in control ones (p < 0.001). After a transient hyperthermia, the production of the cytokinesin HUVECs is initially inhibited and then augmented. The results indicated that tissue injury might continue to develop after a hyperthermic event. There might be a potent risk for underestimation of cytokine induced tissue injury in the acute phase of a heatstroke.

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Authors and Affiliations

  1. Division of Emergency and Critical Care Medicine, Department of Acute Medicine, Nihon University School of Medicine, Tokyo, 173-8610, Japan

    K. Kinoshita, A. Sakurai, J. Yamaguchi, M. Furukawa & K. Tanjoh

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  1. K. Kinoshita
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  2. A. Sakurai
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  3. J. Yamaguchi
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  4. M. Furukawa
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  5. K. Tanjoh
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Correspondence to K. Kinoshita.

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Published in Russian in Molekulyarnaya Biologiya, 2014, Vol. 48, No. 3, pp. 429–435.

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Kinoshita, K., Sakurai, A., Yamaguchi, J. et al. Delayed augmentation effect of cytokine production after hyperthermia stimuli. Mol Biol 48, 371–376 (2014). https://doi.org/10.1134/S0026893314030108

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