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Inverse PCR-based site-directed mutagenesis of nucleotide sequences coding for carbohydrate-binding fragments of legume lectins

  • Applied Molecular Biology
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Abstract

A new method of site-directed mutagenesis was developed to allow manipulation with extended plasmid-cloned gene fragments irrespective of their position and the presence of restriction sites. The method was used to obtain chimeric constructs encoding a Pisum sativum lectin with the native carbohydrate-binding region replaced by its counterpart from other legumes. The method can be used in plasmid construction to clone a coding gene fragment under the control of a promoter in a certain reading frame.

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Authors and Affiliations

  1. Institute of Biochemistry and Genetics, Ufa Research Center, Russian Academy of Sciences, Ufa, Bashkortostan, 450054, Russia

    Al. Kh. Baymiev, I. I. Gubaydullin, An. Kh. Baymiev & A. V. Chemeris

Authors
  1. Al. Kh. Baymiev
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  2. I. I. Gubaydullin
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  3. An. Kh. Baymiev
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  4. A. V. Chemeris
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Corresponding author

Correspondence to Al. Kh. Baymiev.

Additional information

Original Russian Text © Al.Kh. Baymiev, I.I. Gubaydullin, An.Kh. Baymiev, A.V. Chemeris, 2007, published in Molekulyarnaya Biologiya, 2007, Vol. 41, No. 5, pp. 940–942.

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Baymiev, A.K., Gubaydullin, I.I., Baymiev, A.K. et al. Inverse PCR-based site-directed mutagenesis of nucleotide sequences coding for carbohydrate-binding fragments of legume lectins. Mol Biol 41, 857–859 (2007). https://doi.org/10.1134/S0026893307050202

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  • DOI: https://doi.org/10.1134/S0026893307050202

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